Probe:	Arx probe2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Present	Regionally restricted	1A	Expression is in the pallium.
TS17: diencephalon lateral wall ventricular layer	Present	Regionally restricted	1A	Expression is in the eminentia thalami and ventral thalamus.
TS17: telencephalon	Present	Regionally restricted	1A	Expression is in the mantle zone of the lateral ganglionic eminence and in the subventricular zone and mantle of the medial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Present	Regionally restricted	1B	Expression is in the pallium.
TS17: diencephalon lateral wall ventricular layer	Present	Regionally restricted	1B	Expression is in the eminentia thalami and ventral thalamus.
TS17: telencephalon	Present	Regionally restricted	1B	Expression is in the mantle zone of the lateral ganglionic eminence and in the subventricular zone and mantle of the medial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Present	Regionally restricted	1C	Expression is in the pallium.
TS17: diencephalon lateral wall ventricular layer	Present	Regionally restricted	1C	Expression is in the eminentia thalami and ventral thalamus.

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Present	Regionally restricted	1D	Expression is in the pallium.
TS17: diencephalon lateral wall ventricular layer	Present	Regionally restricted	1D	Expression is in the eminentia thalami and ventral thalamus.

Structure	Level	Pattern	Image	Note
TS17: diencephalon lateral wall ventricular layer	Present	Regionally restricted	1E	Expression is in the eminentia thalami and ventral thalamus.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		1F
TS20: telencephalon ventricular layer	Present		1F

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		1G
TS20: telencephalon ventricular layer	Present		1G

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		1H
TS20: hypothalamus	Present		1H
TS20: telencephalon ventricular layer	Present		1H
TS20: diencephalon	Present	Regionally restricted	1H	Expression is in the eminentia thalami.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		1I
TS20: hypothalamus	Present		1I
TS20: telencephalon ventricular layer	Present		1I

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		1J
TS20: telencephalon ventricular layer	Present		1J

Structure	Level	Pattern	Image	Note
TS24: telencephalon mantle layer	Present	Regionally restricted	1K	Expression is in the subpallium.
TS24: telencephalon ventricular layer	Present	Regionally restricted	1K	Expression is in the subpallium.
TS24: telencephalon	Present	Regionally restricted	1K	Expression is in the subventricular zone of the subpallium.

Structure	Level	Pattern	Image	Note
TS24: telencephalon mantle layer	Present	Regionally restricted	1L	Expression is in the subpallium.
TS24: telencephalon ventricular layer	Present	Regionally restricted	1L	Expression is in the subpallium.
TS24: telencephalon	Present	Regionally restricted	1L	Expression is in the subventricular zone of the subpallium.

Structure	Level	Pattern	Image	Note
TS24: telencephalon mantle layer	Present	Regionally restricted	1M	Expression is in the subpallium.
TS24: telencephalon ventricular layer	Present	Regionally restricted	1M	Expression is in the subpallium.
TS24: telencephalon	Present	Regionally restricted	1M	Expression is in the subventricular zone of the subpallium.
TS24: hypothalamus	Present	Regionally restricted	1M	Expression is in the anterior and the dorsomedial hypothalamic nuclei.

Structure	Level	Pattern	Image	Note
TS24: telencephalon mantle layer	Present	Regionally restricted	1N
TS24: telencephalon ventricular layer	Present	Regionally restricted	1N
TS24: telencephalon	Present	Regionally restricted	1N	Expression is in the subventricular zone of the subpallium.
TS24: cerebral cortex intermediate zone	Present	Regionally restricted	1O , 1N	Expression is in tangentially migrating neurons.
TS24: cerebral cortex mantle layer	Present	Regionally restricted	1O , 1N	Expression is in tangentially migrating neurons.
TS24: cerebral cortex subventricular zone	Present	Regionally restricted	1O , 1N	Expression is in tangentially migrating neurons.
TS24: cortical plate	Present	Regionally restricted	1O , 1N	Expression is in tangentially migrating neurons.
TS24: thalamus	Present	Regionally restricted	1N	Expression is in the ventral thalamus and includes nuclei developing from these progenitor zones, including the ventral lateral geniculate nucleus, the thalamic reticular nucleus, and the zona incerta.
TS24: zona incerta	Present		1N

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Present	Regionally restricted	1P	Expression is in the ventricular zone and subventricular zone and in differentiated interneurons in the granular and periglomerular layers.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Regionally restricted	1Q	Expression is in the ventricular zone and subventricular zone.
TS27: main olfactory bulb glomerular layer	Present	Regionally restricted	1Q	Expression is in differentiated interneurons in the granular and periglomerular layers.
TS27: main olfactory bulb granule cell layer	Present	Regionally restricted	1Q	Expression is in differentiated interneurons in the granular and periglomerular layers.

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Present		2A Arx
TS21: telencephalon mantle layer	Present	Regionally restricted	2A Arx	Expression is in the subpallial areas.
TS21: telencephalon ventricular layer	Present	Regionally restricted	2A Arx	Expression is in the subpallial areas.
TS21: telencephalon	Present	Regionally restricted	2A Arx	Expression is in the septum.

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Present		2B Arx

Structure	Level	Pattern	Image	Note
TS21: thalamus	Present	Regionally restricted	2C Arx	Expression is in the ventral thalamus.
TS21: ganglionic eminence	Present		2C Arx
TS21: hypothalamus	Present		2C Arx

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	6A	Expression is in the lateral and medial ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	6A'	Expression in the lateral and medial ganglionic eminences is reduced.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	6B	Expression is in the lateral and medial ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	6B'	Expression in the lateral and medial ganglionic eminences is reduced.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present		6C	Expression is in the ventral thalamus and hypothalamus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Weak	Regionally restricted	6C'	Expression is in the ventral thalamus and hypothalamus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Present	Regionally restricted	6D	Expression is in the ventral thalamus.
TS19: diencephalon lateral wall ventricular layer	Present	Regionally restricted	6D	Expression is in the ventral thalamus.
TS19: diencephalon	Present	Regionally restricted	6D	Expression is in the ventral thalamus and hypothalamic nuclei.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Weak	Regionally restricted	6D'	Expression is in the ventral thalamus.
TS19: diencephalon lateral wall ventricular layer	Present	Regionally restricted	6D'	Expression in the ventral thalamus is not greatly altered in this mutant.
TS19: diencephalon	Trace	Regionally restricted	6D'	Expression is in the ventral thalamus and hypothalamic nuclei.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Present		6E
TS21: medial ganglionic eminence	Present		6E
TS21: telencephalon	Present	Regionally restricted	6E	Expression is in the septum.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Trace		6E'
TS21: medial ganglionic eminence	Trace		6E'
TS21: telencephalon	Trace	Regionally restricted	6E'	Expression is in the septum.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Present		6F
TS21: medial ganglionic eminence	Present		6F
TS21: telencephalon	Present	Regionally restricted	6F	Expression is in the septum.
TS21: corpus striatum	Present		6F

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Trace		6F'
TS21: medial ganglionic eminence	Trace		6F'
TS21: telencephalon	Trace	Regionally restricted	6F'	Expression is in the septum.
TS21: corpus striatum	Trace		6F'

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Present	Regionally restricted	6G	Expression is in the anterior and dorsomedial hypothalamic nuclei.

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Trace	Regionally restricted	6G'	Expression is in the anterior and dorsomedial hypothalamic nuclei.

Structure	Level	Pattern	Image	Note
TS21: thalamus	Present	Regionally restricted	6H	Expression is in the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS21: thalamus	Weak	Regionally restricted	6H'	Expression is in the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Present		6I
TS24: medial ganglionic eminence	Present		6I

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Weak		6I'
TS24: medial ganglionic eminence	Weak		6I'

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Present		6J
TS24: medial ganglionic eminence	Present		6J

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Weak		6J'
TS24: medial ganglionic eminence	Weak		6J'

Structure	Level	Pattern	Image	Note
TS24: thalamus	Present	Regionally restricted	6K	Expression is in the diencephalic nuclei including the ventral lateral geniculate nucleus and the reticular nucleus.
TS24: hypothalamus	Present	Regionally restricted	6K	Expression is in the anterior and dorsomedial hypothalamic nuclei.

Structure	Level	Pattern	Image	Note
TS24: thalamus	Trace	Regionally restricted	6K'	Expression is in the diencephalic nuclei including the ventral lateral geniculate nucleus and the reticular nucleus.
TS24: hypothalamus	Trace	Regionally restricted	6K'	Expression is in the anterior and dorsomedial hypothalamic nuclei.

Structure	Level	Pattern	Image	Note
TS24: thalamus	Present	Regionally restricted	6L	Expression is in the diencephalic nuclei including the ventral lateral geniculate nucleus and the reticular nucleus.

Structure	Level	Pattern	Image	Note
TS24: thalamus	Trace	Regionally restricted	6L'	Expression is in the diencephalic nuclei including the ventral lateral geniculate nucleus and the reticular nucleus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex mantle layer	Present	Single cells	6M	Expression is in interneurons.
TS24: cerebral cortex subventricular zone	Present	Single cells	6M	Expression is in interneurons.
TS24: cortical plate	Present	Single cells	6M	Expression is in interneurons.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex mantle layer	Absent		6M'
TS24: cerebral cortex subventricular zone	Absent		6M'
TS24: cortical plate	Absent		6M'

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Single cells	6O	Expression is in interneurons.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Absent		6O'