Probe:	collagenase I probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS22: mandible	Present		1a	Expression first detected at E14.5.
TS22: maxilla	Present		1a	Expression first detected at E14.5.
TS22: palatal shelf	Present		1a	Expression first detected at E14.5.
TS22: femur cartilage condensation	Present	Regionally restricted	1a	Expression in ossification center (shaft region).

Structure	Level	Pattern	Image	Note
TS22: basioccipital cartilage condensation	Present	Regionally restricted	1b/c	Expression in ossification center (shaft region).

Structure	Level	Pattern	Image	Note
TS22: basioccipital cartilage condensation	Present	Single cells	1d

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Present	Regionally restricted		Expression in ossification center (shaft region).

Structure	Level	Pattern	Image	Note
TS24: clavicle	Present		2a/b
TS24: mandible	Present		2a/b
TS24: hip	Present	Regionally restricted	2a/b	Authors report expression in the hip bone.
TS24: heart	Absent		2a/b
TS24: metanephros	Absent		2a/b
TS24: skeletal muscle	Absent		2a/b
TS24: skin	Absent		2a/b
TS24: rib	Present	Regionally restricted	2a/b	Expression in dorsal, but not in ventral, parts of ribs.

Structure	Level	Pattern	Image	Note
TS24: mandible	Strong	Regionally restricted	2d	Expression in basophilic cells along newly formed bone in the mandible, presumably representing osteoblasts.

Structure	Level	Pattern	Image	Note
TS24: mandible	Strong	Regionally restricted	2e	High expression in cells lining the bone matrix.

Structure	Level	Pattern	Image	Note
TS24: radius	Strong	Regionally restricted	3a/b	High levels of expression in diaphysis of radius and ulna. Expression confined to cells localized along bone trabeculae and in the zone of hypertrophic chondrocytes.
TS24: ulna	Strong	Regionally restricted	3a/b	High levels of expression in diaphysis of radius and ulna. Expression confined to cells localized along bone trabeculae and in the zone of hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: ulna	Present	Regionally restricted	3d/e	Expression in cells of calcified cartilage and in area of primary spongiosa. No expression in upper zone of hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present
TS24: scapula	Present
TS24: vertebra	Present
TS24: chondrocranium	Present	Regionally restricted		Expression in the base of the skull (exoccipital).
TS24: exoccipital bone	Present			Expression in the base of the skull (exoccipital).

Structure	Level	Pattern	Image	Note
TS24: skeleton	Present	Regionally restricted	5a	Expression in ossifying tissues of embryo.
TS24: clavicle	Present	Regionally restricted	5a	Expression in central region of clavicle, scapula and dorsal part of rib.
TS24: rib	Present	Regionally restricted	5a	Expression in central region of clavicle, scapula and dorsal part of rib.
TS24: scapula	Present	Regionally restricted	5a	Expression in central region of clavicle, scapula and dorsal part of rib.

Structure	Level	Pattern	Image	Note
TS28: femur	Present	Regionally restricted	6a/b	Expression in the epiphysis and metaphysis of the distal femur.
TS28: long bone epiphysis	Present		6a/b	Expression in the epiphysis and metaphysis of the distal femur.
TS28: long bone metaphysis	Present		6a/b	Expression in the epiphysis and metaphysis of the distal femur.
TS28: epiphyseal plate hypertrophic zone	Strong	Regionally restricted	6a/b	High level of expression in cells in the lower zone of hypertrophic chondrocytes in calcified cartilage.

Structure	Level	Pattern	Image	Note
TS28: long bone	Strong	Regionally restricted	7a	High expression in basophilic cells localiized along newly formed bone trabeculae.

Structure	Level	Pattern	Image	Note
TS28: long bone	Present	Regionally restricted	7b	Preferential expression in basophilic cells on osteoid surfaces adjacent to multinuclear TRAP-positive cells.

Structure	Level	Pattern	Image	Note
TS28: long bone diaphysis	Present	Regionally restricted	7c	Distinct expression in osteoblasts in the diaphyseal periosteum and in a few osteocytes of the peripheral area of cortex.