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Gene Expression Data
Assay Details
Assay
Reference: J:176654 McCormick JA, et al., A SPAK Isoform Switch Modulates Renal Salt Transport and Blood Pressure. Cell Metab. 2011 Sep 7;14(3):352-64
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5473386
Gene symbol: Slc12a3
Gene name: solute carrier family 12, member 3
Antibody: pT53-NCC antibody
Detection system: Secondary antibody
Assay notes: This antibody recognizes the phosphorylated isoform (Thr53).
Results
Specimen 5A WT, merge: postnatal (more )
Note: Double labeled: red - Slc12a3 (pT53-NCC; this assay); green - Slc12a3 (total protein; assay MGI:5472011).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 5A Wild Type pT53 NCC , 5A Wild Type Merge
Specimen 5A KO, merge: postnatal; Stk39tm1Dlp/Stk39tm1Dlp (more )
Note: Double labeled: red - Slc12a3 (pT53-NCC; this assay); green - Slc12a3 (total protein; assay MGI:5472011).
Structure Level Pattern Image Note
TS28: distal convoluted tubule Present 5A Knockout Merge , 5A Knockout pT53 NCC
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory