Probe:	SorLA probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	For some specimens, SorLA probe3 (MGI:5498361) may have been used. Authors state that both probes yielded the same hybridization pattern.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Regionally restricted	2C/D	Highest expression detected over neurons in the cerebral cortex.
TS28: dentate gyrus	Strong	Regionally restricted	2C/D	Intense expression over granular cells in dentate gyrus of hippocampus.
TS28: cerebellum	Present		2C/D

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Strong		2E	Highest expression in layer 5, containing pyramidal cells.

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Strong		2F	Intense expression over pyramidal cells of CA regions.

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Regionally restricted	3A/B	Expression first detected in the area of the postaminiotic fold.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Widespread	3C/D
TS11: amnion	Strong		3C/D
TS11: ectoplacental cone	Absent		3C/D

Structure	Level	Pattern	Image	Note
TS13: amnion	Strong		3E/F
TS13: foregut diverticulum	Strong		3E/F
TS13: future spinal cord neural plate	Absent		3E/F

Structure	Level	Pattern	Image	Note
TS15: foregut	Strong		3H/I
TS15: head mesenchyme	Strong		3H/I
TS15: hindgut	Strong		3H/I
TS15: somite	Strong		3H/I

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Strong	Regionally restricted	3K/L	Strong expression in spinal cord motor neurons.
TS17: dorsal root ganglion	Absent		3K/L
TS17: somite	Strong		3K/L

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Strong	Regionally restricted	3M/N	Strong expression over ventral part of neural tube, extending throughout hindbrain.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Strong	Regionally restricted	3O/P	Strong expression in spinal cord motor neurons.
TS17: dorsal root ganglion	Absent		3O/P
TS17: embryo mesenchyme	Strong		3O/P

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4A E11.5	Expression restricted to lateral aspects of cerebral cortex, decreasing dorso-laterally.
TS19: myelencephalon	Strong		4A E11.5

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		4A E12.5
TS20: medulla oblongata	Strong		4A E12.5

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		4A E14.5
TS22: medulla oblongata	Weak		4A E14.5
TS22: metencephalon	Weak		4A E14.5

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present		4A E16.5	Slightly lower expression over the entire cortical area.
TS24: nasal cavity epithelium	Present	Regionally restricted	4A E16.5	Expression in serous glands of nasal cavity.
TS24: submandibular gland	Present		4A E16.5

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Weak		4A E18.5	Overall expression in cortex is further decreased.
TS26: nasal cavity epithelium	Present	Regionally restricted	4A E18.5	Expression in serous glands of nasal cavity.
TS26: pancreas	Present		4A E18.5
TS26: submandibular gland	Present		4A E18.5

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	4A P0	Stronger expression in outer aspects of cortical plate than in subventricular zone.
TS27: submandibular gland	Present		4A P0

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	4C/D	Strong expression in outer layer of neuronal tissue of cerebral cortex, where neurons have just left the ventricular zone.
TS19: cerebellum primordium	Present	Regionally restricted	4C/D	Expression in a thin cell layer in rhombic lip of metencephalon.
TS19: myelencephalon	Strong		4C/D

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Weak
TS20: midbrain	Weak

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	4G/H	Expression becomes more even and spreads to more dorsal and caudal locations.
TS22: diencephalon	Weak		4G/H	Decreased expression in metencephalon and myelencephalon.
TS22: midbrain	Weak		4G/H	Decreased expression in metencephalon and myelencephalon.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	4I/J	Expression in telencephalon becomes more even and spreads to more dorsal and caudal locations.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Strong		5A/B
TS24: cortical plate	Strong	Regionally restricted	5A/B	Strong expression in outer aspects of cortical plate.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Strong		5C/D
TS24: cortical plate	Strong	Regionally restricted	5C/D	Strong expression in outer aspects of cortical plate.

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Weak	Regionally restricted	5E/F	Weak expression over mitral layer of olfactory bulb.
TS24: hypothalamus	Trace		5E/F	Expression is just above background.
TS24: pineal gland	Present		5E/F
TS24: choroid plexus	Absent		5E/F

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	5G/H	Weaker expression in outer aspects of cortical plate than in subventricular zone.
TS26: pituitary gland	Present		5G/H
TS26: nasal cavity epithelium	Present	Regionally restricted	5G/H	Expression in serous glands of the nasal cavity.
TS26: choroid plexus	Absent		5G/H

Structure	Level	Pattern	Image	Note
TS26: retina	Present
TS26: thalamus	Present	Regionally restricted		Expression in the geniculate nucleus of thalamus.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	5I/J	Stronger expression in outer aspects of cortical plate than in subventricular zone.
TS27: geniculate thalamic group	Present		5I/J	Expression in the geniculate nucleus of thalamus.
TS27: pituitary gland	Present		5I/J
TS27: choroid plexus	Absent

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	5K/L	Stronger expression in outer aspects of cortical plate than in subventricular zone.
TS27: corpus striatum	Absent		5K/L

Structure	Level	Pattern	Image	Note
TS27: retina	Present

Structure	Level	Pattern	Image	Note
TS24: trigeminal ganglion	Present		6A/B

Structure	Level	Pattern	Image	Note
TS24: sympathetic ganglion	Present
TS24: vestibulo-cochlear ganglion	Present
TS24: liver	Trace	Spotted
TS24: spleen	Trace	Spotted
TS24: thymus	Trace	Spotted

Structure	Level	Pattern	Image	Note
TS26: dorsal root ganglion	Present		6C/D

Structure	Level	Pattern	Image	Note
TS20: pituitary gland	Present		7A/B

Structure	Level	Pattern	Image	Note
TS27: pituitary gland	Present		7C/D

Structure	Level	Pattern	Image	Note
TS24: pineal gland	Present		7E/F

Structure	Level	Pattern	Image	Note
TS26: thyroid gland	Present		7G/H
TS26: aorta	Absent		7G/H	No expression in endothelia lining the aorta.

Structure	Level	Pattern	Image	Note
TS27: Harderian gland	Present		7I/J

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Present		8A/B

Structure	Level	Pattern	Image	Note
TS19: stomach epithelium	Present		8C/D

Structure	Level	Pattern	Image	Note
TS20: lung	Present		8E/F

Structure	Level	Pattern	Image	Note
TS22: lung	Present		8G/H

Structure	Level	Pattern	Image	Note
TS19: metanephros	Present	Regionally restricted	8I/J	Expression in tubules of kidney.

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Present		8K/L

Structure	Level	Pattern	Image	Note
TS24: gut	Present	Regionally restricted	8M/N	Expression between the two outer walls of the gut, where the enteric ganglia reside.

Structure	Level	Pattern	Image	Note
TS26: gut	Present	Regionally restricted	8O/P	Expression between the two outer walls of the gut, where the enteric ganglia reside.
TS26: aorta	Absent		8O/P	No expression in endothelia lining the aorta.