Gsc In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:119933 Lewis SL, et al., Genetic interaction of Gsc and Dkk1 in head morphogenesis of the mouse. Mech Dev. 2007 Feb;124(2):157-165
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5532320
Gene symbol:	Gsc
Gene name:	goosecoid homeobox

Reporter:	lacZ
	Direct Detection

Results

2F ©

Specimen 2F: embryonic day 8.5; Gsc^tm2Bhr/Gsc^tm2Bhr (more

)
Note: Double labeled: blue - Gsc; purple - Dkk1 (assay MGI:5532291).

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		2F

4D Fgf8 ©

Specimen 4D Fgf8: embryonic day 8.5; Dkk1^tm1Lmgd/Dkk1⁺, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)
Note: Early somite-stage embryo. Double labeled: blue - Gsc; purple - Fgf8 (assay MGI:5532336).

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		4D Fgf8

4D Hesx1 lateral view ©

Specimen 4D Hesx1 left: embryonic day 8.5; Dkk1^tm1Lmgd/Dkk1⁺, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)
Note: Early somite-stage embryo. Double labeled: blue - Gsc; purple - Hesx1 (assay MGI:5532337).

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		4D Hesx1 lateral view

4D Hesx1 en face view ©

Specimen 4D Hesx1 right: embryonic day 8.5; Dkk1^tm1Lmgd/Dkk1⁺, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)
Note: Early somite-stage embryo. Double labeled: blue - Gsc; purple - Hesx1 (assay MGI:5532337).

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		4D Hesx1 en face view

4D Six3 lateral view ©

Specimen 4D Six3 left: embryonic day 8.5; Dkk1^tm1Lmgd/Dkk1⁺, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)
Note: Early somite-stage embryo. Double labeled: blue - Gsc; purple - Six3 (assay MGI:5532338).

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		4D Six3 lateral view

4D Six3 en face view ©

Specimen 4D Six3 right: embryonic day 8.5; Dkk1^tm1Lmgd/Dkk1⁺, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)
Note: Early somite-stage embryo. Double labeled: blue - Gsc; purple - Six3 (assay MGI:5532338).

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		4D Six3 en face view

5A E8.0 Dkk1^+/+;Gsc^+/lacZ ©

Specimen 5A E8.0 Dkk1^+/+;Gsc^+/lacZ: embryonic day 8.0; Gsc^tm2Bhr/Gsc⁺ (more

)

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum	Present	Regionally restricted	5A E8.0 Dkk1^+/+;Gsc^+/lacZ	Expression was detected in the roof of the foregut.
TS12: mesenchyme derived from head mesoderm	Present	Regionally restricted	5A E8.0 Dkk1^+/+;Gsc^+/lacZ	Expression was detected in the prechordal plate.

5A E8.0 Dkk1^+/-;Gsc^+/lacZ ©

Specimen 5A E8.0 Dkk1^+/-;Gsc^+/lacZ: embryonic day 8.0; Dkk1^tm1Lmgd/Dkk1⁺, Gsc^tm2Bhr/Gsc⁺ (more

)

Structure	Level	Pattern	Image	Note
TS12: mesenchyme derived from head mesoderm	Ambiguous		5A E8.0 Dkk1^+/-;Gsc^+/lacZ	Expression is reduced or lost from the prechordal tissue.

5A E8.0 Dkk1^-/-;Gsc^+/lacZ ©

Specimen 5A E8.0 Dkk1^-/-;Gsc^+/lacZ: embryonic day 8.0; Dkk1^tm1Lmgd/Dkk1^tm1Lmgd, Gsc^tm2Bhr/Gsc⁺ (more

)

Structure	Level	Pattern	Image	Note
TS12: mesenchyme derived from head mesoderm	Ambiguous		5A E8.0 Dkk1^-/-;Gsc^+/lacZ	Expression is reduced or lost from the prechordal tissue.

5B E8.0 Dkk1^+/+;Gsc^lacZ/lacZ ©

Specimen 5B E8.0 Dkk^+/+;Gsc^lacZ/lacZ: embryonic day 8.0; Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS12: embryo endoderm	Strong	Regionally restricted	5B E8.0 Dkk1^+/+;Gsc^lacZ/lacZ	Expression was strong in the anterior axial mesendoderm.
TS12: embryo mesenchyme	Strong	Regionally restricted	5B E8.0 Dkk1^+/+;Gsc^lacZ/lacZ	Expression was strong in the anterior axial mesendoderm.
TS12: node	Present		5B E8.0 Dkk1^+/+;Gsc^lacZ/lacZ
TS12: notochordal plate	Present		5B E8.0 Dkk1^+/+;Gsc^lacZ/lacZ

5B E8.0 Dkk1^+/-;Gsc^lacZ/lacZ ©

Specimen 5B E8.0 Dkk^+/-;Gsc^lacZ/lacZ: embryonic day 8.0; Dkk1^tm1Lmgd/Dkk1⁺, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS12: embryo endoderm	Present	Regionally restricted	5B E8.0 Dkk1^+/-;Gsc^lacZ/lacZ	Expression was detected in the anterior axial mesendoderm.
TS12: embryo mesenchyme	Present	Regionally restricted	5B E8.0 Dkk1^+/-;Gsc^lacZ/lacZ	Expression was detected in the anterior axial mesendoderm.
TS12: node	Present		5B E8.0 Dkk1^+/-;Gsc^lacZ/lacZ
TS12: notochordal plate	Present		5B E8.0 Dkk1^+/-;Gsc^lacZ/lacZ

5B E8.0 Dkk1^-/-;Gsc^lacZ/lacZ left ©

Specimen 5B E8.0 Dkk^-/-;Gsc^lacZ/lacZ left: embryonic day 8.0; Dkk1^tm1Lmgd/Dkk1^tm1Lmgd, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS12: embryo endoderm	Present	Regionally restricted	5B E8.0 Dkk1^-/-;Gsc^lacZ/lacZ left	Ectopic expression was detected in the posterior mesendoderm.
TS12: embryo mesenchyme	Present	Regionally restricted	5B E8.0 Dkk1^-/-;Gsc^lacZ/lacZ left	Ectopic expression was detected in the posterior mesendoderm.

5B E8.0 Dkk1^-/-;Gsc^lacZ/lacZ right ©

Specimen 5B E8.0 Dkk^-/-;Gsc^lacZ/lacZ right: embryonic day 8.0; Dkk1^tm1Lmgd/Dkk1^tm1Lmgd, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS12: embryo endoderm	Present	Regionally restricted	5B E8.0 Dkk1^-/-;Gsc^lacZ/lacZ right	Ectopic expression was detected in the posterior mesendoderm.
TS12: embryo mesenchyme	Present	Regionally restricted	5B E8.0 Dkk1^-/-;Gsc^lacZ/lacZ right	Ectopic expression was detected in the posterior mesendoderm.

5A E8.5 Dkk1^+/+;Gsc^+/lacZ ©

Specimen 5A E8.5 Dkk1^+/+;Gsc^+/lacZ: embryonic day 8.5; Gsc^tm2Bhr/Gsc⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present	Regionally restricted	5A E8.5 Dkk1^+/+;Gsc^+/lacZ	Expression was detected in the roof of the foregut.
TS13: mesenchyme derived from head mesoderm	Present	Regionally restricted	5A E8.5 Dkk1^+/+;Gsc^+/lacZ	Expression was detected in the prechordal plate.

5A E8.5 Dkk1^+/-;Gsc^+/lacZ ©

Specimen 5A E8.5 Dkk1^+/-;Gsc^+/lacZ: embryonic day 8.5; Dkk1^tm1Lmgd/Dkk1⁺, Gsc^tm2Bhr/Gsc⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: mesenchyme derived from head mesoderm	Ambiguous		5A E8.5 Dkk1^+/-;Gsc^+/lacZ	Expression is reduced or lost from the prechordal tissue.

5A E8.5 Dkk1^-/-;Gsc^+/lacZ ©

Specimen 5A E8.5 Dkk1^-/-;Gsc^+/lacZ: embryonic day 8.5; Dkk1^tm1Lmgd/Dkk1^tm1Lmgd, Gsc^tm2Bhr/Gsc⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: mesenchyme derived from head mesoderm	Ambiguous		5A E8.5 Dkk1^-/-;Gsc^+/lacZ	Expression is reduced or lost from the prechordal tissue.

5B E8.5 Dkk1^+/+;Gsc^lacZ/lacZ ©

Specimen 5B E8.5 Dkk^+/+;Gsc^lacZ/lacZ: embryonic day 8.5; Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present	Regionally restricted	5B E8.5 Dkk1^+/+;Gsc^lacZ/lacZ	Expression was detected along the entire midline in the notochord.
TS13: embryo endoderm	Present	Regionally restricted	5B E8.5 Dkk1^+/+;Gsc^lacZ/lacZ	Expression was detected in the most rostral part of the axial mesendoderm.
TS13: embryo mesenchyme	Present	Regionally restricted	5B E8.5 Dkk1^+/+;Gsc^lacZ/lacZ	Expression was detected in the most rostral part of the axial mesendoderm.

5B E8.5 Dkk1^+/-;Gsc^lacZ/lacZ ©

Specimen 5B E8.5 Dkk^+/-;Gsc^lacZ/lacZ: embryonic day 8.5; Dkk1^tm1Lmgd/Dkk1⁺, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		5B E8.5 Dkk1^+/-;Gsc^lacZ/lacZ
TS13: embryo endoderm	Present	Regionally restricted	5B E8.5 Dkk1^+/-;Gsc^lacZ/lacZ	Expression was detected in the most rostral part of the axial mesendoderm.
TS13: embryo mesenchyme	Present	Regionally restricted	5B E8.5 Dkk1^+/-;Gsc^lacZ/lacZ	Expression was detected in the most rostral part of the axial mesendoderm.

5B E8.5 Dkk1^-/-;Gsc^lacZ/lacZ left ©

Specimen 5B E8.5 Dkk^-/-;Gsc^lacZ/lacZ left: embryonic day 8.5; Dkk1^tm1Lmgd/Dkk1^tm1Lmgd, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present	Regionally restricted	5B E8.5 Dkk1^-/-;Gsc^lacZ/lacZ left	Expression was lost from the prechordal region.
TS13: embryo endoderm	Weak	Regionally restricted	5B E8.5 Dkk1^-/-;Gsc^lacZ/lacZ left	Expression was markedly reduced in the most rostral part of the axial mesendoderm.
TS13: embryo mesenchyme	Weak	Regionally restricted	5B E8.5 Dkk1^-/-;Gsc^lacZ/lacZ left	Expression was markedly reduced in the most rostral part of the axial mesendoderm.

Specimen 5B E8.5 Dkk^-/-;Gsc^lacZ/lacZ right: embryonic day 8.5; Dkk1^tm1Lmgd/Dkk1^tm1Lmgd, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present	Regionally restricted	5B E8.5 Dkk1^-/-;Gsc^lacZ/lacZ right	Expression was lost from the prechordal region.
TS13: embryo endoderm	Weak	Regionally restricted	5B E8.5 Dkk1^-/-;Gsc^lacZ/lacZ right	Expression was markedly reduced in the most rostral part of the axial mesendoderm.
TS13: embryo mesenchyme	Weak	Regionally restricted	5B E8.5 Dkk1^-/-;Gsc^lacZ/lacZ right	Expression was markedly reduced in the most rostral part of the axial mesendoderm.

Specimen 5A E9.5 Dkk1^+/+;Gsc^+/lacZ: embryonic day 9.5; Gsc^tm2Bhr/Gsc⁺ (more

)

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from head mesoderm	Present	Regionally restricted	5A E9.5 Dkk1^+/+;Gsc^+/lacZ	Expression was detected in the prechordal plate.
TS15: foregut	Present	Regionally restricted	5A E9.5 Dkk1^+/+;Gsc^+/lacZ	Expression was detected in the roof of the foregut.

Specimen 5A E9.5 Dkk1^+/-;Gsc^+/lacZ: embryonic day 9.5; Dkk1^tm1Lmgd/Dkk1⁺, Gsc^tm2Bhr/Gsc⁺ (more

)

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from head mesoderm	Ambiguous		5A E9.5 Dkk1^+/-;Gsc^+/lacZ	Expression is reduced or lost from the prechordal tissue.

Specimen 5A E9.5 Dkk1^-/-;Gsc^+/lacZ: embryonic day 9.5; Dkk1^tm1Lmgd/Dkk1^tm1Lmgd, Gsc^tm2Bhr/Gsc⁺ (more

)

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from head mesoderm	Ambiguous		5A E9.5 Dkk1^-/-;Gsc^+/lacZ	Expression is reduced or lost from the prechordal tissue.

Specimen 5B E9.5 Dkk^+/+;Gsc^lacZ/lacZ: embryonic day 9.5; Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS15: embryo endoderm	Present	Regionally restricted	5B E9.5 Dkk1^+/+;Gsc^lacZ/lacZ	Expression was detected in the axial mesendoderm.
TS15: embryo mesenchyme	Present	Regionally restricted	5B E9.5 Dkk1^+/+;Gsc^lacZ/lacZ	Expression was detected in the axial mesendoderm.

Specimen 5B E9.5 Dkk^+/-;Gsc^lacZ/lacZ: embryonic day 9.5; Dkk1^tm1Lmgd/Dkk1⁺, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS15: embryo endoderm	Present	Regionally restricted	5B E9.5 Dkk1^+/-;Gsc^lacZ/lacZ	Expression was detected in the axial mesendoderm.
TS15: embryo mesenchyme	Present	Regionally restricted	5B E9.5 Dkk1^+/-;Gsc^lacZ/lacZ	Expression was detected in the axial mesendoderm.

Specimen 5B E9.5 Dkk^-/-;Gsc^lacZ/lacZ left: embryonic day 9.5; Dkk1^tm1Lmgd/Dkk1^tm1Lmgd, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS15: embryo endoderm	Absent		5B E9.5 Dkk1^-/-;Gsc^lacZ/lacZ left	Expression was absent from the axial mesendoderm.
TS15: embryo mesenchyme	Absent		5B E9.5 Dkk1^-/-;Gsc^lacZ/lacZ left	Expression was absent from the axial mesendoderm.

Specimen 5B E9.5 Dkk^-/-;Gsc^lacZ/lacZ right: embryonic day 9.5; Dkk1^tm1Lmgd/Dkk1^tm1Lmgd, Gsc^tm2Bhr/Gsc^tm2Bhr (more

)

Structure	Level	Pattern	Image	Note
TS15: embryo endoderm	Absent		5B E9.5 Dkk1^-/-;Gsc^lacZ/lacZ right	Expression was absent from the axial mesendoderm.
TS15: embryo mesenchyme	Absent		5B E9.5 Dkk1^-/-;Gsc^lacZ/lacZ right	Expression was absent from the axial mesendoderm.

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