Antibody:	Anti-Ncoa4 (Q19) sc-30968
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle cardiac muscle	Present		4B E9.5

Structure	Level	Pattern	Image	Note
TS17: primitive ventricle cardiac muscle	Present		4B E10.5

Structure	Level	Pattern	Image	Note
TS19: ventricle cardiac muscle	Present		4B E11.5

Structure	Level	Pattern	Image	Note
TS20: ventricle cardiac muscle	Present		4B E12.5

Structure	Level	Pattern	Image	Note
TS22: ventricle cardiac muscle	Present		4B E14.5

Structure	Level	Pattern	Image	Note
TS23: ventricle cardiac muscle	Present		4B E15.5

Structure	Level	Pattern	Image	Note
TS24: ventricle cardiac muscle	Present		4B E16.5

Structure	Level	Pattern	Image	Note
TS25: ventricle cardiac muscle	Present		4B E17.5

Structure	Level	Pattern	Image	Note
TS17: liver	Present		5B E10.5

Structure	Level	Pattern	Image	Note
TS19: liver	Present		5B E11.5

Structure	Level	Pattern	Image	Note
TS20: liver	Strong		5B E12.5

Structure	Level	Pattern	Image	Note
TS22: liver	Present		5B E14.5

Structure	Level	Pattern	Image	Note
TS23: liver	Present		5B E15.5

Structure	Level	Pattern	Image	Note
TS24: liver	Strong		5B E16.5

Structure	Level	Pattern	Image	Note
TS25: liver	Strong		5B E17.5

Structure	Level	Pattern	Image	Note
TS19: lung	Ambiguous		6B E11.5

Structure	Level	Pattern	Image	Note
TS20: lung	Strong	Regionally restricted	6B E12.5	High levels of staining were observed in the bronchioles.

Structure	Level	Pattern	Image	Note
TS22: lung	Strong	Regionally restricted	6B E14.5	High levels of staining were observed in the bronchioles.

Structure	Level	Pattern	Image	Note
TS23: lung	Trace	Regionally restricted	6B E15.5	Staining was almost undetectable in the bronchioles.

Structure	Level	Pattern	Image	Note
TS25: bronchiole	Trace		6B E17.5

Structure	Level	Pattern	Image	Note
TS28: ovary	Present		6C Ovary

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatid		Strong		9_Testis
TS28: spermatocyte		Strong		9_Testis
TS28: spermatogonium		Strong		9_Testis
TS28: Sertoli cell	Sertoli cell	Strong		9_Testis
TS28: Leydig cell	Leydig cell	Weak		9_Testis

Structure	Level	Pattern	Image	Note
TS28: prostate epithelium	Present		9_Prostate
TS28: prostate stroma	Absent		9_Prostate

Structure	Level	Pattern	Image	Note
TS28: epithelium of epididymis	Strong	Regionally restricted	9_Epididymis	Expression was detected in the columnar epithelial cells.
TS28: muscle layer of epididymis	Absent		9_Epididymis

Structure	Level	Pattern	Image	Note
TS28: epithelium of seminal vesicle	Present	Regionally restricted	9_Seminal Vesicles	Expression was detected in the columnar epithelial cells.
TS28: muscle of seminal vesicle	Absent		9_Seminal Vesicles

Structure	Level	Pattern	Image	Note
TS28: oocyte	Strong		9_Ovary
TS28: ovary theca	Strong		9_Ovary
TS28: ovary follicle	Strong	Regionally restricted	9_Ovary	Expression was detected in the ovarian granulosa cells.
TS28: mesenchymal stroma of ovary	Absent		9_Ovary

Structure	Level	Pattern	Image	Note
TS28: endometrium	Strong	Uniform	9_Uterus
TS28: uterine gland	Strong		9_Uterus
TS28: myometrium	Absent		9_Uterus

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Present		9_Heart	Staining in the adult was less intense than during development.

Structure	Level	Pattern	Image	Note
TS28: alveolus epithelium	Present		9_Lung
TS28: bronchiole epithelium	Present		9_Lung
TS28: bronchiole smooth muscle	Absent		9_Lung

Structure	Level	Pattern	Image	Note
TS28: liver	Weak		9_Liver

Structure	Level	Pattern	Image	Note
TS28: spleen	Strong		9_Spleen
TS28: spleen red pulp	Strong		9_Spleen
TS28: spleen white pulp	Strong		9_Spleen

Structure	Level	Pattern	Image	Note
TS28: renal distal tubule	Strong		9_Kidney
TS28: renal proximal tubule	Present		9_Kidney

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Present		9_Skeletal Muscle	Expression was detected in skeletal muscle fibers, whereas the nuclei at the periphery of the fibers were negative.