Antibody:	anti-tenascin
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: cochlea	Present	Regionally restricted	1A	Expression was detected in radially oriented stripes that fanned out to form a diffuse spiral band in the spiral lamina. Puncta of immunoreactivity were detected in the organ of Corti along the inner pillar cells.

Structure	Level	Pattern	Image	Note
TS24: cochlea	Present	Regionally restricted	1B	The radial pattern of expression in the spiral lamina was more elongated than at E14.5, and the spiral pattern was not as prominent.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	1D	Expression accumulates around cells in both the inner and outer hair cell regions.

Structure	Level	Pattern	Image	Note
TS22: cochlea	Present	Regionally restricted	1E	Expression was detected in the inner and outer hair cell regions in the basal turn of the organ of Corti. Small and large puncta of label were beneath outer hair cells, and Deiters' cells were outlined with label.

Structure	Level	Pattern	Image	Note
TS22: cochlea	Present	Regionally restricted	1F	There was a limited amount of labeling in the inner hair cell region of the apical turn of the organ of Corti. No label was in the outer hair cell region.

Structure	Level	Pattern	Image	Note
TS24: organ of Corti	Present	Regionally restricted	2A	The strongest labeling in the inner hair cell region was between the inner sensory cells and the inner pillar cells. Large accumulations of label were detected beneath the synaptic region of outer hair cells.

Structure	Level	Pattern	Image	Note
TS24: organ of Corti	Present	Regionally restricted	2B	Strongest label in the inner hair cell (IHC) region was between inner sensory and inner pillar cells. Little label was on the modiolar side of IHC. Label was beneath the synaptic region of outer hair cells. Faint label was at the apical end of hair cells.

Structure	Level	Pattern	Image	Note
TS24: organ of Corti	Present	Regionally restricted	2C	Strongest label in the inner hair cell (IHC) region was between inner sensory and inner pillar cells. Small puncta of label were beneath outer hair cells in the apical turn of the organ of Corti. The lateral edges of Deiters' cells were outlined.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	2D	Strongest label in the inner hair cell (IHC) region was between inner sensory and inner pillar cells. Label was beneath the synaptic region of outer hair cells. Faint label was at the apical end of hair cells. Label continued to surround Deiters' cells.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	2E	The strongest labeling in the inner hair cell region was between the inner sensory cells and the inner pillar cells. After birth fewer and fewer labeled puncta can be detected around the Deiters' cells.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	2F	Strongest label in the inner hair cell (IHC) region was between inner sensory and inner pillar cells. Label was on the modiolar side of IHC in the basal turn. After birth fewer and fewer labeled puncta can be detected around the Deiters' cells.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	2G	At a level closer to the basilar membrane, label dotted the region between the inner hair and inner pillar cells. After birth fewer and fewer labeled puncta can be detected around the Deiters' cells.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	2H	At a slightly higher level than in 2G, the apical ends of the pillar cells were labeled. Outer hair cells were unlabeled. Labeling was on the modiolar side of inner hair cells in the basal turn.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	3A	In the apical turn, labeling increases around Deiters' cells.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	3B	Large accumulations of immunolabel are detected beneath the synaptic region of outer hair cells in the apical turn. Labeling increases around Deiters' cells.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	3C	Labeling remains on the modiolar side of inner hair cells in the apical turn. Only sparse labeling around Deiters' cells can be detected in the apical turn, although pillar cells are labeled.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	3D	Only sparse labeling around Deiters' cells can be detected in the apical turn, although pillar cells are labeled.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	3E	Immunoreactivity in the inner hair cell region is confined to widely scattered, small puncta beneath the inner hair cell.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	3F	Immunoreactivity in the organ of Corti has disappeared except for widely scattered, small puncta beneath the inner hair cell.
TS28: basilar membrane	Strong	Regionally restricted	3F	Strong labeling was detected along the basilar membrane.

Structure	Level	Pattern	Image	Note
TS25: organ of Corti	Strong	Regionally restricted	4A	Strong immunoreactivity was detected in rounded-up inner hair cells in the basal turn.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	4B	Immunolabel fills the entire cytoplasm of the cell.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	4C	Immunolabel fills the entire cytoplasm of the inner hair cell. Expression remains along the inner pillar cells beneath the outer hair cells.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	4D	Immunoreactivity was detected at the apical ends of the hair cells.

Structure	Level	Pattern	Image	Note
TS24: organ of Corti	Present	Regionally restricted	4E	Apical ends of inner hair cells were labeled.