Probe:	Plag1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Authors described two probes that gave identical results. Either MGI:5543378 or MGI:2026252 could have been used in this assay.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1C	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS15: future midbrain	Present	Regionally restricted	1C	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS15: telencephalon	Present	Regionally restricted	1C	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS15: future hindbrain	Weak		1C
TS15: future spinal cord	Weak	Regionally restricted	1C	Lower levels were detected in the caudal spinal cord.
TS15: Rathke's pouch	Present		1C
TS15: branchial arch	Present		1C
TS15: forelimb bud	Present		1C
TS15: somite	Present	Regionally restricted	1C	Expression was detected in caudal somites.
TS15: embryo	Present	Regionally restricted	1C	Expression was detected in intersomital regions.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1F	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS15: future midbrain	Present	Regionally restricted	1F	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS15: telencephalon	Present	Regionally restricted	1F	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS15: future hindbrain	Weak		1F
TS15: olfactory placode	Weak		1F

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Weak	Regionally restricted	1I	Low expression was detected in the caudal spinal cord.
TS15: somite	Present	Regionally restricted	1I	Expression was detected in caudal somites.
TS15: forelimb bud	Present		1I
TS15: embryo	Present	Regionally restricted	1I	Expression was detected in intersomital regions.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2C	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS17: midbrain	Present	Regionally restricted	2C	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS17: telencephalon	Present	Regionally restricted	2C	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS17: hindbrain	Weak		2C
TS17: future spinal cord	Weak	Regionally restricted	2C	Lower levels were detected in the caudal spinal cord.
TS17: branchial arch	Present		2C
TS17: forelimb bud	Present		2C
TS17: hindlimb bud	Present		2C
TS17: somite	Present	Regionally restricted	2C	Expression was detected in caudal somites.
TS17: embryo	Present	Regionally restricted	2C	Expression was detected in intersomital regions.
TS17: optic cup	Present		2C
TS17: nasal pit	Weak		2C

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2F	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS17: midbrain	Present	Regionally restricted	2F	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS17: telencephalon	Present	Regionally restricted	2F	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS17: hindbrain	Weak		2F
TS17: Rathke's pouch	Present		2F
TS17: branchial arch	Present		2F
TS17: forelimb bud	Present		2F
TS17: hindlimb bud	Present		2F
TS17: nasal pit	Weak		2F

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2I	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS17: midbrain	Present	Regionally restricted	2I	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS17: telencephalon	Present	Regionally restricted	2I	Expression was highest in the dorsal telencephalon, diencephalon and midbrain.
TS17: hindbrain	Weak		2I
TS17: nasal pit	Weak		2I

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		2L
TS17: hindlimb bud	Present		2L
TS17: somite	Present	Regionally restricted	2L	Expression was detected in caudal somites.
TS17: embryo	Present	Regionally restricted	2L	Expression was detected in intersomital regions.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		2O

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	3C	Expression was highest in dorsal cortical progenitors.
TS20: cortical hem	Present		3C	Expression was detected in the cortical hem.
TS20: lateral ganglionic eminence	Weak		3C	Reduced expression was noted in the ventricular zone.
TS20: medial ganglionic eminence	Weak		3C	Reduced expression was noted in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present	Uniform	3L	Expression was uniform in the ventricular zone of the dorsal and ventral telencephalon.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Weak	Regionally restricted	3O	Expression was uniform in the ventricular zone of the dorsal and ventral telencephalon. Expression level was much reduced at P0.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Strong		4C , 4C'
TS27: cerebellum internal granule cell layer	Weak		4C , 4C'

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong		4G' , 4G
TS28: cerebellum internal granule cell layer	Weak		4G' , 4G

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		4K

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Weak	Regionally restricted	5C	Expression was detected in spinal cord progenitors, extending along the dorso-ventral axis.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Weak	Regionally restricted	5H	Expression was detected in spinal cord progenitors, extending along the dorso-ventral axis.
TS20: dorsal root ganglion	Absent		5H
TS20: sympathetic ganglion	Absent		5H

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Present	Regionally restricted	6C	Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Much lower levels were noted in postmitotic precursors and differentiated cells in the inner neuroblast layer.
TS20: lens vesicle	Present		6C

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present	Regionally restricted	6F	Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Much lower levels were noted in postmitotic precursors and differentiated cells in the inner neuroblast layer.
TS23: lens	Present		6F

Structure	Level	Pattern	Image	Note
TS27: retina outer nuclear layer	Present	Regionally restricted	6I	Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Much lower levels were noted in postmitotic precursors.
TS27: retina inner nuclear layer	Weak	Regionally restricted	6I	Expression was low in differentiated cells in the inner neuroblast layer.
TS27: lens	Absent		6I

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Strong	Regionally restricted	6L	Expression was high in differentiating photoreceptors in the outer nuclear layer.
TS28: retina inner nuclear layer	Present	Regionally restricted	6L	Expression was detected in a subset of cells in the inner nuclear layer.
TS28: retina ganglion cell layer	Present	Regionally restricted	6L	Expression was detected in a subset of cells in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Strong	Regionally restricted	6O	Expression was high in differentiating photoreceptors in the outer nuclear layer.
TS28: retina inner nuclear layer	Present	Regionally restricted	6O	Expression was detected in a subset of cells in the inner nuclear layer.
TS28: retina ganglion cell layer	Present	Regionally restricted	6O	Expression was detected in a subset of cells in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present	Widespread	7C	Expression was detected throughout the olfactory epithelium.
TS20: vomeronasal organ	Present		7C

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present	Regionally restricted	7H	Expression was detected throughout the olfactory epithelium with a concentration of transcripts in apical domains.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Absent		8C
TS20: gut epithelium	Absent		8C	Transcripts were not detected above background levels in the intestinal mucosa or myenteric plexus.
TS20: gut mesenchyme	Absent		8C	Transcripts were not detected above background levels in the intestinal mucosa or myenteric plexus.

Structure	Level	Pattern	Image	Note
TS20: gut epithelium	Absent		8H	Transcripts were not detected above background levels in the intestinal mucosa or myenteric plexus.
TS20: gut mesenchyme	Absent		8H	Transcripts were not detected above background levels in the intestinal mucosa or myenteric plexus.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Absent		8L

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Absent
TS17: facial ganglion	Absent
TS17: glossopharyngeal ganglion	Absent
TS17: trigeminal ganglion	Absent
TS17: vagus ganglion	Absent