Probe:	Ets2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	1A	Expression is in the anterior two-thirds.

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present		1B
TS13: trunk unsegmented mesenchyme	Present		1B
TS13: neural tube	Present	Regionally restricted	1B	Expression is in the posterior neuropore.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	1C	In the rostral end of the presomitic mesoderm expression is in the anterior half of the two most rostral somitomeres, and in the anterior halves of the two recently segmented somites.
TS15: neural tube	Present	Regionally restricted	1C	Expression is in the posterior neural tube.

Structure	Level	Pattern	Image	Note
TS13: somite	Present	Regionally restricted	1D	There is regionalized expression in the anterior part of the newly segmented somite.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	1E	In the rostral end of the presomitic mesoderm expression is in the anterior half of the two most rostral somitomeres, and in the anterior halves of the two recently segmented somites.

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Regionally restricted	1F	Expression is in the rostral border of the segmenting somitomere and the newly formed somites.
TS19: neural tube	Present	Regionally restricted	1F	Expression is in the posterior neural tube.

Structure	Level	Pattern	Image	Note
TS20: somite	Present	Regionally restricted	1G	Expression is in the rostral border of the segmenting somitomere and the newly formed somites.
TS20: spinal cord	Present	Regionally restricted	1G	Expression is in the posterior neural tube.

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Present	Regionally restricted	1H	Expression is in the endothelium of intersegmental vessels.

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Present	Regionally restricted	1I	Expression is in the endothelium of intersegmental vessels.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Widespread	2A

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present		2B	Expression is in the posterior half of the limb bud

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	2C	Expression is as a crescent band of mesenchyme in the distal part of the limb bud and is weaker from the posterior to the anterior region. Also in the proximal anterior mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	2D	Expression is in the shoulder region.
TS20: forelimb mesenchyme	Present	Regionally restricted	2D	Expression is in the mesenchyme in the anterior and posterior margin.
TS20: hand	Present	Regionally restricted	2D	Expression is at the pre- and post-axial margin of digit I.

Structure	Level	Pattern	Image	Note
TS21: hindlimb phalanx pre-cartilage condensation	Present	Regionally restricted	2E	Expression is in the condensing mesenchyme that forms the perichondrium of the phalangeal cartilage.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	2F	Expression is as a crescent band of mesenchyme in the distal part of the limb bud and is weaker from the posterior to the anterior region.
TS16: forelimb bud ectoderm	Absent		2F

Structure	Level	Pattern	Image	Note
TS16: hindlimb bud mesenchyme	Present	Regionally restricted	2G	The expression domain extends ventromedially from the limb bud mesenchyme to the ventral body wall and stops short on either side of the tissues in the midline which extends from the cloaca to join the ventral ectodermal groove of the tail.
TS16: trunk mesenchyme	Present	Regionally restricted	2G	The expression domain extends ventromedially from the limb bud mesenchyme to the ventral body wall and stops short on either side of the tissues in the midline which extends from the cloaca to join the ventral ectodermal groove of the tail.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	2H	The expression domain extends ventromedially from the limb bud mesenchyme to the ventral body wall and stops short on either side of the tissues in the midline which extends from the cloaca to join the ventral ectodermal groove of the tail.
TS19: trunk mesenchyme	Present	Regionally restricted	2H	The expression domain extends ventromedially from the limb bud mesenchyme to the ventral body wall and stops short on either side of the tissues in the midline which extends from the cloaca to join the ventral ectodermal groove of the tail.

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	2I	Expression is in the hip.
TS20: hindlimb mesenchyme	Present	Regionally restricted	2I	The expression domain extends ventromedially from the limb bud mesenchyme to the ventral body wall and stops short on either side of the tissues in the midline which extends from the cloaca to join the ventral ectodermal groove of the tail.
TS20: trunk mesenchyme	Present	Regionally restricted	2I	The expression domain extends ventromedially from the limb bud mesenchyme to the ventral body wall and stops short on either side of the tissues in the midline which extends from the cloaca to join the ventral ectodermal groove of the tail.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	3A	Expression is in the dorsal epithelium.
TS17: forelimb bud	Present		3A
TS17: hindlimb bud	Present		3A

Structure	Level	Pattern	Image	Note
TS19: face	Present	Regionally restricted	3B	Expression is in the in the ectoderm in the distal portion of the face.
TS19: endolymphatic appendage epithelium	Present		3B
TS19: forelimb bud	Present		3B
TS19: hindlimb bud	Present		3B
TS19: dorsal root ganglion	Present	Regionally restricted	3B	Expression is in the dorsal (posterior) root ganglia.

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	3C	Expression is in the ventral surface ectoderm of the first and second arches.
TS20: endolymphatic appendage epithelium	Present		3C
TS20: forelimb	Present		3C
TS20: hindlimb	Present		3C
TS20: dorsal root ganglion	Present	Regionally restricted	3C	Expression is in the dorsal (posterior) root ganglia.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Present		3D
TS21: hindlimb	Present		3D
TS21: vibrissa	Present		3D

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		3E	Expression is in the dorsal (posterior) root ganglia.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Uniform	3F

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3G	Expression is in the dorsal neuroepithelium at the level of the hindlimb.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	3H	Expression is in the epithelium.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	3I	Expression is in the epithelium.

Structure	Level	Pattern	Image	Note
TS19: endolymphatic appendage	Present		3J