Gene Expression Data
Assay Details
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Assay
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Reference:
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J:49126
Zachgo J, et al., Genetic interactions suggest that Danforth's short tail (Sd) is a gain-of-function mutation. Dev Genet. 1998;23(1):86-96
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5510848
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Gene symbol:
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Pax1
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Gene name:
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paired box 1
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Probe:
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Pax1 probe21
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 11.5; Etl4Gt(6LSN)6029Gos/Etl4 +, Etn2Sd/Etn2 +
(more  )
Note: Also stained for T (assay MGI:5510855). In this specimen, the two mutations are on the same chromosome after recombination (cis).
Specimen
6A:
(close  )
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Genetic Background:
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involves: 129/Sv * 129S2/SvPas * C57BL/6 * NMRI
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Mutant Allele(s):
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Etl4Gt(6LSN)6029Gos/Etl4+
Etn2Sd/Etn2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Also stained for T (assay MGI:5510855). In this specimen, the two mutations are on the same chromosome after recombination (cis).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail |
Present |
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6A
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Reduced expression compared with the wild type.
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Specimen
6B:
embryonic day 11.5; Etl4Gt(6LSN)6029Gos/Etl4 +, Etn2Sd/Etn2 +
(more )
Note: Also stained for T (assay MGI:5510855). In this specimen, the two mutations are on different chromosomes (trans).
Specimen
6B:
(close )
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Genetic Background:
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involves: 129/Sv * 129S2/SvPas * C57BL/6 * NMRI
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Mutant Allele(s):
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Etl4Gt(6LSN)6029Gos/Etl4+
Etn2Sd/Etn2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Also stained for T (assay MGI:5510855). In this specimen, the two mutations are on different chromosomes (trans).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail |
Absent |
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6B
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Specimen
6C:
embryonic day 11.5
(more )
Note: Also stained for T (assay MGI:5510855).
Specimen
6C:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Also stained for T (assay MGI:5510855).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail |
Present |
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6C
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129/Sv * 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Etl4Gt(6LSN)6029Gos/Etl4Gt(6LSN)6029Gos
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail |
Present |
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Same as the wild type.
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Analysis Tools