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Gene Expression Data
Assay Details
Assay
Reference: J:135584 Fedtsova N, et al., Regulation of the development of tectal neurons and their projections by transcription factors Brn3a and Pax7. Dev Biol. 2008 Apr 1;316(1):6-20
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5516015
Gene symbol: Pou4f1
Gene name: POU domain, class 4, transcription factor 1
Reporter: lacZ
Antibody: anti-B-galactosidase (Biogenesis, 4600-1409)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 5C/D: embryonic day 16.5; Pou4f1tm1Et/Pou4f1+ (more )
Structure Level Pattern Image Note
TS24: midbrain tegmentum Present Regionally restricted 5C , 5D Expressed in the rubrospinal tract but not the decussating fibers of the crossed tectobulbar.

Specimen 5E/F: embryonic day 16.5; Pou4f1tm1Et/Pou4f1+ (more )
Structure Level Pattern Image Note
TS24: midbrain tegmentum Present Regionally restricted 5E , 5F Expressed in the ipsilateral tectopontine tract (uncrossed tectobulbar tract), but not in the tectospinal tract.

Specimen 5H: postnatal day 10; Pou4f1tm1Et/Pou4f1+ (more )
Structure Level Pattern Image Note
TS28: midbrain Present Regionally restricted 5L , 5K , 5H , 5I Expressed in the red nucleus and ipsilateral tectopontine tract, but not the the decussating fibers of the tectospinal tract.
TS28: red nucleus Present 5H , 5K , 5L
TS28: habenula Present Regionally restricted 5K , 5H , 5L Expressed in fasciculus retroflexus.
TS28: midbrain tegmentum Present Regionally restricted 5H , 5I Expressed in the uncrossed tectobulbar tract which appears as diffuse fibers in the lateral tegmentum.

Specimen S2A: embryonic day 18.5; Pou4f1tm1Et/Pou4f1+ (more )
Structure Level Pattern Image Note
TS26: midbrain tegmentum Present Regionally restricted S2A Expressed in the red nucleus, but not the fibers of the crossed tectobulbar tract.

Specimen S2B: embryonic day 18.5; Pou4f1tm1Et/Pou4f1tm1Nat (more )
Structure Level Pattern Image Note
TS26: midbrain tegmentum Present Regionally restricted S2B Expressed in the red nucleus, but not the fibers of the crossed tectobulbar tract.

Specimen S2C/G/H: embryonic day 18.5; Pou4f1tm1Et/Pou4f1+ (more )
Structure Level Pattern Image Note
TS26: midbrain tegmentum Present Regionally restricted S2C , S2H , S2G Expressed in the red nucleus and ipsilateral tectopontine tract, but not the the decussating fibers of the tectospinal tract.

Specimen S2D/I/J: embryonic day 18.5; Pou4f1tm1Et/Pou4f1tm1Nat (more )
Structure Level Pattern Image Note
TS26: midbrain tegmentum Present Regionally restricted S2J , S2I , S2D Expressed in the red nucleus and ipsilateral tectopontine tract, but not the the decussating fibers of the tectospinal tract. Reduced extent of the red nucleus and ventral tegmental decussation, and the rubrospinal tract was nearly absent.

Specimen S2E: embryonic day 18.5; Pou4f1tm1Et/Pou4f1+ (more )
Structure Level Pattern Image Note
TS26: midbrain tegmentum Present Regionally restricted S2E Expressed in the red nucleus and ipsilateral tectopontine tract, but not the the decussating fibers of the tectospinal tract.

Specimen S2F: embryonic day 18.5; Pou4f1tm1Et/Pou4f1tm1Nat (more )
Structure Level Pattern Image Note
TS26: midbrain tegmentum Present Regionally restricted S2F Expressed in the red nucleus and ipsilateral tectopontine tract, but not the the decussating fibers of the tectospinal tract. The rubrospinal tract is absent.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory