Reporter:	lacZ
	Direct Detection
Assay notes:	Embryos were fixed in 0.2% glutaraldehyde and post-fixed in 4% paraformaldehyde. Sections are vibratome sections of whole mount stained specimens.

Structure	Level	Pattern	Image	Note
TS11: allantois	Strong		1A
TS11: embryo ectoderm	Strong		1A
TS11: embryo mesoderm	Strong		1A
TS11: extraembryonic ectoderm	Strong		1A
TS11: amnion ectoderm	Strong	Regionally restricted	1A	Expression is in the posterior amniotic fold.
TS11: ectoplacental cone	Absent		1A
TS11: embryo endoderm	Absent		1A
TS11: extraembryonic endoderm	Absent		1A

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Absent		1B

Structure	Level	Pattern	Image	Note
TS11: allantois	Strong		1C
TS11: extraembryonic ectoderm	Strong		1C
TS11: amnion ectoderm	Strong	Regionally restricted	1C	Expression is in the posterior amniotic fold.
TS11: extraembryonic endoderm	Absent		1C

Structure	Level	Pattern	Image	Note
TS11: allantois	Strong		1D
TS11: extraembryonic ectoderm	Strong		1D
TS11: amnion ectoderm	Strong	Regionally restricted	1D	Expression is in the posterior amniotic fold.
TS11: extraembryonic endoderm	Absent		1D

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Strong		1E
TS11: embryo mesoderm	Strong		1E
TS11: embryo endoderm	Absent		1E

Structure	Level	Pattern	Image	Note
TS12: paraxial mesenchyme	Strong		2A
TS12: neural ectoderm	Present		2A

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present		2C

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present		2D
TS12: neural ectoderm	Present		2D
TS12: 1st branchial pouch	Present	Regionally restricted	2D	Expression is in the epithelium.

Structure	Level	Pattern	Image	Note
TS12: cardiac muscle tissue	Present		2E
TS12: neural ectoderm	Present		2E

Structure	Level	Pattern	Image	Note
TS12: paraxial mesenchyme	Strong		2F

Structure	Level	Pattern	Image	Note
TS13: neural tube	Strong		3A
TS13: paraxial mesenchyme	Strong		3A
TS13: future hindbrain	Present		3A
TS13: future midbrain	Present		3A
TS13: future prosencephalon	Present		3A
TS13: somite	Present	Regionally restricted	3A	Expression is in the dermomyotome.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present		3B
TS13: future midbrain	Present		3B
TS13: future prosencephalon	Present		3B
TS13: neural tube	Present		3B
TS13: somite	Present	Regionally restricted	3B	Expression is in the dermomyotome.

Structure	Level	Pattern	Image	Note
TS13: paraxial mesenchyme	Strong		3C
TS13: future hindbrain	Present		3C
TS13: future midbrain	Present		3C

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch ectoderm	Present		3G
TS13: neural ectoderm	Present		3G

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present		3H
TS13: heart	Present		3H
TS13: neural tube	Present		3H

Structure	Level	Pattern	Image	Note
TS13: hindgut diverticulum	Present		3I

Structure	Level	Pattern	Image	Note
TS13: somite	Present	Regionally restricted	3J	Expression is in the dermomyotome.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Strong		4A
TS15: forelimb bud mesenchyme	Present	Diffuse	4A
TS15: 1st branchial arch	Present		4A
TS15: 2nd branchial arch	Present		4A
TS15: dermomyotome	Present		4A
TS15: future midbrain	Present		4A
TS15: otocyst epithelium	Present		4A
TS15: future forebrain	Present	Regionally restricted	4A	Expression is in the central parts.
TS15: future hindbrain	Present	Regionally restricted	4A	Expression is in the central parts.
TS15: optic vesicle	Present	Regionally restricted	4A	Expression is in the epithelium.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Strong		4B
TS15: forelimb bud mesenchyme	Present	Diffuse	4B
TS15: future midbrain	Present		4B
TS15: otocyst epithelium	Present		4B
TS15: future forebrain	Present	Regionally restricted	4B	Expression is in the central parts.
TS15: future hindbrain	Present	Regionally restricted	4B	Expression is in the central parts.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Strong		4C
TS15: forelimb bud mesenchyme	Present	Diffuse	4C
TS15: neural tube	Present		4C
TS15: future forebrain	Present	Regionally restricted	4C	Expression is in the central parts.
TS15: future hindbrain	Present	Regionally restricted	4C	Expression is in the central parts.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		4D
TS15: future forebrain	Present	Regionally restricted	4D	Expression is in the central parts.
TS15: future hindbrain	Present	Regionally restricted	4D	Expression is in the central parts.
TS15: head mesenchyme	Absent		4D

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	4E	Expression is in the central parts.
TS15: future hindbrain	Present	Regionally restricted	4E	Expression is in the central parts.

