Probe:	Aporp1 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Present			Authors report expression first observed around 8.5 days p.c., peaking at 14-15 days p.c., and declining before birth.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present
TS17: future spinal cord	Present	Regionally restricted		Expression in spinal motor neurons from 10.5 days. p.c. onwards.

Structure	Level	Pattern	Image	Note
TS17: brain ventricular layer	Present		2B
TS17: hindbrain	Present		2B	Authors report expression is similar to that seen for mCOUP-TFI.
TS17: optic stalk	Present		2B	Authors report expression is similar to that seen for mCOUP-TFI.
TS17: telencephalon	Present		2B	Authors report expression is similar to that seen for mCOUP-TFI.
TS17: diencephalon	Present	Regionally restricted	2B	Slightly lower expression in the future D2 (dorsal thalamus) region of the diencephalon as compared to mCOUP-TFI.
TS17: midbrain	Present	Regionally restricted	2B	Expression restricted to rostral third of tectum.

Structure	Level	Pattern	Image	Note
TS19: optic stalk	Present		2D
TS19: telencephalon	Present	Regionally restricted	2D	Expression in dorsocaudal portion of telencephalon.
TS19: diencephalon	Present	Regionally restricted	2D	Higher expression in D1 (ventral thalamus and hypothalamus) and D2 (dorsal thalamus). Authors report expression is weaker in D2 than that of mCOUP-TFI.
TS19: midbrain	Present	Regionally restricted	2D	Medial domain was restricted to future midbrain. Rostrally restricted expression domains in the midbrain. Expression restricted to rostral third of tectum.
TS19: hindbrain	Present	Regionally restricted	2D	Caudal domain started in middle of rhombomere 1 and extended caudally.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	2F	Expression was more caudally restricted. No expression in the pallium.
TS22: ganglionic eminence	Strong		2F
TS22: lateral ganglionic eminence	Absent		2F
TS22: diencephalon	Present	Regionally restricted	2F	Expression extends from D1 (ventral thalamus and hypothalamus) anteriorly. Low levels in D3/D4 (pretectal) regions and zona limitans intrathalamica.
TS22: thalamus	Strong	Regionally restricted	2F	High expression in D1 (ventral thalamus and hypothalamus). Expression undetectable in D2 (dorsal thalamus).
TS22: hypothalamus	Strong		2F	High expression in D1 (ventral thalamus and hypothalamus). Expression undetectable in D2 (dorsal thalamus).
TS22: pretectum	Weak		2F	Expression slightly above background in the D3/D4 region.
TS22: midbrain	Present	Regionally restricted	2F	Expression in rostral part of tectum.
TS22: midbrain tegmentum	Strong	Regionally restricted	2F	High expression in oculomotor nucleus.

Structure	Level	Pattern	Image	Note
TS24: midbrain	Present	Regionally restricted		From 16.5 days p.c. onward, expression domains narrowed rostrally.
TS24: midbrain tegmentum	Strong	Regionally restricted		Strong expression in the oculomotor nuclues in the tegmentum from 11.5 to 16.5 days p.c..

Structure	Level	Pattern	Image	Note
TS26: brain	Weak	Regionally restricted	2H	Authors report expression is much lower than at previous stages.
TS26: telencephalon	Trace		2H	Minimal expression in the telencephalon.
TS26: diencephalon	Present	Regionally restricted	2H	Expression in D1 (ventral thalamus and hypothalamus), D3 and D4 (pretectal) regions, zona limitans intrathalamica and rostral midbrain.
TS26: midbrain	Present	Regionally restricted	2H	Expression in D1 (ventral thalamus and hypothalamus), D3 and D4 (pretectal) regions, zona limitans intrathalamica and rostral midbrain.
TS26: pretectum	Present		2H
TS26: thalamus	Present	Regionally restricted	2H	Expression extends from D1 anteriorly. Low expression in D2.
TS26: tectum	Present	Regionally restricted	2H	Expression restricted to an anterior strip of the tectum.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Strong	Regionally restricted	3B	High levels of expression in a portion of the hindbrain neurothelium just lateral to the floor plate. Background level of expression in the rest of the hindbrain neurothelium.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	3D	Expression in spinal cord motor neurons of the ventral horn from 10.5 days. p.c. onwards. Expression in substantia gelatinosa in the dorsal horn at the cervical level between 14.5 and 18.5 days p.c..

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present	Regionally restricted		Expression disappeared from somatic motor neurons at 16 days p.c. Authors state that expression persisted longer in the lateral horn.

Structure	Level	Pattern	Image	Note
TS26: dorsal grey horn	Present	Regionally restricted	3F	Expression in substantia gelatinosa in the dorsal horn at the cervical level between 14.5 and 18.5 days p.c..