Gene Expression Data
Assay Details
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Assay
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Reference:
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J:51099
Asakura A, et al., Apoptosis of epaxial myotome in Danforth's short-tail (Sd) mice in somites that form following notochord degeneration. Dev Biol. 1998 Nov 15;203(2):276-89
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5489065
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Gene symbol:
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Pax1
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Gene name:
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paired box 1
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Probe:
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Pax1 probe19
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Embryos were fixed in glutaraldehyde/paraformaldehyde (0.2%/0.4%).
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Results
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Specimen
3A:
embryonic day 10.0
(more  )
Note: Also stained for Shh (assay MGI:5489063).
Specimen
3A:
(close  )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Also stained for Shh (assay MGI:5489063).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: sclerotome |
Present |
Widespread |
3A
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Expression in entire sclerotome from cervical to tailbud region.
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Specimen
3B:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Also stained for Shh (assay MGI:5489063).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: sclerotome |
Present |
Regionally restricted |
3B
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Expression in entire sclerotome of rostral region; no expression in sclerotomal region in caudal somites.
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Specimen
3C:
embryonic day 10.0
(more )
Note: Also stained for Shh (assay MGI:5489063).
Specimen
3C:
(close )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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Note:
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Also stained for Shh (assay MGI:5489063).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: sclerotome |
Present |
Widespread |
3C
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Expression in entire sclerotome from cervical to tailbud region.
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Specimen
3D:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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Note:
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Also stained for Shh (assay MGI:5489063).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: sclerotome |
Present |
Regionally restricted |
3D
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Expression in entire sclerotome of rostral region; no expression in sclerotomal region in caudal somites.
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Analysis Tools