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Present		4F

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present		4G
TS15: 2nd branchial arch	Present		4G
TS15: neural tube	Present		4G
TS15: future forebrain	Present	Regionally restricted	4G	Expression is in the central parts.
TS15: future hindbrain	Present	Regionally restricted	4G	Expression is in the central parts.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		4H
TS15: thyroid primordium	Present		4H

Structure	Level	Pattern	Image	Note
TS15: gut epithelium	Present		4I
TS15: notochord	Present		4I

Structure	Level	Pattern	Image	Note
TS15: common atrial chamber cardiac muscle	Present		4J
TS15: gut epithelium	Present		4J
TS15: mesonephros	Present		4J
TS15: neural tube	Present		4J
TS15: notochord	Present		4J
TS15: primitive ventricle	Present		4J

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Strong		4K
TS15: forelimb bud mesenchyme	Present	Diffuse	4K
TS15: dermomyotome	Present		4K

Structure	Level	Pattern	Image	Note
TS17: dermomyotome	Present		5A
TS17: forebrain	Present		5A
TS17: hindbrain	Present		5A
TS17: midbrain	Present		5A
TS17: tail unsegmented mesenchyme	Present		5A
TS17: 1st branchial arch	Present	Regionally restricted	5A	Expression is in the invaginating folds and leading edges.
TS17: 2nd branchial arch	Present	Regionally restricted	5A	Expression is in the invaginating folds and leading edges.
TS17: 3rd branchial arch	Present	Regionally restricted	5A	Expression is in the invaginating folds and leading edges.
TS17: heart	Strong	Regionally restricted	5A	Expression is in the aortic sac.
TS17: common atrial chamber cardiac muscle	Strong		5A
TS17: forelimb bud ectoderm	Strong		5A
TS17: forelimb bud mesenchyme	Strong		5A
TS17: hindlimb bud ectoderm	Strong		5A
TS17: hindlimb bud mesenchyme	Strong		5A
TS17: primitive ventricle	Strong	Regionally restricted	5A	Expression is in the trabeculation.
TS17: atrioventricular canal	Absent		5A

Structure	Level	Pattern	Image	Note
TS17: forelimb bud ectoderm	Strong		5B
TS17: forelimb bud mesenchyme	Strong		5B
TS17: neural tube	Strong		5B
TS17: forebrain	Present		5B
TS17: hindbrain	Present		5B
TS17: midbrain	Present		5B
TS17: ear	Present	Regionally restricted	5B	Expression is medio-dorsal at the outgrowing edges.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		5C
TS17: hindbrain	Present		5C
TS17: head mesenchyme	Absent		5C

Structure	Level	Pattern	Image	Note
TS17: optic cup inner layer	Strong		5D
TS17: lens pit	Weak		5D
TS17: optic cup outer layer	Absent		5D
TS17: optic stalk	Absent		5D

Structure	Level	Pattern	Image	Note
TS17: ear	Present	Regionally restricted	5E	Expression is medio-dorsal at the outgrowing edges.
TS17: 1st branchial arch	Present	Regionally restricted	5E	Expression is in the invaginating folds and leading edges.
TS17: 2nd branchial arch	Present	Regionally restricted	5E	Expression is in the invaginating folds and leading edges.
TS17: 3rd branchial arch	Present	Regionally restricted	5E	Expression is in the invaginating folds and leading edges.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud ectoderm	Strong		5F
TS17: forelimb bud mesenchyme	Strong		5F
TS17: mesonephric tubule	Strong		5F
TS17: nephric duct, mesonephric portion	Strong		5F
TS17: neural tube	Strong		5F

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong		5G
TS17: dermomyotome	Present		5G
TS17: tail unsegmented mesenchyme	Present		5G

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		5H
TS17: hindbrain	Present		5H
TS17: midbrain	Present		5H

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present	Regionally restricted	5I	Expression is in the invaginating folds and leading edges.
TS17: 2nd branchial arch	Present	Regionally restricted	5I	Expression is in the invaginating folds and leading edges.
TS17: 3rd branchial arch	Present	Regionally restricted	5I	Expression is in the invaginating folds and leading edges.

Structure	Level	Pattern	Image	Note
TS17: thyroid primordium	Present		5J

Structure	Level	Pattern	Image	Note
TS17: forelimb bud ectoderm	Strong		5K
TS17: forelimb bud mesenchyme	Strong		5K
TS17: neural tube	Strong		5K
TS17: notochord	Strong		5K
TS17: lung	Moderate		5K
TS17: esophagus	Present		5K

Structure	Level	Pattern	Image	Note
TS17: heart	Strong	Regionally restricted	5L	Expression is in the aortic sac.
TS17: common atrial chamber cardiac muscle	Strong		5L
TS17: primitive ventricle	Strong	Regionally restricted	5L	Expression is in the trabeculation.
TS17: atrioventricular canal	Absent		5L

Structure	Level	Pattern	Image	Note
TS17: mesonephric tubule	Strong		5M
TS17: nephric duct, mesonephric portion	Strong		5M
TS17: neural tube	Strong		5M
TS17: hindgut	Present		5M

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present	Regionally restricted	5N	Expression is in the invaginating folds and leading edges.
TS17: 2nd branchial arch	Present	Regionally restricted	5N	Expression is in the invaginating folds and leading edges.
TS17: hindgut	Present		5N

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present		6A
TS19: neural tube	Present		6A
TS19: somite	Present		6A
TS19: ear	Present	Regionally restricted	6A	Expression is in the medio-dorsal epithelial portion.
TS19: nose	Present	Regionally restricted	6A	Expression is in the epithelium of the forming folds and leading edges.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		6B
TS19: telencephalon	Present		6B

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present		6C
TS19: telencephalon	Present		6C
TS19: midbrain-hindbrain junction	Strong		6C
TS19: stomach	Strong		6C
TS19: ear	Present	Regionally restricted	6C	Expression is in the medio-dorsal epithelial portion.
TS19: nose	Present	Regionally restricted	6C	Expression is in the epithelium of the forming folds and leading edges.
TS19: liver	Present	Regionally restricted	6C	Expression is in the capsule, interlobular septums, and in distinct cells of some areas at the peripheral part of the liver.
TS19: liver parenchyma	Absent		6C

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present		6D
TS19: midbrain-hindbrain junction	Strong		6D

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present		6E
TS19: diencephalon	Strong	Regionally restricted	6E	Expression is in the hypothalamus.
TS19: midbrain-hindbrain junction	Strong		6E

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	6F	Expression is in the choroid plexus.

Structure	Level	Pattern	Image	Note
TS19: future pituitary	Present		6G
TS19: neural retinal epithelium	Strong		6G
TS19: optic stalk	Strong	Regionally restricted	6G	Expression is in the optic nerve.
TS19: cornea epithelium	Trace		6G
TS19: lens vesicle	Trace		6G
TS19: nose	Present	Regionally restricted	6G	Expression is in the epithelium of the forming folds and leading edges.

Structure	Level	Pattern	Image	Note
TS19: left atrium cardiac muscle	Strong		6H
TS19: right atrium cardiac muscle	Strong		6H
TS19: septum primum	Strong		6H
TS19: left ventricle cardiac muscle	Strong	Regionally restricted	6H	Expression is in the trabeculations.
TS19: right ventricle cardiac muscle	Strong	Regionally restricted	6H	Expression is in the trabeculations.
TS19: atrioventricular canal	Absent		6H
TS19: endocardial cushion	Absent		6H
TS19: interventricular septum	Absent		6H

Structure	Level	Pattern	Image	Note
TS19: left atrium cardiac muscle	Present		6I
TS19: right atrium cardiac muscle	Present		6I
TS19: septum primum	Present		6I
TS19: lung mesenchyme	Present	Regionally restricted	6I	Expression is distal.
TS19: left ventricle cardiac muscle	Present	Regionally restricted	6I	Expression is in the trabeculations.
TS19: right ventricle cardiac muscle	Present	Regionally restricted	6I	Expression is in the trabeculations.
TS19: intraembryonic coelom	Strong	Regionally restricted	6I	Expression is in the diaphragm.
TS19: liver	Present	Regionally restricted	6I	Expression is in the capsule, interlobular septums, and in distinct cells of some areas at the peripheral part of the liver.
TS19: liver parenchyma	Absent		6I

Structure	Level	Pattern	Image	Note
TS19: stomach	Strong		6J
TS19: intraembryonic coelom	Strong	Regionally restricted	6J	Expression is in the diaphragm.
TS19: liver	Present	Regionally restricted	6J	Expression is in the capsule, interlobular septums, and in distinct cells of some areas at the peripheral part of the liver.
TS19: liver parenchyma	Absent		6J

Structure	Level	Pattern	Image	Note
TS19: hindgut epithelium	Strong		6K
TS19: hindgut	Present		6K
TS19: midgut	Present		6K

Structure	Level	Pattern	Image	Note
TS19: notochord	Present		6L
TS19: genital tubercle	Very strong		6L

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Very strong		6M

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Very strong	Regionally restricted	6N	Expression is in the elbow joint primordium and in the perichondrium of the long bones.
TS19: handplate	Very strong	Regionally restricted	6N	Expression is at the tip.

Structure	Level	Pattern	Image	Note
TS19: tail somite	Present		6O
TS19: tail	Very strong	Regionally restricted	6O	Expression is at the end.

Structure	Level	Pattern	Image	Note
TS19: infundibular recess of 3rd ventricle	Present		7A
TS19: myelencephalon	Present		7A
TS19: telencephalon	Present		7A
TS19: diencephalon	Present	Regionally restricted	7A	Expression is in the epithalamus and thalamus and is strong in the hypothalamus.
TS19: metencephalon basal plate	Present	Regionally restricted	7A	Expression is in the pons.
TS19: trigeminal ganglion	Weak		7A
TS19: cranial ganglion	Weak	Regionally restricted	7A	Expression is in the vestibulocochlear ganglion.
TS19: ear	Present	Regionally restricted	7A	Expression is in the medio-dorsal epithelial portion.

Structure	Level	Pattern	Image	Note
TS19: mesonephros	Strong	Regionally restricted	7B	Expression is in the mesonephric ridge.
TS19: hindgut epithelium	Strong		7B
TS19: notochord	Present		7B
TS19: liver	Present	Regionally restricted	7B	Expression is in the capsule, interlobular septums, and in distinct cells of some areas at the peripheral part of the liver.
TS19: myotome	Trace		7B	Expression is in pre-muscle masses.
TS19: liver parenchyma	Absent		7B

Structure	Level	Pattern	Image	Note
TS19: brain floor plate	Present		7C
TS19: hindbrain roof plate	Present		7C

Structure	Level	Pattern	Image	Note
TS19: infundibular recess of 3rd ventricle	Present		7D
TS19: trigeminal ganglion	Weak		7D
TS19: cranial ganglion	Weak	Regionally restricted	7D	Expression is in the vestibulocochlear ganglion.
TS19: ear	Present	Regionally restricted	7D	Expression is in the medio-dorsal epithelial portion.

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Strong		7E
TS19: optic stalk	Strong	Regionally restricted	7E	Expression is in the optic nerve.
TS19: cornea epithelium	Trace		7E
TS19: lens vesicle	Trace		7E
TS19: eye	Present	Regionally restricted	7E	Expression is in the epithelium of the leading edges of the invaginating folds in the eyelids.

Structure	Level	Pattern	Image	Note
TS19: nose	Present	Regionally restricted	7F	Expression is in the epithelium of the forming folds and leading edges and in Jacobson's organ.
TS19: olfactory epithelium	Present	Regionally restricted	7F	Expression is in the epithelium of the forming folds and leading edges and in Jacobson's organ.
TS19: oral epithelium	Present	Regionally restricted	7F	Expression is in the epithelium of the forming folds and leading edges and in Jacobson's organ.
TS19: nasal process mesenchyme	Present	Diffuse	7F
TS19: oral region	Present	Diffuse	7F

Structure	Level	Pattern	Image	Note
TS19: esophagus	Strong		7G
TS19: left atrium cardiac muscle	Strong		7G
TS19: right atrium cardiac muscle	Strong		7G
TS19: septum primum	Strong		7G
TS19: left ventricle cardiac muscle	Strong	Regionally restricted	7G	Expression is in the trabeculations.
TS19: right ventricle cardiac muscle	Strong	Regionally restricted	7G	Expression is in the trabeculations.
TS19: trachea	Present	Regionally restricted	7G	Expression is ventral.
TS19: atrioventricular canal	Absent		7G
TS19: endocardial cushion	Absent		7G
TS19: interventricular septum	Absent		7G

Structure	Level	Pattern	Image	Note
TS19: esophagus	Strong		7H
TS19: lung mesenchyme	Strong	Regionally restricted	7H	Expression is distal.

