Probe:	mdc8-3
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	5A	Expression was detected in the dorsal telencephalon.
TS19: retina	Present		5A
TS19: head mesenchyme	Present	Regionally restricted	5A	Expression was detected in the mesenchyme surrounding the eye.
TS19: trigeminal ganglion	Present		5A
TS19: cranial ganglion	Present	Regionally restricted	5A	Expression was detected in the trigeminal ganglia and the vestibular ganglia.
TS19: inner ear	Present	Regionally restricted	5A	Expression was detected in the otic vesicle.
TS19: metencephalon basal plate	Present	Regionally restricted	5A	Expression was detected in the region of the future pons.
TS19: hindbrain ventricular layer	Present		5A

Structure	Level	Pattern	Image	Note
TS23: retina	Present		5C	Expression was detected throughout the retina.

Structure	Level	Pattern	Image	Note
TS25: retina inner nuclear layer	Present		5E
TS25: retina	Present	Regionally restricted	5E	Expression was detected in the ventricular layer.
TS25: retina outer nuclear layer	Absent		5E

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present		5G
TS27: retina	Present	Regionally restricted	5G	Expression was detected in the ventricular layer.
TS27: retina outer nuclear layer	Absent		5G

Structure	Level	Pattern	Image	Note
TS15: eye	Present		6A
TS15: otocyst	Present		6A
TS15: telencephalon	Present		6A

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		6B	Expression was detected as in wild type.
TS15: telencephalon	Present		6B	Expression was detected as in wild type.
TS15: eye	Present		6B	Expression appeared more intense than in wild type, but this was found to be an artifact.

Structure	Level	Pattern	Image	Note
TS15: optic cup	Present	Regionally restricted	6C	Expression was detected in the retina.
TS15: eye mesenchyme	Present	Regionally restricted	6C	Expression was detected in the mesenchyme surrounding the eye.

Structure	Level	Pattern	Image	Note
TS15: optic cup	Present	Regionally restricted	6D	Expression was detected in the retina as in wild type.
TS15: eye mesenchyme	Present	Regionally restricted	6D	Expression was detected in the mesenchyme surrounding the eye as in wild type.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present

Structure	Level	Pattern	Image	Note
TS20: inner ear	Present	Regionally restricted	7B	Expression was detected in the medial wall of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS20: inner ear	Present	Regionally restricted	7E	Expression was detected in the medial wall of the otic vesicle at similar levels to wild type.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		9A

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		9B	Expression was maintained in the mutant.