Gene Expression Data
Assay Details
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Assay
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Reference:
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J:71389
Manley NR, et al., Hoxb2 and hoxb4 act together to specify ventral body wall formation. Dev Biol. 2001 Sep 1;237(1):130-44
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5505792
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Gene symbol:
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Hoxb3
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Gene name:
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homeobox B3
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Probe:
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Hoxb3 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
7A:
embryonic day 12.5; Hoxb4tm1Nrm/Hoxb4 +
(more  )
Specimen
7A:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Hoxb4tm1Nrm/Hoxb4+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: body wall |
Strong |
Regionally restricted |
7A
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Expression was strong in the ventral body wall.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Hoxb4tm1Nrm/Hoxb4tm1Nrm
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: body wall |
Strong |
Regionally restricted |
7B
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Expression was similar to control in the ventral body wall, but the expression domain appears thinner because the body wall is thinner in the mutant.
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Specimen
7C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Hoxb2tm1Mrc/Hoxb2tm1Mrc
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: body wall |
Absent |
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7C
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Expression was lost or strongly reduced in the ventral body wall of the mutant.
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Analysis Tools