Tspan5 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:70837 Garcia-Frigola C, et al., Pattern of expression of the tetraspanin Tspan-5 during brain development in the mouse. Mech Dev. 2001 Aug;106(1-2):207-12
Assay type:	RNA in situ
MGI Accession ID:	MGI:5605328
Gene symbol:	Tspan5
Gene name:	tetraspanin 5

Probe:	Tspan5 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1A ©

Specimen 1A: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Strong		1A
TS24: neocortex	Strong		1A
TS24: thalamus	Present	Regionally restricted	1A	Expression is in some areas of the ventral thalamus.

1B ©

Specimen 1B: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong		1B
TS28: neocortex	Strong		1B

1C ©

Specimen 1C: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb mitral cell layer	Present		1C

1D ©

Specimen 1D: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: forebrain	Present	Regionally restricted	1D	Expression is in the dentate gyrus.
TS24: hippocampus	Present	Regionally restricted	1D	Expression is in the pyramidal cell layer.

1E ©

Specimen 1E: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Very strong		1E

1F ©

Specimen 1F: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		1F
TS28: main olfactory bulb mitral cell layer	Present		1F

1G ©

Specimen 1G: postnatal day 0 (more

)

Structure	Level	Image
TS27: dentate gyrus granule cell layer	Present	1G
TS27: dentate gyrus hilus	Present	1G
TS27: hippocampus pyramidal cell layer	Present	1G

1H ©

Specimen 1H: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: neocortex layer	Very strong	Regionally restricted	1H	Expression is in the subplate.

1I ©

Specimen 1I: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: nucleus of lateral olfactory tract	Present		1I

1J ©

Specimen 1J: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Strong	Regionally restricted	1J	Expression is in the diagonal band of Broca, in the septal area.

1K ©

Specimen 1K: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Moderate		1K

2A ©

Specimen 2A: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Present	Regionally restricted	2A	Expression is in the rhombic lip, in migrating external granular cells, in the deep cerebellar nuclei, and in migrating Purkinje cells.

2B ©

Specimen 2B: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present		2B
TS27: deep cerebellar nucleus	Present		2B

2C ©

Specimen 2C: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		2C

2D ©

Specimen 2D: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: superior colliculus	Present		2D
TS24: midbrain tegmentum	Present		2D	Including the periaqueductal ventricular zone.

2E ©

Specimen 2E: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: superior colliculus	Present		2E

2F ©

Specimen 2F: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: superior colliculus	Present		2F

2G ©

Specimen 2G: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Present	Regionally restricted	2G	Expression is in the rhombic lip, in migrating external granular cells, in the deep cerebellar nuclei, and in migrating Purkinje cells.

2H ©

Specimen 2H: postnatal day 10 (more

)

Structure	Level	Image
TS28: cerebellum Purkinje cell layer	Present	2H
TS28: cerebellum external granule cell layer	Present	2H
TS28: cerebellum internal granule cell layer	Present	2H

2I ©

Specimen 2I: postnatal day 21 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		2I

2J ©

Specimen 2J: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Present		2J
TS22: thalamus	Present	Regionally restricted	2J	Expression is in the ventral thalamus.

2K ©

Specimen 2K: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: pontine nucleus	Very strong		2K

3A ©

Specimen 3A: embryonic day 10.0 (more

)

Structure	Level	Image
TS16: future forebrain	Strong	3A
TS16: heart	Strong	3A
TS16: central nervous system	Present	3A
TS16: forelimb bud ectoderm	Present	3A
TS16: forelimb bud mesenchyme	Present	3A
TS16: hindlimb bud ectoderm	Present	3A
TS16: hindlimb bud mesenchyme	Present	3A
TS16: otocyst	Present	3A

3B ©

Specimen 3B: embryonic day 10.0 (more

)

Structure	Level	Pattern	Image	Note
TS16: heart	Present		3B

3C ©

Specimen 3C: embryonic day 10.0 (more

)

Structure	Level	Pattern	Image	Note
TS16: heart atrium	Present		3C
TS16: primitive ventricle	Present		3C

Specimen not shown: embryonic day 13.0 (more

)

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present
TS21: forelimb epithelium	Present
TS21: forelimb mesenchyme	Present
TS21: heart	Present
TS21: hindlimb epithelium	Present
TS21: hindlimb mesenchyme	Present
TS21: inner ear	Present
TS21: physiological umbilical hernia	Present

Specimen Table 1 E14: embryonic day 14.0 (more

)

Structure	Level	Pattern	Note
TS22: hippocampus	Very strong
TS22: neocortex	Very strong
TS22: forebrain	Very strong	Regionally restricted	Expression is in the dentate gyrus.
TS22: hypothalamus	Strong	Regionally restricted	Expression is strong in the estria terminalis nuclei, weak in the ventral nuclei.
TS22: epithalamus	Strong
TS22: superior colliculus	Strong
TS22: cerebellum	Strong	Regionally restricted	Expression is in the upper rhombic lip, ventricular zone, deep cerebellar nuclei, external granular layer and Purkinje cell layer.
TS22: main olfactory bulb	Moderate	Regionally restricted	Expression is in the mitral cell layer.
TS22: thalamus	Moderate	Regionally restricted	Moderate expression in the ventral nuclei, absent in the dorsal nuclei.
TS22: medulla oblongata	Moderate	Regionally restricted	Expression is in the lower rhombic lip, 4th ventral ventricular zone, pontine migratory stream, and reticular formation, weak in the vestibular and cochlear nuclei.
TS22: ganglionic eminence	Moderate
TS22: pretectum	Moderate
TS22: cerebral cortex subventricular zone	Weak
TS22: midbrain tegmentum	Weak
TS22: piriform cortex	Weak

Specimen Table 1 E16: embryonic day 16.0 (more

)

Structure	Level	Pattern	Note
TS24: hippocampus	Very strong
TS24: neocortex	Very strong
TS24: forebrain	Very strong		Expression is in the dentate gyrus.
TS24: telencephalon septum	Strong	Regionally restricted	Expression is in the medial septum and is weak in the dorso-lateral septum.
TS24: hypothalamus	Strong	Regionally restricted	Expression is strong in the estria terminalis nuclei, moderate in the ventral nuclei.
TS24: superior colliculus	Strong
TS24: midbrain	Strong	Regionally restricted	Expression is strong in the parabigeminal nuclei, weak in the lemniscus.
TS24: cerebellum	Strong	Regionally restricted	Expression is in the upper rhombic lip, ventricular zone, deep cerebellar nuclei, external granular layer and Purkinje cell layer.
TS24: main olfactory bulb	Moderate	Regionally restricted	Expression is in the mitral cell layer and weak in the granular cell layer.
TS24: medulla oblongata	Moderate	Regionally restricted	Expression is in the lower rhombic lip, 4th ventral ventricular zone, pontine migratory stream, inferior olive, and reticular formation, weak in the vestibular and cochlear nuclei.
TS24: amygdala	Moderate
TS24: epithalamus	Moderate
TS24: piriform cortex	Moderate
TS24: pretectum	Moderate
TS24: cerebral cortex	Moderate	Regionally restricted	Absent in the entorhinal cortex.
TS24: thalamus	Moderate	Regionally restricted	Moderate expression in the lateral geniculate nuclei and ventral nuclei, absent in the dorsal nuclei.
TS24: cerebral cortex subventricular zone	Weak
TS24: ganglionic eminence	Weak
TS24: midbrain tegmentum	Weak
TS24: inferior colliculus	Trace

Specimen Table 1 P0: postnatal day 0 (more

)

Structure	Level	Pattern	Note
TS27: cerebellum Purkinje cell layer	Very strong
TS27: dentate gyrus	Very strong
TS27: hippocampus CA1	Very strong
TS27: hippocampus CA2	Very strong
TS27: hippocampus CA3	Very strong
TS27: neocortex	Very strong
TS27: piriform cortex	Very strong
TS27: telencephalon septum	Very strong	Regionally restricted	Expression is in the medial septum/diagonal band. Weak in the dorso-lateral septum.
TS27: amygdala	Strong
TS27: cochlear nucleus	Strong
TS27: deep cerebellar nucleus	Strong
TS27: main olfactory bulb mitral cell layer	Strong
TS27: superior colliculus	Strong
TS27: cerebellum external granule cell layer	Moderate
TS27: cerebral cortex subventricular zone	Moderate
TS27: entorhinal cortex	Moderate
TS27: globus pallidus	Moderate
TS27: inferior colliculus	Moderate
TS27: lateral geniculate nucleus	Moderate
TS27: medial vestibular nucleus	Moderate
TS27: medullary reticular formation	Moderate
TS27: nucleus of lateral olfactory tract	Moderate
TS27: prethalamus	Moderate
TS27: hypothalamic nucleus	Moderate	Regionally restricted	Expression is in the ventral nuclei and in the estria terminalis nuclei.
TS27: dorsal thalamus	Moderate	Regionally restricted	Expression is in the dorsal thalamus, trace in the anterior ventral nuclei.
TS27: pons	Moderate	Regionally restricted	Expression is in the pontine migratory stream and is stronger in the pontine nuclei.
TS27: caudate-putamen	Weak
TS27: habenula	Weak
TS27: main olfactory bulb granule cell layer	Weak
TS27: pretectum	Weak
TS27: reticular thalamic nucleus	Weak
TS27: midbrain tegmentum	Weak	Regionally restricted	Expression is stronger in the parabigeminal nuclei.
TS27: brain	Weak	Regionally restricted	Expression is in the lemniscus.
TS27: brain ventricular layer	Weak	Regionally restricted	Expression is in the 4th ventricle ventricular zone.
TS27: medulla oblongata	Weak	Regionally restricted	Expression is in the inferior olive.
TS27: main olfactory bulb glomerular layer	Absent

Specimen Table 1 P5: postnatal day 5 (more

)

Structure	Level	Pattern	Note
TS28: cerebellum Purkinje cell layer	Very strong
TS28: dentate gyrus	Very strong
TS28: hippocampus CA1	Very strong
TS28: hippocampus CA2	Very strong
TS28: hippocampus CA3	Very strong
TS28: neocortex	Very strong
TS28: piriform cortex	Very strong
TS28: telencephalon septum	Very strong	Regionally restricted	Expression is in the medial septum/diagonal band. Weak in the dorso-lateral septum.
TS28: amygdala	Strong
TS28: cochlear nucleus	Strong
TS28: entorhinal cortex	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: parabigeminal nucleus	Strong
TS28: pontine nucleus	Strong
TS28: superior colliculus	Strong
TS28: hypothalamic nucleus	Moderate	Regionally restricted	Expression is in the ventral nuclei, weak in the estria terminalis nuclei.
TS28: cerebellum external granule cell layer	Moderate
TS28: cerebral cortex subventricular zone	Moderate
TS28: deep cerebellar nucleus	Moderate
TS28: dorsal thalamus	Moderate
TS28: globus pallidus	Moderate
TS28: lateral geniculate nucleus	Moderate
TS28: medial vestibular nucleus	Moderate
TS28: medullary reticular formation	Moderate
TS28: nucleus of lateral olfactory tract	Moderate
TS28: spinal vestibular nucleus	Moderate
TS28: anterior ventral thalamic nucleus	Weak
TS28: caudate-putamen	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: inferior colliculus	Weak
TS28: inferior olivary nucleus	Weak
TS28: main olfactory bulb glomerular layer	Weak
TS28: main olfactory bulb granule cell layer	Weak
TS28: prethalamus	Weak
TS28: reticular thalamic nucleus	Weak
TS28: subthalamic nucleus	Weak
TS28: brain	Weak	Regionally restricted	Expression is in the lemniscus.
TS28: habenula	Absent
TS28: midbrain tegmentum	Absent
TS28: pretectum	Absent

Specimen Table 1 P10: postnatal day 10 (more

)

Structure	Level	Pattern	Note
TS28: cerebellum Purkinje cell layer	Very strong
TS28: dentate gyrus	Very strong
TS28: entorhinal cortex	Very strong
TS28: hippocampus CA1	Very strong
TS28: hippocampus CA2	Very strong
TS28: hippocampus CA3	Very strong
TS28: neocortex	Very strong
TS28: piriform cortex	Very strong
TS28: telencephalon septum	Very strong	Regionally restricted	Expression is in the medial septum/diagonal band. Weak in the dorso-lateral septum.
TS28: amygdala	Strong
TS28: cochlear nucleus	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: parabigeminal nucleus	Strong
TS28: pontine nucleus	Strong
TS28: superior colliculus	Strong
TS28: cerebellum external granule cell layer	Moderate
TS28: cerebral cortex subventricular zone	Moderate
TS28: globus pallidus	Moderate
TS28: medial vestibular nucleus	Moderate
TS28: medullary reticular formation	Moderate
TS28: nucleus of lateral olfactory tract	Moderate
TS28: spinal vestibular nucleus	Moderate
TS28: anterior ventral thalamic nucleus	Weak
TS28: caudate-putamen	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: deep cerebellar nucleus	Weak
TS28: inferior colliculus	Weak
TS28: lateral geniculate nucleus	Weak
TS28: main olfactory bulb glomerular layer	Weak
TS28: main olfactory bulb granule cell layer	Weak
TS28: reticular thalamic nucleus	Weak
TS28: subthalamic nucleus	Weak
TS28: hypothalamic nucleus	Weak	Regionally restricted	Expression is in the ventral nuclei and in the estria terminalis nuclei.
TS28: brain	Weak	Regionally restricted	Expression is in the lemniscus.
TS28: inferior olivary nucleus	Trace
TS28: habenula	Absent
TS28: midbrain tegmentum	Absent
TS28: pretectum	Absent

Specimen Table 1 P15: postnatal day 15 (more

)

Structure	Level	Pattern	Note
TS28: cerebellum Purkinje cell layer	Very strong
TS28: dentate gyrus	Very strong
TS28: entorhinal cortex	Very strong
TS28: hippocampus CA1	Very strong
TS28: hippocampus CA2	Very strong
TS28: hippocampus CA3	Very strong
TS28: neocortex	Very strong
TS28: piriform cortex	Very strong
TS28: telencephalon septum	Very strong	Regionally restricted	Expression is in the medial septum/diagonal band. Weak in the dorso-lateral septum.
TS28: amygdala	Strong
TS28: cochlear nucleus	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: parabigeminal nucleus	Strong
TS28: pontine nucleus	Strong
TS28: superior colliculus	Strong
TS28: cerebral cortex subventricular zone	Moderate
TS28: globus pallidus	Moderate
TS28: medullary reticular formation	Moderate
TS28: nucleus of lateral olfactory tract	Moderate
TS28: trigeminal V spinal sensory nucleus	Moderate
TS28: caudate-putamen	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: deep cerebellar nucleus	Weak
TS28: dorsal thalamus	Weak
TS28: lateral geniculate nucleus	Weak
TS28: main olfactory bulb glomerular layer	Weak
TS28: main olfactory bulb granule cell layer	Weak
TS28: reticular thalamic nucleus	Weak
TS28: subthalamic nucleus	Weak
TS28: hypothalamic nucleus	Weak	Regionally restricted	Expression is in the ventral nuclei and in the estria terminalis nuclei.
TS28: brain	Weak	Regionally restricted	Expression is in the lemniscus.
TS28: anterior ventral thalamic nucleus	Trace
TS28: inferior olivary nucleus	Trace
TS28: medial vestibular nucleus	Trace
TS28: prethalamus	Trace
TS28: spinal vestibular nucleus	Trace
TS28: habenula	Absent
TS28: inferior colliculus	Absent
TS28: midbrain tegmentum	Absent
TS28: pretectum	Absent

Specimen Table 1 P21: postnatal day 21 (more

)

Structure	Level	Pattern	Note
TS28: cerebellum Purkinje cell layer	Very strong
TS28: dentate gyrus	Very strong
TS28: entorhinal cortex	Very strong
TS28: hippocampus CA1	Very strong
TS28: hippocampus CA2	Very strong
TS28: hippocampus CA3	Very strong
TS28: neocortex	Very strong
TS28: piriform cortex	Very strong
TS28: telencephalon septum	Very strong	Regionally restricted	Expression is in the medial septum/diagonal band. Weak in the dorso-lateral septum.
TS28: amygdala	Strong
TS28: cochlear nucleus	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: parabigeminal nucleus	Strong
TS28: pontine nucleus	Strong
TS28: superior colliculus	Strong
TS28: cerebral cortex subventricular zone	Moderate
TS28: globus pallidus	Moderate
TS28: medullary reticular formation	Moderate
TS28: nucleus of lateral olfactory tract	Moderate
TS28: trigeminal V spinal sensory nucleus	Moderate
TS28: caudate-putamen	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: deep cerebellar nucleus	Weak
TS28: dorsal thalamus	Weak
TS28: lateral geniculate nucleus	Weak
TS28: main olfactory bulb glomerular layer	Weak
TS28: main olfactory bulb granule cell layer	Weak
TS28: reticular thalamic nucleus	Weak
TS28: subthalamic nucleus	Weak
TS28: hypothalamic nucleus	Weak	Regionally restricted	Expression is in the ventral nuclei and in the estria terminalis nuclei.
TS28: anterior ventral thalamic nucleus	Trace
TS28: inferior olivary nucleus	Trace
TS28: medial vestibular nucleus	Trace
TS28: prethalamus	Trace
TS28: spinal vestibular nucleus	Trace
TS28: brain	Trace	Regionally restricted	Expression is in the lemniscus.
TS28: habenula	Absent
TS28: inferior colliculus	Absent
TS28: midbrain tegmentum	Absent
TS28: pretectum	Absent

Specimen Table 1 Adult: postnatal adult (more

)

Structure	Level	Pattern	Note
TS28: cerebellum Purkinje cell layer	Very strong
TS28: dentate gyrus	Very strong
TS28: entorhinal cortex	Very strong
TS28: hippocampus CA1	Very strong
TS28: hippocampus CA2	Very strong
TS28: hippocampus CA3	Very strong
TS28: neocortex	Very strong
TS28: piriform cortex	Very strong
TS28: telencephalon septum	Very strong	Regionally restricted	Expression is in the medial septum/diagonal band. Weak in the dorso-lateral septum.
TS28: amygdala	Strong
TS28: cochlear nucleus	Strong
TS28: main olfactory bulb mitral cell layer	Strong
TS28: parabigeminal nucleus	Strong
TS28: pontine nucleus	Strong
TS28: superior colliculus	Strong
TS28: cerebral cortex subventricular zone	Moderate
TS28: globus pallidus	Moderate
TS28: medullary reticular formation	Moderate
TS28: nucleus of lateral olfactory tract	Moderate
TS28: trigeminal V spinal sensory nucleus	Moderate
TS28: caudate-putamen	Weak
TS28: cerebellum internal granule cell layer	Weak
TS28: deep cerebellar nucleus	Weak
TS28: dorsal thalamus	Weak
TS28: lateral geniculate nucleus	Weak
TS28: main olfactory bulb glomerular layer	Weak
TS28: main olfactory bulb granule cell layer	Weak
TS28: reticular thalamic nucleus	Weak
TS28: hypothalamic nucleus	Weak	Regionally restricted	Expression is in the ventral nuclei and in the estria terminalis nuclei.
TS28: anterior ventral thalamic nucleus	Trace
TS28: inferior olivary nucleus	Trace
TS28: medial vestibular nucleus	Trace
TS28: prethalamus	Trace
TS28: spinal vestibular nucleus	Trace
TS28: subthalamic nucleus	Trace
TS28: brain	Trace	Regionally restricted	Expression is in the lemniscus.
TS28: habenula	Absent
TS28: inferior colliculus	Absent
TS28: midbrain tegmentum	Absent
TS28: pretectum	Absent

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