Antibody:	anti-NOS1 (R-20)
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	1A	Expression is in the superficial cortex.

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present		1B
TS21: hypothalamus	Present	Regionally restricted	1B	Expression is lateral.
TS21: telencephalon	Present	Regionally restricted	1B	Expression is in the amygdala.

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Present	Regionally restricted	1C	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Present	Regionally restricted	1D	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Present		1E

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Present		1F

Structure	Level	Pattern	Image	Note
TS23: superior colliculus	Present		2A

Structure	Level	Pattern	Image	Note
TS23: midbrain tegmentum	Present		2B

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	2C	Expression is in the reticular formation.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	2D	Expression is in the reticular formation.

Structure	Level	Pattern	Image	Note
TS25: pretectum	Present	Regionally restricted	2E	Expression is in the anterior pretectal nucleus.
TS25: brain	Present	Regionally restricted	2E	Expression is in the medial geniculate nucleus and the lateral habenular nuceus.
TS25: thalamus	Present		2E

Structure	Level	Pattern	Image	Note
TS25: pretectum	Present	Regionally restricted	2F	Expression is in the anterior pretectal nucleus.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	3F	Expression is in the raphe magnus nucleus.

Structure	Level	Pattern	Image	Note
TS26: caudate-putamen	Present		4C

Structure	Level	Pattern	Image	Note
TS26: brain	Present	Single cells	4D	Expression is in cells with dendrites-like beads.

Structure	Level	Pattern	Image	Note
TS27: brain	Present	Single cells	4E	Expression is in cells with dendrites-like beads.

Structure	Level	Pattern	Image	Note
TS27: brain	Present	Single cells	4F	Expression is in cells with dendrites-like beads.

Structure	Level	Pattern	Image	Note
TS21: neocortex	Moderate
TS21: pons	Moderate	Regionally restricted		Expression is in pontine nucleus.
TS21: medulla oblongata	Moderate
TS21: telencephalon	Strong	Regionally restricted		Expression is in the amygdala.
TS21: hypothalamus	Strong	Regionally restricted		Expression is strong in the lateral hypothalamic area, moderate in the ventral hypothalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Strong	Regionally restricted		Expression is in the peripeduncular nucleus.
TS23: superior colliculus	Strong
TS23: midbrain	Strong	Regionally restricted		Expression is in the reticular formation.
TS23: pons	Strong	Regionally restricted		Expression is in the posterior pontine neuroepithelium and is moderate in the anterior pons.
TS23: medulla oblongata	Strong	Regionally restricted		Strong in the medulla tegmentum, moderate in the medullary reticular formation and solitary nucleus, weak in the superior olivary nucleus.
TS23: telencephalon	Moderate	Regionally restricted		Expression is in the rhinencephalon.
TS23: amygdala	Moderate
TS23: neocortex	Moderate
TS23: cerebral cortex	Moderate	Regionally restricted		Expression is in the ventrobasal nucleus complex.
TS23: hypothalamus	Moderate	Regionally restricted		Expression is in the anterior hypothalamic neuroepithelium, lateral hypothalamic area, and the ventral hypothalamus.
TS23: tectum	Strong	Regionally restricted		Expression is in the inferior tectal neuroepithelium and is moderate in the superior tectal neuroepithelium.
TS23: medullary raphe	Weak
TS23: thalamus	Weak

Structure	Level	Pattern	Image	Note
TS25: superior colliculus	Strong
TS25: midbrain	Strong	Regionally restricted		Expression is in the anterior pretectal nucleus and in the reticular formation.
TS25: brainstem	Strong	Regionally restricted		Expression is in the superior central raphe nucleus and the median raphe nucleus.
TS25: pons	Strong	Regionally restricted		Expression is in the posterior pontine neuroepithelium.
TS25: amygdala	Moderate
TS25: cerebellum	Moderate
TS25: cerebral cortex subventricular zone	Moderate
TS25: corpus striatum	Moderate
TS25: inferior colliculus	Moderate
TS25: telencephalon septum	Moderate	Regionally restricted		Expression is in the lateral septum.
TS25: hypothalamus	Moderate	Regionally restricted		Expression is in the lateral hypothalamic area.
TS25: medulla oblongata	Moderate	Regionally restricted		Expression is in inferior olive, neuroepithelium, tegmentum and the reticular formation.
TS25: neocortex	Weak
TS25: thalamus	Weak	Regionally restricted		Expression is anterior and in the paraventricular thalamic nucleus, very strong in the medial geniculate nucleus.
TS25: epithalamus	Weak	Regionally restricted		Expression is in the lateral habenular nucleus.
TS25: diencephalon	Weak	Regionally restricted		Expression is in anterodorsal nucleus, rhomboid nucleus, and ventrolateral nucleuar complex.

