Antibody:	Anti-Perlecan clone A7L6
Detection system:	Secondary antibody coupled to rhodamine

Structure	Level	Pattern	Image	Note
TS21: blood vessel	Present	Regionally restricted	2B E13	Expression was detected in the basement membranes of blood vessels.
TS21: nasal cavity lamina propria	Present	Regionally restricted	2B E13	Expression was detected underneath the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS23: blood vessel	Present	Regionally restricted	2B E15	Expression was detected in the basement membranes of blood vessels.

Structure	Level	Pattern	Image	Note
TS25: blood vessel	Present	Regionally restricted	2B E17	Expression was detected in the basement membranes of blood vessels.
TS25: main olfactory bulb	Present	Regionally restricted	2B E17	Expression was detected in the olfactory nerve layer in the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS27: blood vessel	Present	Regionally restricted	2B PND0	Expression was detected in the basement membranes of blood vessels.

Structure	Level	Pattern	Image	Note
TS27: blood vessel	Present	Regionally restricted	2B PND2	Expression was detected in the basement membranes of blood vessels.

Structure	Level	Pattern	Image	Note
TS28: blood vessel	Present	Regionally restricted	2B PND4	Expression was detected in the basement membranes of blood vessels.

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present	Regionally restricted	3C	Expression was detected in the basement membrane of the olfactory epithelium.
TS23: nasal septum lamina propria	Present	Regionally restricted	3C	Expression was detected in the lamina propria surrounding the fascicles of olfactory nerve axons labeled with Ncam.

Structure	Level	Pattern	Image	Note
TS27: olfactory epithelium	Present	Regionally restricted	3D	Expression was detected in the basement membrane of the olfactory epithelium.
TS27: nasal septum lamina propria	Present	Regionally restricted	3D	Expression was detected in the lamina propria surrounding the fascicles of olfactory nerve axons labeled with Ncam.

Structure	Level	Pattern	Image	Note
TS23: olfactory nerve	Present	Regionally restricted	4C	Expression was detected in the olfactory ensheathing cells surrounding the olfactory sensory neuron axons crossing the cribriform plate. Expression was not seen surrounding every bundle at E15.5.

Structure	Level	Pattern	Image	Note
TS27: olfactory nerve	Present	Regionally restricted	4D	Expression was detected in the olfactory ensheathing cells surrounding the olfactory sensory neuron axons crossing the cribriform plate. At P2, expression appeared to surround all axon bundles.

Structure	Level	Pattern	Image	Note
TS23: olfactory nerve	Present	Regionally restricted	5C	At E15.5, expression was found surrounding the entry zone, where the olfactory nerve enters the olfactory bulb. However, there was no expression in olfactory ensheathing cells within the entry zone.
TS23: blood vessel	Present	Regionally restricted	5C	Expression was detected in the basement membrane of blood vessels in the entry zone of the olfactory nerve fascicles as they enter the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS27: olfactory nerve	Present	Regionally restricted	5D	At P2, expression was detected surrounding olfactory nerve fascicles within the entry zone, where the olfactory nerve enters the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb	Absent		6C	Expression was not detected in the olfactory nerve layer in the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	6D	Expression was seen in the inner olfactory nerve layer surrounding fascicles of axons.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb	Absent		7A	There was no expression in the olfactory nerve layer at E15.5.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb olfactory nerve layer	Present	Regionally restricted	7B	Co-expression with laminin-1 was noted in the olfactory ensheathing cells.