Antibody:	Anti-HNF-6 (H-100) sc-13050
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS19: retina	Present			Expression was detected in the nuclei.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	1A	Expression was detected in both the ganglion cell layer and the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	1B	Expression was detected in both the ganglion cell layer and the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS24: retina	Present	Regionally restricted	1C	Expression was detected mainly in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		1D	Expression in the ganglion cell layer was still high at P0.
TS27: retina layer	Present	Regionally restricted	1D	Expression was detected in a row of regularly spaced cells located in the outer part of the retina.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Weak		1E	Expression in the ganglion cell layer decreased markedly at P5.
TS28: retina layer	Present	Regionally restricted	1E	Expression was detected in a row of regularly spaced cells located in the outer part of the retina.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Weak		1F	Expression in the ganglion cell layer decreased markedly at P16.
TS28: retina inner nuclear layer	Present	Regionally restricted	1F	Expression was detected in the inner nuclear layer close to the boundary of the inner nuclear layer and outer plexiform layer.

Structure	Level	Pattern	Image	Note
TS19: retina	Present	Regionally restricted		There was almost complete overlap of expression with Math5 in the neuroblast layer of the central retina.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	3A'' , 3A , 3A'''	There was almost complete overlap of expression with Math5 in the neuroblast layer of the central retina. Expression was also detected in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	3B''' , 3B , 3B''	Expression was detected in the ganglion cells layer. In the neuroblast layer, the overlap with Math5 was significantly reduced.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	3C'' , 3C''' , 3C	All retinal ganglion cells in the ganglion cell layer expressed both Pou4f2 and Oc1. In the neuroblast layer Pou4f2-positive cells expressed Oc1, but only a subset of Oc1-positive cells also expressed Pou4f2.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	3D''' , 3D'' , 3D	All retinal ganglion cells in the ganglion cell layer expressed both Pou4f2 and Oc1. Overlap of expression in the neuroblast layer decreased.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted		There was overlap of expression with Isl1 in both the ganglion cell layer and the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted		There was overlap of expression with Isl1 in both the ganglion cell layer and the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS20: retina	Present	Regionally restricted	6B''' , 6B' , 6B	Most expressing cells in the neuroblast layer were also PCNA-positive, but some cells were PCNA-negative.

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	6E , 6E''' , 6E'	At E14.5, many more expressing cells became postmitotic.