Probe:	IMAGE clone 2922158
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2A	Expression localized to preplate. No expression in ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present	Regionally restricted	2B	Expression in cortical plate across entire periphery, terminating sharply at the piriform cortex.
TS21: ganglionic eminence	Trace		2B	Very weak expression in subpallial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		2C
TS21: thalamus	Strong	Regionally restricted	2C	Strong expression in thalamic eminences (transient structures at base of dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong		2D
TS23: cerebral cortex intermediate zone	Weak		2D
TS23: ganglionic eminence	Weak		2D

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong		2E
TS23: cerebral cortex intermediate zone	Weak		2E
TS23: ganglionic eminence	Present		2E

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong		2F
TS23: cerebral cortex intermediate zone	Weak		2F
TS23: ganglionic eminence	Weak		2F
TS23: thalamus	Weak		2F
TS23: telencephalon	Present	Regionally restricted	2F	Expression in medial limbic cortex extending to cortical hem.
TS23: diencephalon	Weak	Regionally restricted	2F	Light expression in walls of neural tube enclosing third ventricle and fourth ventricle.
TS23: hindbrain	Weak	Regionally restricted	2F	Light expression in walls of neural tube enclosing third ventricle and fourth ventricle.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Present		2G	Expression similar to that observed at E15.5, except for clearer definiition of expression in thicker cortical plate.
TS25: cerebral cortex subventricular zone	Strong	Regionally restricted	2G	Increased expression in subventricular zone overlying the ventricular zone in both neocortex and basal ganglia.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Strong		2H
TS25: cortical subplate	Strong		2H
TS25: cerebral cortex intermediate zone	Weak		2H
TS25: cerebral cortex subventricular zone	Weak		2H
TS25: cerebral cortex ventricular zone	Absent		2H

Structure	Level	Pattern	Image	Note
TS27: neocortex	Weak	Regionally restricted	2I	Faint expression in deep layers of neocortex.
TS27: cingulate cortex	Present		2I
TS27: hippocampus CA1	Present		2I
TS27: dentate gyrus	Trace		2I
TS27: hippocampus CA3	Trace		2I

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		2J
TS27: cerebellum internal granule cell layer	Present		2J

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present		2K
TS28: neocortex	Absent		2K