Probe:	RIKEN clone F730215N04 subclone
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS23: male reproductive system	Present		GUDMAP:13613
TS23: genital tubercle of male	Present		GUDMAP:13613	Expression in the mesenchyme of glans and preputial swelling in a restricted pattern at base of the glans and in the core of the genital tubercle.
TS23: scrotal swelling	Present		GUDMAP:13613
TS23: male external genitalia	Present		GUDMAP:13613
TS23: scrotal swelling skin	Present	Regionally restricted	GUDMAP:13613	Expression appears to be regionalized to the basal epithelial cells.
TS23: mesenchyme of scrotal swelling	Absent		GUDMAP:13613
TS23: skin of glans of male genital tubercle	Absent		GUDMAP:13613
TS23: skin of male preputial swelling	Present		GUDMAP:13613
TS23: preputial swelling of male	Present		GUDMAP:13613
TS23: glans of male genital tubercle	Present		GUDMAP:13613
TS23: mesenchyme of glans of male genital tubercle	Present	Regionally restricted	GUDMAP:13613
TS23: epithelium of phallic urethra of male	Present		GUDMAP:13613	Expression detected in the proximal urethral epithelium/phallic urethral epithelium/urethral plate. The distal urethral epithelium was not examined.
TS23: urethral tube epithelium of male	Present		GUDMAP:13613
TS23: mesenchyme of male preputial swelling	Present	Regionally restricted	GUDMAP:13613

Structure	Level	Pattern	Image	Note
TS23: bladder	Present		GUDMAP:13614
TS23: urinary system	Present		GUDMAP:13614
TS23: urethra of male	Present		GUDMAP:13614
TS23: pelvic urethra of male	Present		GUDMAP:13614
TS23: phallic urethra of male	Present		GUDMAP:13614
TS23: urothelium of bladder	Present	Graded	GUDMAP:13614	May be graded expression in bladder epithelium - appears weaker in neck of bladder where ureter epithelium enters.
TS23: urethral plate epithelium of male	Present		GUDMAP:13614
TS23: epithelium of pelvic urethra of male	Present		GUDMAP:13614
TS23: lamina propria of bladder	Present		GUDMAP:13614
TS23: detrusor muscle of bladder	Absent		GUDMAP:13614
TS23: serosa of bladder	Absent		GUDMAP:13614
TS23: adventitia of bladder	Absent		GUDMAP:13614
TS23: vasculature of bladder	Absent		GUDMAP:13614
TS23: mesenchymal layer of pelvic urethra of male	Absent		GUDMAP:13614
TS23: muscle layer of pelvic urethra of male	Absent		GUDMAP:13614
TS23: urothelium of ureter	Absent		GUDMAP:13614
TS23: urothelium of bladder fundus	Present		GUDMAP:13614
TS23: urothelium of ventral bladder neck	Present		GUDMAP:13614

Structure	Level	Pattern	Image	Note
TS23: female reproductive system	Present		GUDMAP:13615
TS23: epithelium of phallic urethra of female	Present		GUDMAP:13615
TS23: urethral tube epithelium of female	Present		GUDMAP:13615
TS23: genital tubercle of female	Present		GUDMAP:13615
TS23: urethral plate epithelium of female	Ambiguous		GUDMAP:13615
TS23: preputial swelling of female	Ambiguous		GUDMAP:13615
TS23: skin of female preputial swelling	Ambiguous		GUDMAP:13615
TS23: glans of female genital tubercle	Ambiguous		GUDMAP:13615
TS23: genital tubercle mesenchyme of female	Ambiguous		GUDMAP:13615
TS23: labial swelling	Ambiguous		GUDMAP:13615
TS23: labial swelling skin	Ambiguous		GUDMAP:13615
TS23: mesenchyme of female preputial swelling	Absent		GUDMAP:13615
TS23: mesenchyme of glans of female genital tubercle	Absent		GUDMAP:13615
TS23: developing vasculature of female genital tubercle	Absent		GUDMAP:13615
TS23: mesenchyme of labial swelling	Absent		GUDMAP:13615
TS23: female external genitalia	Present		GUDMAP:13615	Weak expression in phallic urethra epithelium/urethral plate epithelium. Uncertain if signal detected in surface epithelia of genital tubercle and labioscrotal swelling is gene expression or background staining. Signal detected in skin surface epithelium of embryo appears to be true expression.

Structure	Level	Pattern	Image	Note
TS23: bladder	Present		GUDMAP:13616
TS23: urinary system	Present		GUDMAP:13616
TS23: urethra of female	Present		GUDMAP:13616
TS23: pelvic urethra of female	Present		GUDMAP:13616
TS23: phallic urethra of female	Present		GUDMAP:13616
TS23: urothelium of bladder	Present		GUDMAP:13616
TS23: urethral plate epithelium of female	Present		GUDMAP:13616
TS23: lamina propria of bladder	Present		GUDMAP:13616
TS23: detrusor muscle of bladder	Absent		GUDMAP:13616
TS23: serosa of bladder	Absent		GUDMAP:13616
TS23: adventitia of bladder	Absent		GUDMAP:13616
TS23: vasculature of bladder	Absent		GUDMAP:13616
TS23: mesenchymal layer of pelvic urethra of female	Absent		GUDMAP:13616
TS23: muscle layer of pelvic urethra of female	Absent		GUDMAP:13616
TS23: epithelium of pelvic urethra of female	Present		GUDMAP:13616