Probe:	Plagl2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Authors described two probes that gave identical results. Either MGI:5543380 or MGI:2251726 could have been used in this assay.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Widespread	1B	Expression was detected both dorsally and ventrally.
TS15: future hindbrain	Present	Widespread	1B	Expression was detected both dorsally and ventrally.
TS15: future midbrain	Present	Widespread	1B	Expression was detected both dorsally and ventrally.
TS15: future spinal cord	Present	Widespread	1B	Expression was detected both dorsally and ventrally.
TS15: telencephalon	Present	Widespread	1B	Expression was detected both dorsally and ventrally.
TS15: branchial arch	Present		1B
TS15: forelimb bud	Present		1B
TS15: olfactory placode	Present		1B
TS15: optic vesicle	Present		1B
TS15: somite	Present	Regionally restricted	1B	Expression was detected in posterior somites.
TS15: hepatic primordium	Present		1B
TS15: lung epithelium	Present		1B
TS15: otocyst epithelium	Present		1B
TS15: gut epithelium	Present	Regionally restricted	1B	Expression was detected in the epithelial lining of the gut and stomach.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Widespread	1E	Expression was detected both dorsally and ventrally.
TS15: future hindbrain	Present	Widespread	1E	Expression was detected both dorsally and ventrally.
TS15: future midbrain	Present	Widespread	1E	Expression was detected both dorsally and ventrally.
TS15: telencephalon	Present	Widespread	1E	Expression was detected both dorsally and ventrally.
TS15: olfactory placode	Present		1E
TS15: optic vesicle	Present		1E

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		1H
TS15: somite	Present	Regionally restricted	1H	Expression was detected in posterior somites.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Widespread	2B	Expression was detected both dorsally and ventrally.
TS17: future spinal cord	Present	Widespread	2B	Expression was detected both dorsally and ventrally.
TS17: hindbrain	Present	Widespread	2B	Expression was detected both dorsally and ventrally.
TS17: midbrain	Present	Widespread	2B	Expression was detected both dorsally and ventrally.
TS17: telencephalon	Present	Widespread	2B	Expression was detected both dorsally and ventrally.
TS17: nasal pit	Present		2B
TS17: optic cup	Present	Regionally restricted	2B	Expression was detected in the optic vesicles.
TS17: branchial arch	Present		2B
TS17: forelimb bud	Present		2B
TS17: hindlimb bud	Present		2B
TS17: somite	Present	Regionally restricted	2B	Expression was detected in posterior somites.
TS17: gut epithelium	Present		2B
TS17: hepatic primordium	Present		2B
TS17: lung epithelium	Present		2B
TS17: otocyst epithelium	Present		2B
TS17: stomach epithelium	Present		2B

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Widespread	2E	Expression was detected both dorsally and ventrally.
TS17: hindbrain	Present	Widespread	2E	Expression was detected both dorsally and ventrally.
TS17: midbrain	Present	Widespread	2E	Expression was detected both dorsally and ventrally.
TS17: telencephalon	Present	Widespread	2E	Expression was detected both dorsally and ventrally.
TS17: Rathke's pouch	Present		2E
TS17: branchial arch	Present		2E
TS17: forelimb bud	Present		2E
TS17: hindlimb bud	Present		2E

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Widespread	2H	Expression was detected both dorsally and ventrally.
TS17: hindbrain	Present	Widespread	2H	Expression was detected both dorsally and ventrally.
TS17: midbrain	Present	Widespread	2H	Expression was detected both dorsally and ventrally.
TS17: telencephalon	Present	Widespread	2H	Expression was detected both dorsally and ventrally.
TS17: nasal pit	Present		2H
TS17: optic cup	Present	Regionally restricted	2H	Expression was detected in the optic vesicles.

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		2K
TS17: forelimb bud	Present		2K
TS17: hindlimb bud	Present		2K
TS17: somite	Present	Regionally restricted	2K	Expression was detected in posterior somites.

Structure	Level	Pattern	Image	Note
TS17: hepatic primordium	Present		2N
TS17: stomach epithelium	Present		2N

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present	Widespread	3B	Expression was detected throughout the dorsal and ventral ventricular zone.
TS20: cortical preplate	Absent		3B
TS20: cerebral cortex mantle layer	Ambiguous		3B	Expression was excluded from the ventral mantle zone.
TS20: cortical hem	Present		3B	Expression was detected in the cortical hem.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present	Uniform	3K	Expression was uniform in the ventricular zone of the dorsal and ventral telencephalon.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Weak	Regionally restricted	3N	Expression was uniform in the ventricular zone of the dorsal and ventral telencephalon. Expression level was much reduced at P0.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Strong		4B , 4B'
TS27: cerebellum internal granule cell layer	Weak		4B' , 4B

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong		4F' , 4F
TS28: cerebellum internal granule cell layer	Weak		4F , 4F'

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		4J

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	5B	Expression was uniform throughout the germinal zone.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	5G	Expression was uniform throughout the germinal zone.
TS20: dorsal root ganglion	Absent		5G
TS20: sympathetic ganglion	Absent		5G

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Present	Regionally restricted	6B	Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Much lower levels were noted in postmitotic precursors and differentiated cells in the inner neuroblast layer.
TS20: lens vesicle	Present		6B

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present	Regionally restricted	6E	Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Much lower levels were noted in postmitotic precursors and differentiated cells in the inner neuroblast layer.
TS23: lens	Present		6E

Structure	Level	Pattern	Image	Note
TS27: retina outer nuclear layer	Present	Regionally restricted	6H	Expression was detected in dividing progenitor cells in the outer neuroblast layer of the retina. Much lower levels were noted in postmitotic precursors.
TS27: retina inner nuclear layer	Weak	Regionally restricted	6H	Expression was low in differentiated cells in the inner neuroblast layer.
TS27: lens	Present		6H

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Strong	Regionally restricted	6K	Expression was high in differentiating photoreceptors in the outer nuclear layer.
TS28: retina inner nuclear layer	Present	Regionally restricted	6K	Expression was detected in a subset of cells in the inner nuclear layer.
TS28: retina ganglion cell layer	Present	Regionally restricted	6K	Expression was detected in a subset of cells in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Strong	Regionally restricted	6N	Expression was high in differentiating photoreceptors in the outer nuclear layer.
TS28: retina inner nuclear layer	Present	Regionally restricted	6N	Expression was detected in a subset of cells in the inner nuclear layer.
TS28: retina ganglion cell layer	Present	Regionally restricted	6N	Expression was detected in a subset of cells in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present	Widespread	7B	Expression was detected throughout the olfactory epithelium.
TS20: vomeronasal organ	Present		7B

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Present	Regionally restricted	7G	Expression was detected throughout the olfactory epithelium, with a concentration of transcripts in apical domains.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	8B	Expression was detected in the endocrine tissue of the Islets of Langerhans.
TS20: gut epithelium	Present		8B

Structure	Level	Pattern	Image	Note
TS20: gut epithelium	Present		8G

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	8K	Expression was detected in the endocrine tissue of the Islets of Langerhans.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Weak
TS17: facial ganglion	Weak
TS17: glossopharyngeal ganglion	Weak
TS17: trigeminal ganglion	Weak
TS17: vagus ganglion	Weak