Tg(Amigo2-cre)1Sieg
Transgene Detail
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| Symbol: |
Tg(Amigo2-cre)1Sieg |
| Name: |
transgene insertion 1, Steven A Siegelbaum |
| MGI ID: |
MGI:5565335 |
| Synonyms: |
Amigo2-Cre |
| Transgene: |
Tg(Amigo2-cre)1Sieg Location: unknown
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| Alliance: |
Tg(Amigo2-cre)1Sieg page
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| Transgene Type: |
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Transgenic (Recombinase) |
| Mutation: |
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Insertion
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Tg(Amigo2-cre)1Sieg expression driven by
1 gene
Transgene expression driven by:
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Mutation details: The Amigo2 gene contained in a bacterial artificial chromosome (BAC; RP23-288P18) was modified with recombineering techniques so that a cre-HSV-polyA cassette(Cre recombinase sequence with an intron in the middle of the gene to enhance mammalian expression and followed by an HSV polyadenylation signal and a portion of a neo cassette) was inserted at the translation start site. This construct was injected into B6CBAF2 pronuclei and 6 founders were obtained. When crossed to Ai14 reporter mice, CA2-specific recombinase activity was not detected in offspring of any of the crosses. With injection of an adenovirus-EYFP reporter construct into the hippocampi of adult cre animals from the 6 founder lines, one line exhibited CA2-specific reporter expression and this line was used for all subsequent experiments.
(J:208892)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Amigo2
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:208892 Hitti FL, et al., The hippocampal CA2 region is essential for social memory. Nature. 2014 Apr 3;508(7494):88-92 |
| All: |
13 reference(s) |
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Analysis Tools
