Gal RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:141520 Schweickert A, et al., Left-asymmetric expression of Galanin in the linear heart tube of the mouse embryo is independent of the nodal co-receptor gene cryptic. Dev Dyn. 2008 Dec;237(12):3557-64
Assay type:	RNA in situ
MGI Accession ID:	MGI:5569276
Gene symbol:	Gal
Gene name:	galanin and GMAP prepropeptide

Probe:	Gal cDNA
Probe preparation:	Antisense RNA

Results

1A left ©

Specimen 1A left: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		1A left
TS11: notochordal plate	Weak	Regionally restricted	1A left	Expressed in the posterior notochord.

1A right ©

Specimen 1A right: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Widespread	1A right	Expressed in all three germ layers.
TS11: notochordal plate	Weak	Regionally restricted	1A right	Expressed in the posterior notochord.

1B ©

Specimen 1B: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Absent		1B
TS12: notochordal plate	Weak	Regionally restricted	1B	Expressed in crown cells lining the posterior notochord.

1C left ©

Specimen 1C left: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: cardiac muscle tissue	Present	Regionally restricted	1C left	Expressed in the left linear heart, with stronger signals in the dorsal part.
TS13: notochordal plate	Absent		1C left

1C right ©

Specimen 1C right: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: cardiac muscle tissue	Present	Regionally restricted	1C right	Expressed in the myocard of the left heart tube.

1D left ©

Specimen 1D left: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Present	Regionally restricted	1D left	Expressed in a ring-like pattern.

1D right ©

Specimen 1D right: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Present	Regionally restricted	1D right	Expressed in a ring-like pattern.

1E left ©

Specimen 1E left: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Present	Regionally restricted	1E left	Expressed in a ring-like pattern, with stronger signal intensities at the dorsal and ventral sides.

1E right ©

Specimen 1E right: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Present	Regionally restricted	1E right	Expressed in a ring-like pattern, with stronger signal intensities at the dorsal and ventral sides.

1F upper ©

Specimen 1F upper: embryonic day 13.0 (more

)

Structure	Level	Pattern	Image	Note
TS21: atrioventricular canal	Present	Regionally restricted	1F upper	Expressed in the secondary pace maker, the atrio-ventricular node and ring.
TS21: heart left ventricle	Weak		1F upper
TS21: heart right ventricle	Weak		1F upper

1F lower ©

Specimen 1F lower: embryonic day 13.0 (more

)

Structure	Level	Pattern	Image	Note
TS21: atrioventricular canal	Present	Regionally restricted	1F lower	Expressed in the secondary pace maker, the atrio-ventricular node and ring.

1H ©

Specimen 1H: embryonic day 13.0 (more

)

Structure	Level	Pattern	Image	Note
TS21: atrioventricular canal	Present	Regionally restricted	1H	Expressed in the secondary pace maker, the atrio-ventricular ring.

2A ©

Specimen 2A: embryonic day 8.0; Invs^inv/Invs⁺ (more

)

Structure	Level	Pattern	Image	Note
TS12: notochord	Present		2A
TS12: notochordal plate	Present	Regionally restricted	2A	Expressed around the posterior notochord and in the notochord itself in a horse-shoe pattern.

2A' ©

Specimen 2A': embryonic day 8.0; Invs^inv/Invs⁺ (more

)

Structure	Level	Pattern	Image	Note
TS12: notochord	Present	Uniform	2A'

2A" ©

Specimen 2A": embryonic day 8.0; Invs^inv/Invs⁺ (more

)

Structure	Level	Pattern	Image	Note
TS12: notochordal plate	Present	Regionally restricted	2A"	Expressed in the posterior notochord. Much stronger signals in the crown cells.

2B left ©

Specimen 2B left: embryonic day 8.5; Invs^inv/Invs^inv (more

)

Structure	Level	Pattern	Image	Note
TS13: notochordal plate	Present	Regionally restricted	2B left	Expressed in the posterior notochord in two distinct stripes.
TS13: heart	Present	Regionally restricted	2B left	Expression was inverted, in the right heart tube.

2B right ©

Specimen 2B right: embryonic day 8.5; Invs^inv/Invs^inv (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	2B right	Expression was inverted, in the right heart tube.

2B' ©

Specimen 2B': embryonic day 8.5; Invs^inv/Invs^inv (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	2B'	Expression was inverted, in the right heart tube.

2C left ©

Specimen 2C left: embryonic day 8.5; Dnah11^iv/Dnah11^iv (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	2C left	Expressed in the left linear heart (seen in 23% embryos).
TS13: notochordal plate	Strong	Regionally restricted	2C left	Expressed around the posterior notochord and in the notochord itself.
TS13: notochord	Strong		2C left

2C right ©

Specimen 2C right: embryonic day 8.5; Dnah11^iv/Dnah11^iv (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	2C right	Expressed in the left linear heart.

2D left ©

Specimen 2D left: embryonic day 8.5; Dnah11^iv/Dnah11^iv (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	2D left	Expressed on the right (seen in 20% embryos).
TS13: notochordal plate	Strong	Regionally restricted	2D left	Expressed around the posterior notochord and in the notochord itself as two distinct stripes.
TS13: notochord	Strong		2D left

2D right ©

Specimen 2D right: embryonic day 8.5; Dnah11^iv/Dnah11^iv (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	2D right	Expressed on the right.

2E ©

Specimen 2E: embryonic day 8.5; Dnah11^iv/Dnah11^iv (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	2E	Expressed in both sides (seen in 30% embryos).
TS13: notochord	Strong		2E

2F ©

Specimen 2F: embryonic day 8.5; Dnah11^iv/Dnah11^iv (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Strong		2F
TS13: heart	Absent		2F	Seen in 27% embryos.

3A ©

Specimen 3A: embryonic day 8.5; Cfc1^tm1Cbm/Cfc1^tm1Cbm (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	3A	Expressed in the left linear heart.

3A' ©

Specimen 3A': embryonic day 8.5; Cfc1^tm1Cbm/Cfc1^tm1Cbm (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted	3A'	Expressed in the left linear heart.

Specimen not shown: embryonic day 9.0-9.5 (more

)

Structure	Level	Pattern	Image	Note
TS14: atrioventricular canal	Present	Regionally restricted		As heart looping proceeded, expression was progressingly restricted to the atrioventricular boundary. Subsequently spread bilaterally toward the ventral part.

Specimen not shown: embryonic day 8.5; Invs^inv/Invs⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted		Expressed in the left linear heart.

Specimen not shown: embryonic day 8.5; Cfc1^tm1Cbm/Cfc1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: heart	Present	Regionally restricted		Expressed in the left linear heart.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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