Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Regionally restricted	2A	Expressed in migrating melanoblasts.
TS20: pigmented retinal epithelium	Absent		2A

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Regionally restricted	2B	Expressed in migrating melanoblasts.

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Regionally restricted	2C	Expressed in migrating cells just underneath the surface ectoderm in a dorsolateral pathway throughout the embryo.
TS20: pinna mesenchyme	Present	Regionally restricted	2C	Expressed in migrating cells just underneath the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS20: pigmented retinal epithelium	Absent		2D
TS20: skin	Present	Spotted	2D	Only expressed in small clusters of cells in the cranial and sacral region. Migrating melanoblasts were almost completely missing.

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Spotted	2E	Only expressed in small clusters of cells in the cranial and sacral region. Migrating melanoblasts were almost completely missing.

Structure	Level	Pattern	Image	Note
TS20: pinna mesenchyme	Ambiguous		2F	Migrating melanoblasts were almost completely missing, except for small clusters of cells in the cranial and sacral region.
TS20: skin	Ambiguous		2F	Migrating melanoblasts were almost completely missing, except for small clusters of cells in the cranial and sacral region.

Structure	Level	Pattern	Image	Note
TS19: mesenchyme derived from neural crest	Present	Regionally restricted	3A	Expressed in newly emerging melanoblasts that disperse laterally away from the neural tube.

Structure	Level	Pattern	Image	Note
TS19: mesenchyme derived from neural crest	Present	Regionally restricted	3B	Expressed in newly emerging melanoblasts that disperse laterally away from the neural tube.

Structure	Level	Pattern	Image	Note
TS19: mesenchyme derived from neural crest	Present	Regionally restricted	3C	Expressed in migrating melanoblasts, which have reached the base of the hindlimb.

Structure	Level	Pattern	Image	Note
TS19: mesenchyme derived from neural crest	Present	Regionally restricted	3D	Expressed in migrating melanoblasts, which have not reached the base of the hindlimb. Migration stalled at the dorsal end of the dermomyotome.

Structure	Level	Pattern	Image	Note
TS19: mesenchyme derived from neural crest	Present	Regionally restricted	3E	Expressed in migrating melanoblasts, which have not reached the base of the hindlimb. Migration stalled at the dorsal end of the dermomyotome.

Structure	Level	Pattern	Image	Note
TS19: enteric nervous system	Present	Regionally restricted	6A left	The migrating precursors are just rostral to the developing cecum.

Structure	Level	Pattern	Image	Note
TS19: enteric nervous system	Present	Regionally restricted	6A right	Delayed migration in the gastrointestinal tract. The migrating precursors are well rostral to the developing cecum.

Structure	Level	Pattern	Image	Note
TS20: enteric nervous system	Present	Regionally restricted	6B left	Expression was seen from the foregut to the cecum.

Structure	Level	Pattern	Image	Note
TS20: enteric nervous system	Present	Regionally restricted	6B right	Delayed migration in the gastrointestinal tract. The migrating precursors are still rostral to the cecum. Expressed in the mesenteric neuron precursors.

Structure	Level	Pattern	Image	Note
TS20: enteric nervous system	Present	Regionally restricted	6C left	Expression was seen from the foregut to the cecum.

Structure	Level	Pattern	Image	Note
TS20: enteric nervous system	Present	Regionally restricted	6C right	Delayed migration. The migrating precursors are still rostral to the cecum approaching the ileocecal junction. Occationally there are aganglionic regions in the distal small intestine. Expressed in the mesenteric neuron precursors.

Structure	Level	Pattern	Image	Note
TS22: enteric nervous system	Present	Regionally restricted	6D left	Expreesed in the whole gut.

Structure	Level	Pattern	Image	Note
TS22: enteric nervous system	Present	Scattered	6D right	Expressed in the proximal colon but never in the distal colon or the cecum. Expressed in the mesenteric neuron precursors.

Structure	Level	Pattern	Image	Note
TS20: enteric nervous system	Present	Regionally restricted	6E	Expressed in the intestinal tract, not in mesentery.

Structure	Level	Pattern	Image	Note
TS20: enteric nervous system	Present	Regionally restricted	6F	Expressed in the intestinal tract and the mesentery. These cells appear to traverse across the mesentery to populate the proximal colon without populating the cecum.

Structure	Level	Pattern	Image	Note
TS20: enteric nervous system	Present	Regionally restricted	6G	Expressed in the intestinal tract and the mesentery. These cells appear to traverse across the mesentery to populate the proximal colon without populating the cecum.

Structure	Level	Pattern	Image	Note
TS21: enteric nervous system	Present	Regionally restricted	6H	Expressed in the intestinal tract and the mesentery. These cells appear to traverse across the mesentery to populate the proximal colon without populating the cecum.

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from neural crest	Present	Regionally restricted	7A	Expressed in migrating melanoblasts throughout the rostral-caudal axis.
TS15: dorsal root ganglion	Present		7A
TS15: facio-acoustic preganglion complex	Present		7A
TS15: glossopharyngeal-vagus preganglion complex	Present		7A
TS15: trigeminal preganglion	Present		7A
TS15: peripheral nervous system	Present	Regionally restricted	7A	Expressed in the sympathetic ganglia chain and in enteric neuron precursors.

