Gene Expression Data
Assay Details
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Assay
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Reference:
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J:137171
Shay EL, et al., Dynamic expression patterns of ECM molecules in the developing mouse olfactory pathway. Dev Dyn. 2008 Jul;237(7):1837-50
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5563418
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Gene symbol:
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Tnc
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Gene name:
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tenascin C
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Results
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Specimen
2D E13:
embryonic day 13.5
(more  )
Note: Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
Specimen
2D E13:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: olfactory lobe |
Present |
Regionally restricted |
2D E13
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Expression was detected in the olfactory bulb in layers deep to the olfactory nerve layer.
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Specimen
2D E15:
embryonic day 15.5
(more )
Note: Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
Specimen
2D E15:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: main olfactory bulb |
Present |
Regionally restricted |
2D E15
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Expression was detected in the olfactory bulb in layers deep to the olfactory nerve layer.
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Specimen
2D E17:
embryonic day 17.5
(more )
Note: Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
Specimen
2D E17:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: main olfactory bulb |
Present |
Regionally restricted |
2D E17
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Expression was detected in the olfactory bulb in layers deep to the olfactory nerve layer.
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Specimen
2D PND0:
postnatal day 0
(more )
Note: Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
Specimen
2D PND0:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: main olfactory bulb |
Present |
Regionally restricted |
2D PND0
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Expression was detected in the olfactory bulb in layers deep to the olfactory nerve layer.
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Specimen
2D PND2:
postnatal day 2
(more )
Note: Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
Specimen
2D PND2:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: main olfactory bulb |
Present |
Regionally restricted |
2D PND2
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Expression was detected in the olfactory bulb in layers deep to the olfactory nerve layer.
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Specimen
2D PND4:
postnatal day 4
(more )
Note: Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
Specimen
2D PND4:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: main olfactory bulb |
Present |
Regionally restricted |
2D PND4
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Expression was detected in the olfactory bulb in layers deep to the olfactory nerve layer.
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Specimen
3C:
embryonic day 15.5
(more )
Note: Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
Specimen
3C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
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Specimen
3D:
postnatal day 2
(more )
Note: Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
Specimen
3D:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: nasal septum lamina propria |
Weak |
Single cells |
3D
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Expression was detected at low levels in some olfactory ensheathing cells surrounding olfactory nerve axon bundles, colocalizing with perlecan.
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| TS27: nasal septum |
Present |
Regionally restricted |
3D
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Expression was detected in cells of connective tissue lining the nasal septum.
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Specimen
4C:
embryonic day 15.5
(more )
Note: Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
Specimen
4C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
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Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: olfactory nerve |
Weak |
Regionally restricted |
4C
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Expression was detected at low levels in the olfactory ensheathing cells surrounding the olfactory nerve axons as it passes through the cribriform plate. There was some overlap with perlecan.
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Specimen
4D:
postnatal day 2
(more )
Note: Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
Specimen
4D:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
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Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: olfactory nerve |
Present |
Regionally restricted |
4D
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At P2, expression was detected at much higher levels in the olfactory ensheathing cells surround the olfactory nerve axons as it passes through the cribriform plate.
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| TS27: ethmoid bone cribriform plate |
Present |
Regionally restricted |
4D
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Expression was detected in cells of the connective tissue lining the cribriform plate.
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Specimen
5C:
embryonic day 15.5
(more )
Note: Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
Specimen
5C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
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Cryosection
|
|
Note:
|
Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: main olfactory bulb |
Present |
Regionally restricted |
5C
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Expression was detected in the olfactory bulb, but not in the entry zone of the olfactory nerve into the olfactory nerve layer.
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Specimen
5D:
postnatal day 2
(more )
Note: Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
Specimen
5D:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: main olfactory bulb |
Present |
Regionally restricted |
5D
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Expression was detected in the olfactory bulb, but not in the entry zone of the olfactory nerve into the olfactory nerve layer.
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Specimen
6C:
embryonic day 15.5
(more )
Note: Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
Specimen
6C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: main olfactory bulb |
Present |
Regionally restricted |
6C
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Expression was detected in the dentric zone.
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Specimen
6D:
postnatal day 2
(more )
Note: Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
Specimen
6D:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - Hspg2 (assay MGI:5563371).
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Specimen
7C:
embryonic day 15.5
(more )
Note: Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - CSPGs.
Specimen
7C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - CSPGs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: main olfactory bulb |
Present |
Regionally restricted |
7C
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Expression was detected in the dentric zone.
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Specimen
7D:
postnatal day 2
(more )
Note: Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - CSPGs.
Specimen
7D:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Tnc; blue - Ncam1 (assay MGI:5563046); red - CSPGs.
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