Probe:	Thg-1pit cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	A second probe MGI:5544819 gave comparable results.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	1A	Expression was detected at the level of all ventricles.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Strong		1C	Strong signal was observed at the fourth ventricle, with particular reference to the rhombic lip.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex subventricular zone	Present		1D
TS20: telencephalon mantle layer	Present		1D
TS20: lateral ganglionic eminence	Present		1D

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present	Widespread	2B , 2A	Expression was detected in the entire external granule layer, including both the most superficial layer (rapidly proliferating) and the deeper one (postmitotic, differentiating).

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present	Regionally restricted	2C	Expression was detected in the subventricular zone of the anterior lateral ventricles.
TS28: rostral migratory stream	Strong	Regionally restricted	2C	Strong expression was detected in the entire rostral migratory stream. Expression was also detected in the ventral migratory mass.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Strong		2D , 2E	Expression was detected in differentiated granule cells.
TS28: main olfactory bulb	Strong		2E , 2D	Expression was detected in differentiated granule cells and in periglomerular cells.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	2F	Expression was detected in basal forebrain interneurons.
TS28: islands of Calleja	Present	Regionally restricted	2F	Expression was detected in island of Calleja interneurons.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		2G

Structure	Level	Pattern	Image	Note
TS28: medulla oblongata	Present	Scattered
TS28: pons	Present	Scattered

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Strong		4B , 4A

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4C

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Absent		4D

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Trace	Single cells

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Present		5B , 5A	Expression was detected in granule cells.
TS28: main olfactory bulb	Present	Regionally restricted	5A , 5B	Expression was detected in granule cells and periglomerular cells.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Present		5C

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Present		5D

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	5E	Expression was detected in the entire rostral migratory stream.
TS28: telencephalon subventricular zone	Present	Regionally restricted	5E	Expression in subventricular zone of anterior lateral ventricle.
TS28: main olfactory bulb	Present		5E

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	5F	Expression was detected in cells derived from the ventral migratory mass in the basal forebrain.
TS28: islands of Calleja	Present		5F
TS28: olfactory tubercle	Present		5F

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		5G

Structure	Level	Pattern	Image	Note
TS28: medulla oblongata	Present	Scattered
TS28: pons	Present	Scattered