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Gene Expression Data
Assay Details
Assay
Reference: J:108556 Buttler K, et al., Mesenchymal cells with leukocyte and lymphendothelial characteristics in murine embryos. Dev Dyn. 2006 Jun;235(6):1554-1562
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5551773
Gene symbol: Lyve1
Gene name: lymphatic vessel endothelial hyaluronan receptor 1
Antibody: Anti-Lyve1
Assay notes: Two antibodies were described in the reference. This assay could have used either MGI:5551738 or MGI:5551746.
Results
Specimen 5A,B: embryonic day 10.5 (more )
Note: Double labeled: red - Lyve1; green - Ptprc (CD45; assay MGI:5551777).
Structure Level Pattern Image Note
TS17: neural tube Absent 5A
TS17: mediastinum Present Regionally restricted 5B , 5A The mediastinal region contained cells co-expressing Lyve1 and CD45/Ptprc and single immunopositive cells of both types.
Specimen 5C: embryonic day 11.5 (more )
Note: Double labeled: red - Lyve1; green - Ptprc (CD45; assay MGI:5551777).
Structure Level Pattern Image Note
TS19: dermatome Present Regionally restricted 5C Lyve1 and CD45 double positive cells were detected in dermatomes laterally from the neural tube.
TS19: mediastinum Present Regionally restricted 5C The number of cells co-expressing Lyve1/CD45 in the mediastinum was greater at E11.5.
TS19: neural tube Absent 5C
Specimen 6A,B: embryonic day 12.5 (more )
Note: Double labeled: red - Lyve1; green - Ptprc (CD45; assay MGI:5551777).
Structure Level Pattern Image Note
TS20: dermis Present Regionally restricted 6B , 6A Lyve1 and CD45/Ptprc double immunopositive cells were detected.
TS20: mediastinum Present Regionally restricted 6B , 6A Lyve1 and CD45/Ptprc double immunopositive cells were detected.
Specimen 6C,D: embryonic day 13.5 (more )
Note: Double labeled: red - Lyve1; green - Ptprc (CD45; assay MGI:5551777).
Structure Level Pattern Image Note
TS21: lymphatic system Present Regionally restricted 6D , 6C Lyve1/CD45 double immunopositive cells with a flat morphology were detected in the lining of lymphatic vessels.
Specimen 8: embryonic day 11.5 (more )
Note: Double labeled: red - Lyve1; green - Pecam1 (assay MGI:5551826).
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted 8 Co-expression with CD31/Pecam1 was detected in the jugular lymph sac.
TS19: dermatome Present Scattered 8
Specimen 9A,B,C: embryonic day 12.5 (more )
Note: Double labeled: red - Lyve1; green - Pecam1 (assay MGI:5551826).
Structure Level Pattern Image Note
TS20: dermis Present Scattered 9B , 9A Some co-expression with CD31/Pecam1 was observed in the scattered Lyve1 immunopositive cells. The closer to the jugular lymph sac, the stronger the CD31 co-expression.
TS20: mediastinum Present Scattered 9A , 9C Some co-expression with CD31/Pecam1 was observed in the scattered Lyve1 immunopositive cells. The closer to the jugular lymph sac, the stronger the CD31 co-expression.
Specimen Table 1 E10.5 Lyve1/CD31: embryonic day 10.5 (more )
Note: Double labeled: red - Lyve1; green - Pecam1 (assay MGI:5551826).
Structure Level Pattern Image Note
TS17: cardinal vein Present
Specimen Table 1 E11.5 Lyve1/CD31: embryonic day 11.5 (more )
Note: Double labeled: red - Lyve1; green - Pecam1 (assay MGI:5551826).
Structure Level Pattern Image Note
TS19: cardinal vein Present
TS19: dermatome Present Single cells
TS19: mediastinum Present Single cells
TS19: lymphatic system Present Regionally restricted Expression was detected in lymph sacs.
Specimen Table 1 E12.5 Lyve1/CD31: embryonic day 12.5 (more )
Note: Double labeled: red - Lyve1; green - Pecam1 (assay MGI:5551826).
Structure Level Pattern Image Note
TS20: lymphatic system Present Regionally restricted Expression was detected in lymph sacs.
TS20: cardinal vein Present
TS20: dermis Present Single cells
TS20: mediastinum Present Single cells
Specimen Table 1 E13.5 Lyve1/CD31: embryonic day 13.5 (more )
Note: Double labeled: red - Lyve1; green - Pecam1 (assay MGI:5551826).
Structure Level Pattern Image Note
TS21: lymphatic system Present Regionally restricted Expression was detected in lymph sacs.
TS21: dermis Present Single cells
TS21: mediastinum Present Single cells
Specimen Table 1 E10.5 Lyve1/CD45: embryonic day 10.5 (more )
Note: Double labeled: red - Lyve1; green - Ptprc (CD45; assay MGI:5551777).
Structure Level Pattern Image Note
TS17: mediastinum Present Single cells
Specimen Table 1 E11.5 Lyve1/CD45: embryonic day 11.5 (more )
Note: Double labeled: red - Lyve1; green - Ptprc (CD45; assay MGI:5551777).
Structure Level Pattern Image Note
TS19: dermatome Present Single cells
TS19: mediastinum Present Single cells
Specimen Table 1 E12.5 Lyve1/CD45: embryonic day 12.5 (more )
Note: Double labeled: red - Lyve1; green - Ptprc (CD45; assay MGI:5551777).
Structure Level Pattern Image Note
TS20: dermis Present Single cells
TS20: mediastinum Present Single cells
Specimen Table 1 E13.5 Lyve1/CD45: embryonic day 13.5 (more )
Note: Double labeled: red - Lyve1; green - Ptprc (CD45; assay MGI:5551777).
Structure Level Pattern Image Note
TS21: dermis Present Single cells
TS21: mediastinum Present Single cells
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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