Probe:	Sip1 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		2Aa

Structure	Level	Pattern	Image	Note
TS11: primitive endoderm	Present	Regionally restricted	2Ac	Expressed more strongly in the primitive endoderm in the posterior part of the embryo.

Structure	Level	Pattern	Image	Note
TS12: embryo ectoderm	Present	Widespread	2Ba
TS12: embryo endoderm	Present	Widespread	2Ba
TS12: embryo mesenchyme	Present	Widespread	2Ba
TS12: neural fold	Strong	Regionally restricted	2Ba	Strongest expression in the anterior neural fold.
TS12: paraxial mesenchyme	Present		2Ba

Structure	Level	Pattern	Image	Note
TS12: node	Absent		2Bc
TS12: notochordal plate	Absent		2Bc	Absent in the prechordal plate anteriorly extending from the node.
TS12: neural fold	Strong	Regionally restricted	2Bc	Strongest expression in the anterior neural fold.
TS12: paraxial mesenchyme	Present		2Bc

Structure	Level	Pattern	Image	Note
TS12: future spinal cord neural plate	Present		2Be

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		2Bg

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present	Regionally restricted	2Ca left	Expressed throughout the neural plate/tube, with markedly decreased expression in the anterior margin and rhombomeres 3 and 5.
TS13: anterior pro-rhombomere neural fold	Weak	Regionally restricted	2Ca left	Low expression in the anterior margin.
TS13: rhombomere 3	Weak		2Ca left
TS13: rhombomere 5	Weak		2Ca left

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present	Regionally restricted	2Ca right	Expressed throughout the neural plate/tube, with markedly decreased expression in the anterior margin and rhombomeres 3 and 5.
TS13: rhombomere 3	Weak		2Ca right
TS13: rhombomere 5	Weak		2Ca right

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present	Regionally restricted	2Ca inset	Expressed throughout the neural plate/tube, with markedly decreased expression in the anterior margin and rhombomeres 3 and 5.
TS13: rhombomere 3	Weak		2Ca inset
TS13: rhombomere 5	Weak		2Ca inset

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present		2Cc
TS13: future prosencephalon	Present		2Cc

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Absent		2Ce
TS13: node	Absent		2Ce
TS13: notochord	Absent		2Ce
TS13: neural tube	Present		2Ce
TS13: somite	Present		2Ce

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present		2Cg
TS13: node	Absent		2Cg

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		3Aa
TS15: neural tube	Present		3Aa
TS15: trigeminal preganglion	Present		3Aa

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		3Ba
TS17: trigeminal ganglion	Present		3Ba

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		3Bc

Structure	Level	Pattern	Image	Note
TS19: embryo	Present		3Ca

Structure	Level	Pattern	Image	Note
TS19: maxilla primordium	Strong	Regionally restricted	3Cc	Expressed in the lateral part.
TS19: nasal pit	Absent		3Cc

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	3Ce	Expressed in the corpus striatum.
TS19: trigeminal ganglion	Present		3Ce
TS19: neural tube ventricular layer	Present	Regionally restricted	3Ce	Expressed in the ventricular zone where neuroepithelial stem cells reside.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		3Cg
TS19: neural tube ventricular layer	Strong	Regionally restricted	3Cg	Expressed in the ventricular zone where neuroepithelial stem cells reside.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present			Same as in the wild-type.