Probe:	MTGR1 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	1A	Expressed in the dorsal telencephalon. Expression was highly enriched in the preplate.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2A	Expressed in the dorsal telencephalon. More broadly expressed across the cortical wall. Very weak expression in the ventral telencephalon, including the lateral ganglionic eminences (LGE).

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2B	Expressed in the dorsal telencephalon. More broadly expressed across the cortical wall. Very weak expression in the ventral telencephalon, including caudal ganglionic eminences (CGE).
TS19: diencephalon	Strong	Regionally restricted	2B	Expressed in the caudal diencephalon, including the thalamus. Expressed in the eminentia thalami.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2C	Expressed in the dorsal telencephalon. More broadly expressed across the cortical wall.
TS19: diencephalon	Strong	Regionally restricted	2C	Expressed in the caudal diencephalon, including the thalamus. Weak expression in the prethalamus.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Strong	Regionally restricted	2D	Expressed in the rostral pretectum.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	3A	Expressed in the dorsal telencephalon with a clear laminar preference.
TS21: cerebral cortex subventricular zone	Strong		3A
TS21: cortical preplate	Strong		3A
TS21: cerebral cortex ventricular layer	Present	Scattered	3A

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	3B	Expressed in the dorsal telencephalon with a clear laminar preference.
TS21: cerebral cortex subventricular zone	Strong		3B
TS21: cortical preplate	Strong		3B
TS21: cerebral cortex ventricular layer	Present	Scattered	3B

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	3C , 3D	Expressed in the dorsal telencephalon with a clear laminar preference.
TS21: cerebral cortex subventricular zone	Strong		3D , 3C
TS21: cortical preplate	Strong		3D , 3C
TS21: cerebral cortex ventricular layer	Present	Scattered	3C , 3D
TS21: thalamus	Present		3C
TS21: diencephalon	Present	Regionally restricted	3C	Expressed in the prethalamus, eminentia thalami and thalamus.

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Regionally restricted	4A	Expression was down-regulated. Expressed in the lateral part of the dorsal telencephalon.

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Regionally restricted	4B	Expression was down-regulated. Expressed in the medial and lateral parts of the dorsal telencephalon.
TS23: diencephalon	Present	Regionally restricted	4B	Down-regulated expression along the outer edge of the ventricular zone in the pretectum, in the prethalamus and thalamus.

Structure	Level	Pattern	Image	Note
TS19: retina	Strong	Regionally restricted	5C	Expressed in the central part.

Structure	Level	Pattern	Image	Note
TS21: retina	Strong	Regionally restricted	6A	Robust expression in the ventricular zone. Also expressed in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS23: retina	Present		6B	Expression was further enriched in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	7A	Expressed broadly in the middle layer.
TS20: hindbrain	Present	Regionally restricted	7A	Expressed in the dorsal hindbrain near the 4th ventricle.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	7B	Expressed broadly in the middle layer.
TS20: hindbrain	Present	Regionally restricted	7B	Expressed in the dorsal hindbrain near the 4th ventricle.
TS20: cerebellum primordium	Strong		7B

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	8A	Expressed in the most dorsal progenitor population. Also expressed more ventrally.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Widespread	8B	Expression became broader, covering most of the dorso-ventral extent, but with weaker expression ventrally.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Regionally restricted		Expressed in the medial and lateral parts of the dorsal telencephalon.
TS27: retina	Present			The expression level appeared to be decreased.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted		Expressed along the outer edge of the ventricular zone in the pretectum.