Cradd In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:96712 Motaln H, et al., In situ analysis of Raidd-beta-galactosidase fusion gene expression in transgenic mouse midgestation embryos. Transgenic Res. 2005 Feb;14(1):27-40
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5524115
Gene symbol:	Cradd
Gene name:	CASP2 and RIPK1 domain containing adaptor with death domain

Reporter:	lacZ
	Direct Detection
Assay notes:	Embryos were pre-fixed in 0.2% glutaraldehyde and post-fixed over night in 4% paraformaldehyde,

Results

Specimen 3b E8.5: embryonic day 8.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		3b +/- 8.5

Specimen 3b E9.5: embryonic day 9.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS15: heart	Present		3b +/- 9.5
TS15: trunk	Present	Regionally restricted	3b +/- 9.5	Expression is in the pericardial region.
TS15: limb	Absent		3b +/- 9.5
TS15: myotome	Absent		3b +/- 9.5
TS15: tail	Absent		3b +/- 9.5

Specimen 3b E10.5: embryonic day 10.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS17: heart	Strong		3b +/- 10.5
TS17: trunk	Strong	Regionally restricted	3b +/- 10.5	Expression is in the pericardial region and at the whole abdominal side of the embryo just beneath the forelimb bud.
TS17: 1st branchial arch	Present	Regionally restricted	3b +/- 10.5	Expression is ventral.
TS17: 2nd branchial arch	Present	Regionally restricted	3b +/- 10.5	Expression is ventral.
TS17: limb	Absent		3b +/- 10.5
TS17: myotome	Absent		3b +/- 10.5
TS17: tail	Absent		3b +/- 10.5

Specimen 3b E11.5: embryonic day 11.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS19: embryo	Present		3b +/- 11.5
TS19: brain	Present	Diffuse	3b +/- 11.5	Expression is more concentrated around the developing optic vesicle.
TS19: eye	Present		3b +/- 11.5	Expression is in and around the developing optic vesicle.
TS19: limb	Absent		3b +/- 11.5
TS19: myotome	Absent		3b +/- 11.5
TS19: tail	Absent		3b +/- 11.5

Specimen 3b E12.5: embryonic day 12.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS20: embryo	Present		3b +/- 12.5
TS20: brain	Present	Diffuse	3b +/- 12.5	Expression is more concentrated around the developing optic vesicle.
TS20: eye	Present		3b +/- 12.5	Expression is in and around the developing optic vesicle.
TS20: limb	Absent		3b +/- 12.5
TS20: myotome	Absent		3b +/- 12.5
TS20: tail	Absent		3b +/- 12.5

Specimen Table 1 E8.5: embryonic day 8.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS13: eye	Absent
TS13: future hindbrain	Absent
TS13: future spinal cord	Absent
TS13: genitourinary system	Absent
TS13: heart	Absent
TS13: inner ear	Absent
TS13: midgut	Absent
TS13: trunk	Absent

Specimen Table 1 E9.5: embryonic day 9.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS15: heart	Present
TS15: foregut	Absent
TS15: future hindbrain	Absent
TS15: future spinal cord	Absent
TS15: inner ear	Absent
TS15: lens placode	Absent
TS15: mesonephros	Absent
TS15: midgut	Absent

Specimen Table 1 E10.5: embryonic day 10.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present
TS17: heart	Present
TS17: mesonephros	Present
TS17: stomach	Present
TS17: eye	Absent
TS17: hindbrain	Absent
TS17: inner ear	Absent
TS17: midgut	Absent

Specimen Table 1 E11.5: embryonic day 11.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present
TS19: heart	Present
TS19: metanephros	Present
TS19: midgut	Present
TS19: stomach	Present
TS19: hindbrain	Absent
TS19: inner ear	Absent
TS19: lens vesicle	Absent

Specimen Table 1 E12.5: embryonic day 12.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS20: heart	Present
TS20: hindbrain	Present
TS20: inner ear	Present
TS20: lens vesicle	Present
TS20: metanephros	Present
TS20: midgut	Present
TS20: spinal cord	Present
TS20: stomach	Present

Specimen 4a 10.5: embryonic day 10.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS17: common atrial chamber left part	Present		4a +/- E10.5 heart
TS17: primitive ventricle	Present		4a +/- E10.5 heart

Specimen 4a 11.5: embryonic day 11.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS19: heart atrium	Present		4a +/- E11.5 heart
TS19: heart ventricle	Present		4a +/- E11.5 heart

Specimen 4b 11.5: embryonic day 11.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS19: mesonephric tubule	Strong		4b +/- E11.5 kidney
TS19: nephric duct	Present	Regionally restricted	4b +/- E11.5 kidney	Expression is in the epithelium.

Specimen 4b 12.5: embryonic day 12.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS20: mesonephric tubule	Strong		4b +/- E12.5 kidney
TS20: nephric duct	Present	Regionally restricted	4b +/- E12.5 kidney	Expression is in the epithelium.

Specimen 4c: embryonic day 12.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS20: lens vesicle equatorial epithelium	Strong		4c +/-

Specimen 4d: embryonic day 12.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS20: cochlea	Present	Regionally restricted	4d +/-	Expression is in the medio-ventral side of the cochlea.
TS20: semicircular canal epithelium	Present	Regionally restricted	4d +/-	Expression is in the ventral epithelium of the forming semicircular canals.
TS20: inner ear mesenchyme	Absent		4d +/-

Specimen 4e: embryonic day 12.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS20: hindbrain roof	Strong	Regionally restricted	4e +/-	Expression is mostly restricted to a small group of ependymal cells, attached to the roof.
TS20: hindbrain	Weak	Regionally restricted	4e +/-	Expression is in the ependymal cells in the most ventral part of the fourth ventricle cavity.

Specimen not shown E10.5: embryonic day 10.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern
TS17: atrioventricular canal	Present	Patchy
TS17: bulbus cordis	Present	Patchy
TS17: outflow tract	Present	Patchy
TS17: endocardial cushion	Absent

Specimen not shown E12.5: embryonic day 12.5; Cradd^tm1Shor/Cradd⁺ (more

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Structure	Level	Pattern	Image	Note
TS20: lung	Weak
TS20: liver	Absent
TS20: pancreas	Absent

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