Gene Expression Data
Assay Details
Assay
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Reference:
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J:143210
Zhou CJ, et al., Ocular coloboma and dorsoventral neuroretinal patterning defects in Lrp6 mutant eyes. Dev Dyn. 2008 Dec;237(12):3681-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5554718
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Gene symbol:
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Gfap
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Gene name:
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glial fibrillary acidic protein
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Antibody:
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anti-GFAP
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2G:
embryonic day 18.5
(more )
Specimen
2G:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: lens |
Present |
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2G
|
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TS26: eye |
Present |
Regionally restricted |
2G
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Expression is in the mesenchyme.
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Specimen
2H:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Lrp6Gt(Ex187)Byg/Lrp6Gt(Ex187)Byg
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lens |
Present |
|
2H
|
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TS26: eye |
Weak |
Regionally restricted |
2H
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A group of mesenchymal cells is missing in the mutant eyelid.
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