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Afmidtm1b(EUCOMM)Wtsi
Targeted Allele Detail
Summary
Symbol: Afmidtm1b(EUCOMM)Wtsi
Name: arylformamidase; targeted mutation 1b, Wellcome Trust Sanger Institute
MGI ID: MGI:5548841
Gene: Afmid  Location: Chr11:117716750-117730734 bp, + strand  Genetic Position: Chr11, 82.96 cM, cytoband E2
Alliance: Afmidtm1b(EUCOMM)Wtsi page
IMPC: Afmid gene page
Mutation
origin
Mutant Cell Line:  EPD0155_6_A07
Germline Transmission:  Earliest citation of germline transmission: J:204739
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsCre-mediated excision of the parental Afmidtm1a(EUCOMM)Wtsi allele resulted in the removal of the promoter-driven neomycin selection cassette and the critical exon 2 leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. qRT-PCR on liver and kidney confirmed absence of RNA in homozygotes. RNA expression of the immediately adjacent Tk1 gene is reduced by 52% in the kidney and 94% in liver of homozygotes. (J:204739, J:226537)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 11 assay results
In Structures Affected by this Mutation: 4 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Afmid Mutation:  37 strains or lines available
References
Original:  J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023;
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory