Rorbtm1.1(cre)Hze
Targeted Allele Detail
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Symbol: |
Rorbtm1.1(cre)Hze |
Name: |
RAR-related orphan receptor beta; targeted mutation 1.1, Hongkui Zeng |
MGI ID: |
MGI:5507855 |
Synonyms: |
Rorb-IRES2-Cre, Rorb-IRES2-Cre-Delta, Rorb-IRES2-Cre-DeltaNeo/Hygro, Rorb-IRES2-Cre Rorb-IRES2-Cre-DeltaNeo/Hygro, Rorbm1(IRES2-Cre)Hze |
Gene: |
Rorb Location: Chr19:18907969-19088560 bp, - strand Genetic Position: Chr19, 13.28 cM
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Alliance: |
Rorbtm1.1(cre)Hze page
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Allele Type: |
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Targeted (Recombinase) |
Mutation: |
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Insertion
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Rorbtm1.1(cre)Hze expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting vector contained, from 5' to 3', a partial Rorb sequence from intron 9 (with an frt3), a partial Rorb exon 10 sequence including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence, a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. This construct was electroporated into G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replace it with the recombined AttB/AttP site (AttL). The resulting Rorb-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice to remove PhiC31 transgene.
(J:146821, J:220523)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Rorb
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76 |
All: |
21 reference(s) |
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