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Gene Expression Data
Assay Details
Assay
Reference: J:76260 Chen JC, et al., Essential and redundant functions of the MyoD distal regulatory region revealed by targeted mutagenesis. Dev Biol. 2002 May 1;245(1):213-23
Assay type: Northern blot
MGI Accession ID: MGI:5501481
Gene symbol: Myod1
Gene name: myogenic differentiation 1
Probe: Myod1 cDNA6
Probe preparation: Double stranded labelled with P32 DNA
Visualized with: Phosphorimaging
Assay notes: Quantification of expression is shown in Figure 5B.
Results
Image: 5 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
+/+ P7 TS28: forelimb musculature
TS28: hindlimb musculature 		Present 5-10 µg Not Specified Not Specified
+/loxP P7 TS28: forelimb musculature
TS28: hindlimb musculature 		Present 5-10 µg involves: 129 * C57BL/6J Myod1tm2.1Glh/Myod1+Not Specified
loxP/loxP P7 TS28: forelimb musculature
TS28: hindlimb musculature 		Present (a) 5-10 µg involves: 129 * C57BL/6J Myod1tm2.1Glh/Myod1tm2.1GlhNot Specified
+/+ P w 12 TS28: tibialis anterior Present 5-10 µg Not Specified Not Specified
loxP/loxP P w 12 TS28: tibialis anterior Present (b) 5-10 µg involves: 129 * C57BL/6J Myod1tm2.1Glh/Myod1tm2.1GlhNot Specified
Notes:
(a) Expression in this mutant decreased about 15% compared to wild-type embryos.
(b) There was an 65% reduction in expression in this mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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