Probe:	Alx3 cDNA1
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted		Expression is present in a region near the tail bud.
TS12: allantois	Present

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	4A	Expression is present in a region near the tail bud.
TS13: allantois	Present		4A

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Regionally restricted	4B	Expression is present in a region near the tail bud.

Structure	Level	Pattern	Image	Note
TS14: head	Present	Regionally restricted	4C	Expression is present in craniofacial structures; it is particularly high in the lower frontal part of the head, with a rostral border near the level of the eye.
TS14: head mesenchyme derived from neural crest	Strong		4C	Expression is present in the anterior mesenchyme.
TS14: trunk mesenchyme	Strong	Regionally restricted	4C	Expression is present at multiple paired areas in the flank mesoderm and in the body wall; the anterior areas overlap the areas where the limbs develop.

Structure	Level	Pattern	Image	Note
TS14: surface ectoderm	Present	Regionally restricted	4D	Expression is high in the ventral surface ectoderm between the allantois and tail bud.
TS14: allantois	Present		4D
TS14: amnion	Present		4D

Structure	Level	Pattern	Image	Note
TS15: head	Present	Regionally restricted	4E	Expression is present in craniofacial structures; it is particularly high in the lower frontal part of the head, with a rostral border near the level of the eye.
TS15: head mesenchyme derived from neural crest	Strong		4E	Expression is present in the anterior mesenchyme.
TS15: 1st branchial arch	Present	Regionally restricted	4E	Expression is located distally.
TS15: trunk mesenchyme	Strong	Regionally restricted	4E	Expression is present at multiple paired areas in the flank mesoderm and in the body wall. This includes the areas that will become the genital eminence and the limbs.
TS15: forelimb bud	Strong	Non-Uniform	4E	Expression is higher in the anterior part of the limb bud.

Structure	Level	Pattern	Image	Note
TS15: embryo ectoderm	Present	Regionally restricted	4F	There is expression in the ectomesenchyme that flanks the diencephalon.
TS15: head mesenchyme	Present	Regionally restricted	4F	There is expression in the ectomesenchyme that flanks the diencephalon.
TS15: diencephalon	Absent		4F

Structure	Level	Pattern	Image	Note
TS17: head	Present	Regionally restricted	4G	Expression is present in craniofacial structures; it is particularly high in the lower frontal part of the head, with a rostral border near the level of the eye.
TS17: 1st branchial arch	Present	Regionally restricted	4G	Expression is located distally.
TS17: 2nd branchial arch	Present	Regionally restricted	4G	Expression is located distally.
TS17: fronto-nasal process	Present		4G
TS17: head mesenchyme derived from neural crest	Present		4G
TS17: hindlimb bud	Present	Regionally restricted	4G	The expression domain extends to distal and distal-posterior regions.
TS17: trunk mesenchyme	Present		4G	Expression is present in lateral plate mesoderm.

Structure	Level	Pattern	Image	Note
TS19: head	Present	Regionally restricted	4I	Expression is present in craniofacial structures; it is particularly high in the lower frontal part of the head, with a rostral border near the level of the eye.
TS19: trunk mesenchyme	Present	Regionally restricted	4I	Expression is present in a bipartite domain consisting of a larger anterior and smaller distal-posterior domain.
TS19: genital tubercle	Present	Regionally restricted	4I	Expression is present in the caudal part. This is shown at higher magnification in the inset.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present	Regionally restricted	8B	Expression is present distally.