Probe:	UI-M-BH3-arf-b-08-0-UI.r1
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Absent

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Non-Uniform	2B	Expression was detected in the brain with the highest levels in the marginal areas.
TS19: olfactory epithelium	Absent		2B

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Non-Uniform	2H	Expression was detected in the brain and spinal cord with the highest levels in the marginal areas.
TS19: future spinal cord	Present	Non-Uniform	2H	Expression was detected in the brain and spinal cord with the highest levels in the marginal areas.
TS19: cranial ganglion	Present		2H
TS19: dorsal root ganglion	Present		2H
TS19: inner ear	Absent		2H

Structure	Level	Pattern	Image	Note
TS19: future pituitary	Weak
TS19: head	Present	Regionally restricted		Expression was virtually absent from orofacial epithelia and mesenchyme outside of the tooth domains.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present		2L
TS20: cranial ganglion	Present		2L
TS20: inner ear	Absent		2L
TS20: pituitary gland	Weak		2L

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present
TS20: lens vesicle epithelium	Present
TS20: retina	Present	Regionally restricted		Expression was detected in the retinal ganglionic cell layer.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Widespread	3B/B'	Expression was detected throughout the neuraxis, particularly in differentiating fields.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Widespread	3G/G'	Expression was detected throughout the neuraxis, particularly in differentiating fields.

Structure	Level	Pattern	Image	Note
TS21: brain	Present	Regionally restricted	4B	Expression was prominent in the neuraxis. The highest levels were detected in the subventricular zone and differentiating fields.

Structure	Level	Pattern	Image	Note
TS21: brain	Present	Regionally restricted	4G	Expression was prominent in the neuraxis. The highest levels were detected in the subventricular zone and differentiating fields.

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Regionally restricted	4L	Expression was prominent in the neuraxis. The highest levels were detected in the subventricular zone and differentiating fields.
TS22: trigeminal ganglion	Present		4L
TS22: pituitary gland	Weak		4L
TS22: cochlea	Trace		4L

Structure	Level	Pattern	Image	Note
TS22: retina	Present	Regionally restricted	4Q	Expression was highest in the retinal ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS26: brain	Strong		4T
TS26: retina	Present	Regionally restricted	4T	Expression was increased in the retinal ganglion cells.

Structure	Level	Pattern	Image	Note
TS26: inner ear epithelium	Absent		4V

Structure	Level	Pattern	Image	Note
TS26: cranial ganglion	Strong
TS26: main olfactory bulb	Strong
TS26: epidermis	Present	Regionally restricted		Expression was detected in the suprabasal layers of the epidermis.

Structure	Level	Pattern	Image	Note
TS19: jaw primordium mesenchyme	Present	Regionally restricted	5C , 5D	Expression was detected in the dental mesenchyme.
TS19: dental placode	Present		5C , 5D

Structure	Level	Pattern	Image	Note
TS21: incisor mesenchyme	Present	Regionally restricted	5E	Expression was detected in the dental papilla mesenchyme of incisors.

Structure	Level	Pattern	Image	Note
TS22: enamel knot	Present		5G
TS22: molar mesenchyme	Present	Regionally restricted	5G	Expression was detected in the mesenchyme on the vestibular side of the tooth.
TS22: molar dental papilla	Present		5G

Structure	Level	Pattern	Image	Note
TS23: tooth mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS26: incisor	Present	Regionally restricted	5J	Expression was more abundant in preodontoblasts and differentiating odontoblasts than in differentiated odontoblasts. Differentiating and secretory ameloblasts were devoid of labeling.
TS26: facial bone	Present	Regionally restricted	5J	Expression was detected in regions of membrane and endochondral bone formation.

Structure	Level	Pattern	Image	Note
TS26: dental papilla	Present		5N
TS26: facial bone	Present	Regionally restricted	5N	Expression was detected in regions of membrane and endochondral bone formation.

Structure	Level	Pattern	Image	Note
TS28: incisor	Present	Regionally restricted	5S	Expression was detected in cementoblasts and preodontoblasts. Odontoblasts in both crown and roots contained lower transcript levels than preodontoblasts. Secretory and mature ameloblasts were devoid of expression.
TS28: viscerocranium	Present	Regionally restricted	5S	Expression was detected in regions of membrane and endochondral bone formation.