Structure	Level	Pattern	Image	Note
TS19: mesonephros	Strong	Regionally restricted	7I	Expression is in the mesonephric ridge.
TS19: lung mesenchyme	Present	Regionally restricted	7I	Expression is distal.
TS19: liver	Present	Regionally restricted	7I	Expression is in the capsule, interlobular septums, and in distinct cells of some areas at the peripheral part of the liver.
TS19: liver parenchyma	Absent		7I

Structure	Level	Pattern	Image	Note
TS19: vagus ganglion	Very strong		7J
TS19: neural tube mantle layer	Present		7J
TS19: notochord	Present		7J
TS19: neural tube	Present	Regionally restricted	7J	Expression is in the ependymal layer.

Structure	Level	Pattern	Image	Note
TS19: gonad	Very strong		7K

Structure	Level	Pattern	Image	Note
TS19: hindgut epithelium	Strong		7L
TS19: stomach	Strong		7L
TS19: mesonephros	Strong	Regionally restricted	7L	Expression is in the mesonephric ridge.
TS19: hindgut	Present		7L
TS19: midgut	Present		7L

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Very strong		7M

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Very strong	Regionally restricted	7N	Expression is in the elbow joint primordium and in the perichondrium of the long bones.
TS19: handplate	Very strong	Regionally restricted	7N	Expression is at the tip.

Structure	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	Present			Expression is almost identical as described for E13.5.
TS20: choroid plexus	Present			Expression is almost identical as described for E13.5.
TS20: corpus striatum	Present			Expression is almost identical as described for E13.5.
TS20: cranial ganglion	Present			Expression is almost identical as described for E13.5.
TS20: diencephalon lamina terminalis	Present			Expression is almost identical as described for E13.5.
TS20: dorsal root ganglion	Present			Expression is almost identical as described for E13.5.
TS20: ear	Present			Expression is almost identical as described for E13.5.
TS20: eye	Present			Expression is almost identical as described for E13.5.
TS20: jaw primordium	Present			Expression is almost identical as described for E13.5.
TS20: limb mesenchyme	Present			Expression is almost identical as described for E13.5.
TS20: liver	Present			Expression is almost identical as described for E13.5.
TS20: lobar bronchus	Present			Expression is almost identical as described for E13.5.
TS20: medulla oblongata	Present			Expression is almost identical as described for E13.5.
TS20: metanephros	Present			Expression is almost identical as described for E13.5.
TS20: midbrain-hindbrain junction	Present			Expression is almost identical as described for E13.5.
TS20: midgut	Present			Expression is almost identical as described for E13.5.
TS20: nose	Present			Expression is almost identical as described for E13.5.
TS20: palatal shelf	Present			Expression is almost identical as described for E13.5.
TS20: pinna	Present			Expression is almost identical as described for E13.5.
TS20: skeletal muscle	Present			Expression is almost identical as described for E13.5.
TS20: spinal cord	Present			Expression is almost identical as described for E13.5.
TS20: tail	Present			Expression is almost identical as described for E13.5.
TS20: telencephalon	Present			Expression is almost identical as described for E13.5.
TS20: vibrissa	Present			Expression is almost identical as described for E13.5.
TS20: vomeronasal organ	Present			Expression is almost identical as described for E13.5.

Structure	Level	Pattern	Image	Note
TS21: pinna	Very strong		8A
TS21: vibrissa	Very strong		8A
TS21: telencephalon	Present	Regionally restricted	8A	Expression is in the frontal portions.

Structure	Level	Pattern	Image	Note
TS21: tail	Present	Regionally restricted	8B	Expression is at the distal tip.

