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Gene Expression Data
Assay Details
Assay
Reference: J:97081 Lees-Murdock DJ, et al., DNA methyltransferase expression in the mouse germ line during periods of de novo methylation. Dev Dyn. 2005 Apr;232(4):992-1002
Assay type: RT-PCR
MGI Accession ID: MGI:5546011
Gene symbol: Dnmt3b
Gene name: DNA methyltransferase 3B
Probe: Dnmt3b-F7, Dnmt3b-R7
Assay notes: This blot contains the results of a multiplex reaction; other bands are for beta-actin as control.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex Note
- Mol. Wt. Marker Lane
testis e12.5 E12.5 TS20: germ cell of testis
TS20: testis 		Present Present Present 1 µg; total RNA HsdOla:TO Male Germ cells were purified without culture (purity 85%).
testis e15.5 E15.5 TS23: germ cell of testis
TS23: testis 		Present Present Present 1 µg; total RNA HsdOla:TO Male Germ cells were purified without culture (purity 85%).
testis e17.5 E17.5 TS25: germ cell of testis
TS25: testis 		Present Present Present 1 µg; total RNA HsdOla:TO Male Germ cells were purified without culture (purity 85%).
testis 12bpp P12 TS28: testis Present Present Present 1 µg; total RNA HsdOla:TO Male
testis 24dpp P24 TS28: testis Present Present Present 1 µg; total RNA HsdOla:TO Male
testis adult P adult TS28: testis Present Present Present 1 µg; total RNA HsdOla:TO Male
e8.5 embryo E8.5 TS13: embryo Present Present Present 1 µg; total RNA HsdOla:TO Not Specified
lung P adult TS28: lung Present Present Present 1 µg; total RNA HsdOla:TO Not Specified
spleen P adult TS28: spleen Present Present Present 1 µg; total RNA HsdOla:TO Not Specified
liver P adult TS28: liver Present Present Present 1 µg; total RNA HsdOla:TO Not Specified
- Mol. Wt. Marker Lane
Notes:
(a) +21 +22 band
(b) -21 +22 band
(c) -21 -22 band
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory