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Gene Expression Data
Assay Details
Assay
Reference: J:108556 Buttler K, et al., Mesenchymal cells with leukocyte and lymphendothelial characteristics in murine embryos. Dev Dyn. 2006 Jun;235(6):1554-1562
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5551777
Gene symbol: Ptprc
Gene name: protein tyrosine phosphatase receptor type C
Antibody: Anti-CD45
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Two antibodies were described in the reference. This assay could have used either MGI:5551740 or MGI:5551744.
Results
Specimen 5A,B: embryonic day 10.5 (more )
Note: Double labeled: green - Ptprc; red - Lyve1 (assay MGI:5551773).
Structure Level Pattern Image Note
TS17: mediastinum Present Regionally restricted 5A , 5B The mediastinal region contained cells co-expressing Lyve1 and CD45/Ptprc and single immunopositive cells of both types.
Specimen 5C: embryonic day 11.5 (more )
Note: Double labeled: green - Ptprc; red - Lyve1 (assay MGI:5551773).
Structure Level Pattern Image Note
TS19: dermatome Present Regionally restricted 5C Lyve1 and CD45 double positive cells were detected in dermatomes laterally from the neural tube.
TS19: mediastinum Present Regionally restricted 5C The number of cells co-expressing Lyve1/CD45 in the mediastinum was greater at E11.5.
TS19: neural tube Present Regionally restricted 5C Numerous expressing cells were detected.
Specimen 6A,B: embryonic day 12.5 (more )
Note: Double labeled: green - Ptprc; red - Lyve1 (assay MGI:5551773).
Structure Level Pattern Image Note
TS20: dermis Present Regionally restricted 6B , 6A Lyve1 and CD45/Ptprc double immunopositive cells were detected.
TS20: mediastinum Present Regionally restricted 6B , 6A Lyve1 and CD45/Ptprc double immunopositive cells were detected.
Specimen 6C,D: embryonic day 13.5 (more )
Note: Double labeled: green - Ptprc; red - Lyve1 (assay MGI:5551773).
Structure Level Pattern Image Note
TS21: lymphatic system Present Regionally restricted 6C , 6D Lyve1/CD45 double immunopositive cells with a flat morphology were detected in the lining of lymphatic vessels.
Specimen 7A: embryonic day 11.5 (more )
Note: Double labeled: green - Ptprc; red - Prox1 (assay MGI:5551774).
Structure Level Pattern Image Note
TS19: dermatome Present Scattered 7A Prox1/CD45 double positive cells were scattered in the dermatome.
Specimen 7B,C: embryonic day 13.5 (more )
Note: Double labeled: green - Ptprc; red - Prox1 (assay MGI:5551774).
Structure Level Pattern Image Note
TS21: lymphatic system Present Single cells 7B , 7C Weak expression was noted in a cell in a lymphatic vessel cell that co-expressed Prox1.
Specimen Table 1 E10.5 Lyve1/CD45: embryonic day 10.5 (more )
Note: Double labeled: green - Ptprc; red - Lyve1 (assay MGI:5551773).
Structure Level Pattern Image Note
TS17: mediastinum Present Single cells
Specimen Table 1 E11.5 Lyve1/CD45: embryonic day 11.5 (more )
Note: Double labeled: green - Ptprc; red - Lyve1 (assay MGI:5551773).
Structure Level Pattern Image Note
TS19: dermatome Present Single cells
TS19: mediastinum Present Single cells
Specimen Table 1 E12.5 Lyve1/CD45: embryonic day 12.5 (more )
Note: Double labeled: green - Ptprc; red - Lyve1 (assay MGI:5551773).
Structure Level Pattern Image Note
TS20: dermis Present Single cells
TS20: mediastinum Present Single cells
Specimen Table 1 E13.5 Lyve1/CD45: embryonic day 13.5 (more )
Note: Double labeled: green - Ptprc; red - Lyve1 (assay MGI:5551773).
Structure Level Pattern Image Note
TS21: dermis Present Single cells
TS21: mediastinum Present Single cells
Specimen Table 1 E11.5 Prox1/CD45: embryonic day 11.5 (more )
Note: Double labeled: green - Ptprc; red - Prox1 (assay MGI:5551774).
Structure Level Pattern Image Note
TS19: dermatome Present Single cells
TS19: mediastinum Present Single cells
Specimen Table 1 E12.5 Prox1/CD45: embryonic day 12.5 (more )
Note: Double labeled: green - Ptprc; red - Prox1 (assay MGI:5551774).
Structure Level Pattern Image Note
TS20: dermis Present Single cells
TS20: mediastinum Present Single cells
Specimen Table 1 E13.5 Prox1/CD45: embryonic day 13.5 (more )
Note: Double labeled: green - Ptprc; red - Prox1 (assay MGI:5551774).
Structure Level Pattern Image Note
TS21: dermis Present Single cells
TS21: mediastinum Present Single cells
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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