Structure	Level	Pattern	Image	Note
TS28: molar	Present	Regionally restricted	5W , 5X	Expression was detected in cementoblasts and preodontoblasts. Odontoblasts in both crown and roots contained lower transcript levels than preodontoblasts. Secretory and mature ameloblasts were devoid of expression.
TS28: viscerocranium	Present	Regionally restricted	5X , 5W	Expression was detected in regions of membrane and endochondral bone formation.

Structure	Level	Pattern	Image	Note
TS20: jaw primordium mesenchyme	Present	Regionally restricted		Robust expression was detected in the distal/vestibular mesenchyme of the developing upper and lower incisors.

Structure	Level	Pattern	Image	Note
TS20: jaw primordium mesenchyme	Moderate	Regionally restricted	6G	Expression was detected in the molar buds.
TS20: palatal shelf epithelium	Moderate	Regionally restricted	6G	Expression was detected in the epithelium and mesenchyme in the anterior and middle parts of the developing palate, but not the posterior part.
TS20: palatal shelf mesenchyme	Moderate	Regionally restricted	6G	Expression was detected in the epithelium and mesenchyme in the anterior and middle parts of the developing palate, but not the posterior part.

Structure	Level	Pattern	Image	Note
TS21: molar mesenchyme	Present	Regionally restricted		Expression was detected in a cluster of mesenchymal cells on the vestibular side of the molar tooth buds.
TS21: palatal shelf epithelium	Trace	Regionally restricted		Expression was detected at slightly above background in the anterior palatal epithelium and mesenchyme but was virtually absent from the posterior region.
TS21: palatal shelf mesenchyme	Trace	Regionally restricted		Expression was detected at slightly above background in the anterior palatal epithelium and mesenchyme but was virtually absent from the posterior region.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf epithelium	Ambiguous		6L	Expression in the middle region was virtually absent.
TS21: palatal shelf mesenchyme	Ambiguous		6L	Expression in the middle region was virtually absent.
TS21: tongue mesenchyme	Strong	Regionally restricted	6L	Expression was detected in clusters of mesenchymal cells underlying the epithelium on the lateral lingual surface.
TS21: tongue	Present	Regionally restricted	6L	Expression was detected in clusters of laterally located cells flanking the genioglossus muscles.
TS21: facial bone primordium	Present	Regionally restricted	6L	Expression was detected in prehypertrophic chondrocytes and osteoblasts.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Absent		6R
TS22: palatal shelf epithelium	Absent		6R
TS22: palatal shelf mesenchyme	Absent		6R
TS22: tongue mesenchyme	Present	Regionally restricted	6R	Expression was detected in clusters of mesenchymal cells that flank the genioglossus muscles and in cell aggregates located at the baso-lateral margins of the mesenchyme of the posterior tongue.
TS22: facial bone primordium	Present	Regionally restricted	6R	Expression was detected in osteoblasts and prehypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Absent		6U
TS22: palatal shelf epithelium	Absent		6U
TS22: palatal shelf mesenchyme	Absent		6U
TS22: tongue mesenchyme	Present	Regionally restricted	6U	Expression was detected in clusters of mesenchymal cells that flank the genioglossus muscles and in cell aggregates located at the baso-lateral margins of the mesenchyme of the posterior tongue.
TS22: facial bone primordium	Present	Regionally restricted	6U	Expression was detected in osteoblasts and prehypertrophic chondrocytes.
TS22: autonomic ganglion	Present	Regionally restricted	6U	Expression was prominent in the submandibular ganglion.

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Absent		6Y
TS23: palatal shelf epithelium	Absent		6Y
TS23: palatal shelf mesenchyme	Absent		6Y
TS23: tongue mesenchyme	Present	Regionally restricted	6Y , 6Y'	Expression was detected in clusters of mesenchymal cells that flank the genioglossus muscles and in cell aggregates located at the baso-lateral margins of the mesenchyme of the posterior tongue.
TS23: facial bone primordium	Present	Regionally restricted	6Y	Expression was detected in osteoblasts and prehypertrophic chondrocytes.
TS23: autonomic ganglion	Present	Regionally restricted	6Y	Expression was prominent in the submandibular ganglion.

Structure	Level	Pattern	Image	Note
TS28: viscerocranium	Present	Regionally restricted	6Z''	Expression was detected in regions of membrane and endochondral bone formation.