Structure	Level	Pattern	Image	Note
TS26: periaqueductal grey	Strong
TS26: superior colliculus	Strong
TS26: neocortex	Moderate	Regionally restricted		Expression is in the occipital cortex and in parietal cortex.
TS26: amygdala	Moderate	Regionally restricted		Expression is in the anterior cortical-, anterior-, medial-, posterolateral cortex-, and basomedial amygdaloid nuclei, weak in the basolateral nucleus.
TS26: caudate-putamen	Moderate
TS26: inferior colliculus	Moderate
TS26: medullary raphe	Moderate
TS26: hypothalamus	Moderate	Regionally restricted		Expression is in the lateral hypothalamic area, weak in the anterior and the lateroanterior hypothalamic nucleus.
TS26: thalamus	Moderate	Regionally restricted		Strong in the medial geniculate nucleus, moderate in the lateral posterior-, weak in the centrolateral-, lateraldorsal-, subthalamic, paracentral-, and mediodorsal thalamic nuclei.
TS26: midbrain	Moderate	Regionally restricted		Expression is in the intercollicular nucleus.
TS26: brainstem nucleus	Moderate	Regionally restricted		Expression is in the dorsal raphe nucleus and is weak in the median raphe nucleus and ventral periolivary nucleus.
TS26: pons	Moderate	Regionally restricted		Expression is strong in the pedunculopontine tegmental nucleus, moderate in in the red nucleus, central gray area of pons, and the pontine reticular nucleus, weak in the parabrachial nucleus.
TS26: cortical subplate	Weak
TS26: main olfactory bulb	Weak
TS26: telencephalon septum	Weak
TS26: epithalamus	Weak	Regionally restricted		Expression is in the lateral habenular nucleus.
TS26: olfactory cortex	Weak	Regionally restricted		Expression is in the anterior olfactory nucleus.
TS26: basal ganglia	Weak	Regionally restricted		Expression is in globus pallidus.
TS26: diencephalon	Moderate	Regionally restricted		Expression is in the medial tubural nucleus.

Structure	Level	Pattern	Image	Note
TS27: dorsal raphe nucleus	Very strong
TS27: median raphe nucleus	Very strong
TS27: periaqueductal grey	Very strong
TS27: red nucleus	Very strong
TS27: brainstem nucleus	Very strong	Regionally restricted		Expression is weak in the paranigral nucleus and strong in the paralemniscal nucleus and the pedunculopontine tegmental nucleus.
TS27: raphe magnus nucleus	Weak
TS27: nucleus of trapezoid body	Moderate
TS27: gigantocellular reticular nucleus	Strong
TS27: inferior colliculus	Strong
TS27: medial geniculate nucleus	Strong
TS27: medulla oblongata	Strong	Regionally restricted		Expression is in the medullary raphe.
TS27: basomedial amygdaloid nucleus	Moderate
TS27: caudate-putamen	Moderate
TS27: cerebellum	Moderate
TS27: endopiriform nucleus	Moderate
TS27: lateral habenular nucleus	Moderate
TS27: lateral hypothalamic area	Moderate
TS27: paraventricular hypothalamic nucleus	Moderate
TS27: pontine nucleus	Moderate
TS27: pontine reticular formation	Moderate
TS27: superior colliculus	Moderate
TS27: thalamic nucleus	Moderate			Lateral posterior thalamic, paracentral, and peripeduncular nucleus.
TS27: deep cerebellar nucleus	Weak
TS27: frontal cortex	Weak
TS27: globus pallidus	Weak
TS27: medial amygdaloid nucleus	Weak
TS27: medial dorsal thalamic nucleus	Weak
TS27: medial habenular nucleus	Weak
TS27: parietal cortex	Weak
TS27: subcommissural organ	Weak
TS27: suprachiasmatic nucleus	Weak
TS27: ventral tegmental area	Weak