Probe:	Erbal3 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Present			Expression first observed around 8.5 days p.c., peaking at 14-15 days p.c., and declining before birth.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present
TS17: future spinal cord	Present	Regionally restricted		Expression in spinal motor neurons from 10.5 days. p.c. onwards.

Structure	Level	Pattern	Image	Note
TS17: brain ventricular layer	Present		2A
TS17: telencephalon	Present	Regionally restricted	2A	Expression in dorsocaudal part of the telencephalon.
TS17: diencephalon	Present		2A
TS17: hindbrain	Present		2A
TS17: optic stalk	Present		2A
TS17: midbrain	Present	Regionally restricted	2A	Medial expression domain restricted to the midbrain. Anterior-posterior gradient of expression in tectum, highest at the rostral end.

Structure	Level	Pattern	Image	Note
TS19: optic stalk	Present		2C
TS19: telencephalon	Present	Regionally restricted	2C	Expression in dorsocaudal portion of telencephalon,
TS19: diencephalon	Present	Regionally restricted	2C	Anterior expression domain extends caudally into diencephalon, with segmentally restricted expression in diencephalic neuromeres. Similar high levels of expression in both D1 (ventral thalamus and hypothalamus) and D2 (dorsal thalamus) regions.
TS19: midbrain	Present	Regionally restricted	2C	Medial expression domain restricted to the midbrain. Anterior-posterior gradient of expression in tectum, highest at the rostral end.
TS19: hindbrain	Present	Regionally restricted	2C	Caudal domain started in rhombomere 1 and extended caudally.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present			Expression in most of the pallium.

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Strong		2E
TS22: medial ganglionic eminence	Strong		2E
TS22: diencephalon	Present	Regionally restricted	2E	Expression domain extends from D2 (dorsal thalamus) rostrally.
TS22: thalamus	Strong	Regionally restricted	2E	High levels of expression in D1 region (ventral thalamus and hypothalamus). No expression in the zona limitans intrathalamica.
TS22: pretectum	Weak		2E	Low levels in D3/D4 (pretectal) regions
TS22: midbrain tegmentum	Trace	Regionally restricted	2E	Minimal expression in oculomotor nucleus.
TS22: midbrain	Present	Regionally restricted	2E	Medial expression domain restricted to the midbrain.
TS22: tectum	Present	Regionally restricted	2E	Expression in tectum with an anterior posterior gradient of intensity, with the highest point at the rostral end.

Structure	Level	Pattern	Image	Note
TS24: midbrain	Present	Regionally restricted		From 16.5 days p.c. onward, expression domains narrowed rostrally.

Structure	Level	Pattern	Image	Note
TS26: forebrain	Weak	Regionally restricted	2G	Expression decreased approximately to that of background. Expression patterns were maintained until 18.5 days p.c.
TS26: telencephalon	Trace		2G	Minimal expression in the telencephalon.
TS26: pretectum	Present	Regionally restricted	2G	Expression in D1 (ventral thalamus and hypothalamus), D3 and D4 (pretectal) regions and zona limitans intrathalamica. High expression in D2 (dorsal thalamus)
TS26: thalamus	Present	Regionally restricted	2G	Expression in D1 (ventral thalamus and hypothalamus), D3 and D4 (pretectal) regions and zona limitans intrathalamica. High expression in D2 (dorsal thalamus)
TS26: tectum	Present	Regionally restricted	2G	Expression restricted to an anterior strip of the tectum.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Strong	Regionally restricted	3A	High levels of expression in a portion of the hindbrain neurothelium just lateral to the floor plate. Lower level of expression in the rest of the hindbrain neurothelium.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	3C	Expression in most regions of the spinal cord. Expression in spinal motor neurons from 10.5 days. p.c. onwards.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present	Regionally restricted		No expression in somatic motor neurons from 16 days p.c. Authors report expression persisted longer in the lateral horn.

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Present	Uniform	3E	Homogeneous expression in the entire spinal cord.