Gene Expression Data
Assay Details
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Assay
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Reference:
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J:36988
Zappelli F, et al., The inhibition of differentiation caused by TGFbeta in fetal myoblasts is dependent upon selective expression of PKCtheta: a possible molecular basis for myoblast diversification during limb histogenesis. Dev Biol. 1996 Nov 25;180(1):156-64
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5490103
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Gene symbol:
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Prkcq
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Gene name:
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protein kinase C, theta
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Antibody:
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Anti-PKC theta
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to rhodamine
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Results
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Specimen
3C:
embryonic day 13.0
(more  )
Specimen
3C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: limb |
Weak |
Non-Uniform |
3C
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The great majority of cells showed no immunoreactivity. Only a few clusters of cells showed some immunoreactivity.
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Specimen
3D:
embryonic day 17.0
(more )
Specimen
3D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: limb musculature |
Present |
Non-Uniform |
3D
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Sarcoplasmic expression was detected in most muscle fibers, with smaller secondary fibers showing stronger staining.
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Analysis Tools