Structure	Level	Pattern	Image	Note
TS21: pinna	Very strong		8C
TS21: spinal cord	Weak		8C

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	8D	Expression is in the frontal portions.
TS21: corpus striatum	Present		8D
TS21: medulla oblongata	Weak		8D

Structure	Level	Pattern	Image	Note
TS21: pinna	Very strong		8E
TS21: jaw	Present	Regionally restricted	8E	Expression is in the epitelium of the outgrowing and invaginating edges.
TS21: nose	Present	Regionally restricted	8E	Expression is in the epitelium of the outgrowing and invaginating edges.
TS21: palatal shelf	Present	Regionally restricted	8E	Expression is in the epitelium of the outgrowing and invaginating edges.
TS21: vomeronasal organ	Present		8E
TS21: tooth epithelium	Present	Regionally restricted	8E	Expression is in the dental lamina.
TS21: cranial ganglion	Weak		8E
TS21: spinal cord	Weak		8E
TS21: tongue	Absent		8E

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Strong		8F
TS21: skeletal muscle	Present		8F
TS21: femur cartilage condensation	Present	Regionally restricted	8F	Expression is in the perichondrium and is absent in the cartilage itself.
TS21: rib cartilage condensation	Present	Regionally restricted	8F	Expression is in the perichondrium and is absent in the cartilage itself.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	8G	Expression is in the frontal portions.
TS21: diencephalon lamina terminalis	Present		8G

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Strong	Regionally restricted	8H	Expression is at the border of the hyaloid cavity.
TS21: eyelid	Strong	Regionally restricted	8H	Expression is at the leading edges.
TS21: cornea epithelium	Weak		8H
TS21: lens	Weak		8H

Structure	Level	Pattern	Image	Note
TS21: trigeminal ganglion	Weak		8I
TS21: ear	Present	Regionally restricted	8I	Expression is in the medio-dorsal part near the vestibulochoclear ganglion.

Structure	Level	Pattern	Image	Note
TS21: pinna	Very strong		8J
TS21: skeletal muscle	Present		8J

Structure	Level	Pattern	Image	Note
TS21: jaw	Present		8K	Expression is in the epitelium of the outgrowing and invaginating edges.
TS21: nasal cavity epithelium	Present		8K	Expression is in the epitelium of the outgrowing and invaginating edges.
TS21: oral epithelium	Present		8K	Expression is in the epitelium of the outgrowing and invaginating edges.
TS21: palatal shelf	Present		8K	Expression is in the epitelium of the outgrowing and invaginating edges.
TS21: posterior naris epithelium	Present		8K	Expression is in the epitelium of the outgrowing and invaginating edges.
TS21: nasal mesenchyme	Present	Diffuse	8K
TS21: oral region	Present	Diffuse	8K	Expression is diffuse in the mesenchyme.
TS21: vomeronasal organ	Present		8K
TS21: tooth epithelium	Present	Regionally restricted	8K	Expression is in the dental lamina.
TS21: tongue	Absent		8K

Structure	Level	Pattern	Image	Note
TS21: lateral ventricle choroid plexus	Present		8L

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		8M

Structure	Level	Pattern	Image	Note
TS21: lobar bronchus mesenchyme	Present	Regionally restricted	8N	Expression is in the outgrowing edges of the new segments.

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Strong		8O
TS21: interventricular septum	Absent		8O

Structure	Level	Pattern	Image	Note
TS21: liver	Present	Regionally restricted	8P	Expression is in the septum, capsule, and in some distinct cells in the peripheral part.

Structure	Level	Pattern	Image	Note
TS21: liver	Present	Regionally restricted	8Q	Expression is in the septum, capsule, and in some distinct cells in the peripheral part.
TS21: midgut loop	Strong	Regionally restricted	8Q	Expression is in the physiological hernia.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Strong		8R
TS21: metanephros	Strong	Regionally restricted	8R	Expression is stronger in the distal outgrowing edges.
TS21: skeletal muscle	Present		8R
TS21: spinal cord mantle layer	Weak		8R

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		8S
TS21: midbrain-hindbrain junction	Present		8S

Structure	Level	Pattern	Image	Note
TS21: vibrissa	Very strong		8T
TS21: neural retinal epithelium	Strong	Regionally restricted	8T	Expression is at the border of the hyaloid cavity.
TS21: eyelid	Strong	Regionally restricted	8T	Expression is at the leading edges.
TS21: cornea epithelium	Weak		8T
TS21: lens	Weak		8T

Structure	Level	Pattern	Image	Note
TS21: forelimb digit pre-cartilage condensation	Present	Regionally restricted	8U	Expression is in the perichondrium and is absent in the cartilage itself.
TS21: radius-ulna cartilage condensation	Present	Regionally restricted	8U	Expression is in the perichondrium and is absent in the cartilage itself.

Structure	Level	Pattern	Image	Note
TS21: tibia-fibula pre-cartilage condensation	Present	Regionally restricted	8V	Expression is in the perichondrium and is absent in the cartilage itself.