Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:111609
Anderson RM, et al., Endogenous bone morphogenetic protein antagonists regulate mammalian neural crest generation and survival. Dev Dyn. 2006 Aug 7;235(9):2507-2520
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5584017
|
|
Gene symbol:
|
Tfap2a
|
|
Gene name:
|
transcription factor AP-2, alpha
|
|
Antibody:
|
clone 3B5
|
|
Detection system:
|
Secondary antibody coupled to Cy3
|
|
|
Results
|
Specimen
8A:
embryonic day 8.5
(more  )
Note: 11- 12 somites. A'-C': Higher magnification of corresponding boxed areas in A-C. Triple labeled: red - Tfap2a; green - TUNEL; blue - phospho-Histone H3 - blue.
Specimen
8A:
(close  )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
11- 12 somites. A'-C': Higher magnification of corresponding boxed areas in A-C. Triple labeled: red - Tfap2a; green - TUNEL; blue - phospho-Histone H3 - blue.
|
Specimen
8B:
embryonic day 8.5; Nogtm1Amc/ Nogtm1Amc
(more )
Note: 11- 12 somites. A'-C': Higher magnification of corresponding boxed areas in A-C. Triple labeled: red - Tfap2a; green - TUNEL; blue - phospho-Histone H3 - blue.
Specimen
8B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv
|
|
Mutant Allele(s):
|
Nogtm1Amc/Nogtm1Amc
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
11- 12 somites. A'-C': Higher magnification of corresponding boxed areas in A-C. Triple labeled: red - Tfap2a; green - TUNEL; blue - phospho-Histone H3 - blue.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural crest |
Present |
|
8B
,
8B'
|
At the level of the otic placodes/second branchial arch, the amount of positive neural crest cells has increased in this mutant.
|
Specimen
8C:
embryonic day 8.5; Chrdtm1Emdr/ Chrdtm1Emdr, Nogtm1Amc/ Nogtm1Amc
(more )
Note: 11- 12 somites. A'-C': Higher magnification of corresponding boxed areas in A-C. Triple labeled: red - Tfap2a; green - TUNEL; blue - phospho-Histone H3 - blue.
Specimen
8C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Chrdtm1Emdr/Chrdtm1Emdr
Nogtm1Amc/Nogtm1Amc
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
11- 12 somites. A'-C': Higher magnification of corresponding boxed areas in A-C. Triple labeled: red - Tfap2a; green - TUNEL; blue - phospho-Histone H3 - blue.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural crest |
Strong |
|
8C'
,
8C
|
At the level of the otic placodes/second branchial arch, the amount of positive neural crest cells has increased in this mutant.
|
|
Analysis Tools