Probe:	Sip1 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Data was shown in Table 1.

Structure	Level	Pattern	Image	Note
TS11: head mesenchyme	Present
TS11: neural ectoderm	Present
TS11: primitive streak	Present
TS11: embryo endoderm	Absent
TS11: node	Absent
TS11: notochordal plate	Absent			prechordal plate
TS11: embryo ectoderm	Present	Regionally restricted		Expressed in primitive ectoderm.
TS11: embryo mesoderm	Present	Regionally restricted		Expressed in paraxial mesoderm.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present
TS13: future prosencephalon	Present
TS13: future spinal cord	Present
TS13: head mesenchyme	Present
TS13: lateral plate mesoderm	Present
TS13: neural crest	Present
TS13: paraxial mesenchyme	Present
TS13: rhombomere	Present
TS13: gut endoderm	Absent
TS13: node	Absent
TS13: notochord	Absent

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present
TS15: future forebrain	Present
TS15: future hindbrain	Present
TS15: future midbrain	Present
TS15: future spinal cord	Present
TS15: trigeminal preganglion	Present
TS15: tail unsegmented mesenchyme	Present			presomitic mesoderm
TS15: foregut epithelium	Absent
TS15: hindgut epithelium	Absent
TS15: limb mesenchyme	Absent
TS15: midgut epithelium	Absent
TS15: myotome	Absent
TS15: notochord	Absent

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present
TS17: diencephalon	Present
TS17: dorsal root ganglion	Present
TS17: facial ganglion	Present
TS17: future spinal cord	Present
TS17: glossopharyngeal ganglion	Present
TS17: hindbrain	Present
TS17: lung mesenchyme	Present
TS17: midbrain	Present
TS17: telencephalon	Present
TS17: trigeminal ganglion	Present
TS17: vagus ganglion	Present
TS17: tail unsegmented mesenchyme	Present			presomitic mesoderm
TS17: hindgut epithelium	Absent
TS17: limb mesenchyme	Absent
TS17: midgut epithelium	Absent
TS17: myotome	Absent
TS17: notochord	Absent
TS17: trachea epithelium	Absent
TS17: gut epithelium	Absent			No expression in foregut, midgut and hindgut endodermal tissues.

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present
TS19: diencephalon	Present
TS19: dorsal root ganglion	Present
TS19: facial ganglion	Present
TS19: future spinal cord	Present
TS19: glossopharyngeal ganglion	Present
TS19: hindbrain	Present
TS19: lens vesicle	Present
TS19: midbrain	Present
TS19: trigeminal ganglion	Present
TS19: vagus ganglion	Present
TS19: telencephalon	Present	Regionally restricted		Expressed in telencephalon, but no expression in choroid invagination.
TS19: Meckel's cartilage pre-cartilage condensation	Absent
TS19: Rathke's pouch	Absent
TS19: esophagus epithelium	Absent
TS19: main bronchus epithelium	Absent
TS19: mesonephros	Absent
TS19: myotome	Absent
TS19: notochord	Absent
TS19: pancreas primordium epithelium	Absent
TS19: stomach epithelium	Absent
TS19: trachea epithelium	Absent
TS19: urogenital ridge	Absent
TS19: maxillary process mesenchyme	Present	Regionally restricted		Expressed in the lateral part, not the medial part.
TS19: metanephric mesenchyme	Absent			metanephric blastema
TS19: limb mesenchyme	Present	Regionally restricted		Expressed in the precartilageous condensation.
TS19: liver	Present	Regionally restricted		Expressed in the endodermal tissue.
TS19: tail unsegmented mesenchyme	Present			presomitic mesoderm
TS19: surface ectoderm	Present	Regionally restricted		Expressed in facial surface ectoderm.
TS19: autonomic nervous system	Present	Regionally restricted		Expressed in accessory ganglion.