Tg(Wnk4*Q562E)#Rpl
Transgene Detail
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| Symbol: |
Tg(Wnk4*Q562E)#Rpl |
| Name: |
transgene insertion, Richard P Lifton |
| MGI ID: |
MGI:5525135 |
| Synonyms: |
TgWNK4Q562E, Wnk4PHAII |
| Transgene: |
Tg(Wnk4*Q562E)#Rpl Location: unknown
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| Alliance: |
Tg(Wnk4*Q562E)#Rpl page
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| Strain of Origin: |
(C3H x C57BL/6)F1
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Wnk4*Q562E)#Rpl expresses
1 gene
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Mutation details: This transgene consists of a 36.8-kb SwaI fragment of the BAC clone RP23-551L11 (from 129S6/SvEvTac) that contains the complete Wnk4 transcription unit plus 9 kb of 5' flanking and 11.5 kb of 3' flanking sequence. Two substitutions were introduced: one that creates the pseudohypoaldosteronism type II (PHAII) amino acid substitution Q562E and a synonymous substitution at codon 563, creating a new Bsp1286I cleavage site. Fluorescence in situ hybridization showed a single site of insertion. Transgene expression was detected in heart, kidney, testis, brain, and lung but not in liver or spleen. RT-PCR showed that total amount of mRNA is increased by about 2-fold. The pound symbol (#) is used when line is not specified and/or lines are pooled.
(J:113094)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:113094 Lalioti MD, et al., Wnk4 controls blood pressure and potassium homeostasis via regulation of mass and activity of the distal convoluted tubule. Nat Genet. 2006 Oct;38(10):1124-1132 |
| All: |
8 reference(s) |
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Analysis Tools