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		7B
TS15: glossopharyngeal-vagus preganglion complex	Present		7B
TS15: trigeminal preganglion	Present		7B

Structure	Level	Pattern	Image	Note
TS15: peripheral nervous system	Present	Regionally restricted	7C	Expressed in enteric neuron precursors populating the foregut mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present		7D left
TS17: enteric nervous system	Present		7D left
TS17: facial ganglion	Present		7D left
TS17: glossopharyngeal ganglion	Present		7D left
TS17: neural tube	Present		7D left
TS17: sympathetic nervous system	Present		7D left
TS17: trigeminal ganglion	Present		7D left
TS17: vagus ganglion	Present		7D left
TS17: peripheral nervous system	Present	Regionally restricted	7D left	Also expressed in peripheral nerves the intercostals and the nerves in the limbs.
TS17: heart	Strong		7D left

Structure	Level	Pattern	Image	Note
TS17: heart	Strong		7D right
TS17: acoustic ganglion	Present		7D right
TS17: enteric nervous system	Present		7D right
TS17: facial ganglion	Present		7D right
TS17: glossopharyngeal ganglion	Present		7D right
TS17: neural tube	Present		7D right
TS17: sympathetic nervous system	Present		7D right
TS17: trigeminal ganglion	Present		7D right
TS17: vagus ganglion	Present		7D right
TS17: peripheral nervous system	Present	Regionally restricted	7D right	Also expressed in peripheral nerves the intercostals and the nerves in the limbs.

Structure	Level	Pattern	Image	Note
TS21: eye	Present		7E left
TS21: forebrain	Present		7E left
TS21: midbrain	Present		7E left
TS21: spinal cord	Present		7E left
TS21: dorsal root ganglion	Present		7E left
TS21: enteric nervous system	Present		7E left
TS21: facial ganglion	Present		7E left
TS21: glossopharyngeal ganglion	Present		7E left
TS21: sympathetic ganglion	Present		7E left
TS21: trigeminal ganglion	Present		7E left
TS21: vagus ganglion	Present		7E left
TS21: vestibulo-cochlear ganglion	Present		7E left
TS21: peripheral nervous system	Present	Regionally restricted	7E left	Also expressed in peripheral nerves the intercostals and the nerves in the limbs.

Structure	Level	Pattern	Image	Note
TS21: eye	Present		7E right
TS21: forebrain	Present		7E right
TS21: midbrain	Present		7E right
TS21: spinal cord	Present		7E right
TS21: dorsal root ganglion	Present		7E right
TS21: enteric nervous system	Present		7E right
TS21: facial ganglion	Present		7E right
TS21: glossopharyngeal ganglion	Present		7E right
TS21: sympathetic ganglion	Present		7E right
TS21: trigeminal ganglion	Present		7E right
TS21: vagus ganglion	Present		7E right
TS21: vestibulo-cochlear ganglion	Present		7E right
TS21: peripheral nervous system	Present	Regionally restricted	7E right	Also expressed in peripheral nerves the intercostals and the nerves in the limbs.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		8A
TS19: heart atrium	Present	Regionally restricted	8A	Expressed in the walls.
TS19: heart ventricle	Present	Regionally restricted	8A	Expressed in the walls.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		8B
TS19: neural tube	Present	Regionally restricted	8B	In the rostral neural tube, expression is throughout the dorsal part and in a small area of the ventral.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		8C
TS19: neural tube	Strong	Regionally restricted	8C	Strong expression in the caudal neural tube, except for the most dorsal aspect, the ventral floorplate and the region around the differentiating motor neurons.

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present		8D
TS19: facial ganglion	Present		8D
TS19: glossopharyngeal ganglion	Present		8D
TS19: neural tube	Present		8D
TS19: trigeminal ganglion	Present		8D
TS19: vagus ganglion	Present		8D
TS19: diencephalon	Present	Regionally restricted	8D	Expressed in the walls.
TS19: myelencephalon	Present	Regionally restricted	8D	Expressed in the walls.
TS19: telencephalon	Present	Regionally restricted	8D	Expressed in the walls.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme derived from neural crest	Present	Scattered		Expressed in scattered cells on the surface of the head.
TS17: enteric nervous system	Present

Structure	Level	Pattern	Image	Note
TS17: enteric nervous system	Present			Migration patter is the same as the heterozygous.

Structure	Level	Pattern	Image	Note
TS15: peripheral nervous system	Present	Regionally restricted		Expressed in the enteric neuron precursors.

Structure	Level	Pattern	Image	Note
TS15: peripheral nervous system	Present	Regionally restricted		Expressed in the enteric neuron precursors. Migration patter is the same as the heterozygous.

Structure	Level	Pattern	Image	Note
TS17: enteric nervous system	Present	Regionally restricted		Expressed from the foregut to the most distal portion of the hindgut

Structure	Level	Pattern	Image	Note
TS19: liver	Present
TS19: lung	Present