Probe:	pGEM-Lhx9
Probe preparation:	Antisense labelled with radioactivity RNA
Visualized with:	Autoradiography
Assay notes:	Fixative was in 0.1 M 4-morpholinepropanesulfonic acid, pH 7.4, 2 mM EGTA, 1 mM MgSO4 , and 3.7% formaldehyde.

Structure	Level	Pattern	Image	Note
TS17: nervous system	Present

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Widespread	2A	Expression is in the alar neuroepithelium.
TS19: forebrain	Present	Regionally restricted	2A	Expression is in the future cerebral cortex, the hippocampus, the claustrum primordium, and the caudal ganglionic eminence.
TS19: midbrain	Present	Regionally restricted	2A	Expression is in the tectum.
TS19: hindbrain	Present		2A

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Widespread	2B	Expression is in the alar neuroepithelium.
TS19: forebrain	Present	Regionally restricted	2B	Expression is in the future cerebral cortex, the hippocampus, the claustrum primordium, and the caudal ganglionic eminence.
TS19: midbrain	Present	Regionally restricted	2B	Expression is in the tectum.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Widespread	2C	Expression is in the alar neuroepithelium.
TS20: cerebral cortex	Present		2C
TS20: ganglionic eminence	Present		2C
TS20: forebrain	Present	Regionally restricted	2C	Expression is in the hippocampus, olfactory bulb, and the claustrum primordium.
TS20: diencephalon	Strong	Regionally restricted	2C	Expression is in longitudinal and transverse bands, with sharp boundaries.
TS20: midbrain	Present	Regionally restricted	2C	Expression is in the tectum.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Widespread	2D	Expression is in the alar neuroepithelium.
TS20: diencephalon	Strong	Regionally restricted	2D	Expression is in longitudinal and transverse bands, with sharp boundaries.
TS20: midbrain	Present	Regionally restricted	2D	Expression is in the tectum.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present		2E

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	2H
TS21: midbrain	Present	Regionally restricted	2H

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	2I
TS21: midbrain	Present	Regionally restricted	2I

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	2J	Expression is as a gradient in the dorsal part of the neuroepithelium.
TS20: basal columns	Absent		2J

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		2K
TS28: hippocampus CA3	Present		2K
TS28: hippocampus CA4	Present		2K
TS28: thalamic nucleus	Present		2K

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present		3A
TS21: ganglionic eminence	Present		3A
TS21: forebrain	Present	Regionally restricted	3A	Expression is in the hippocampus and the claustrum primordium.
TS21: epithalamus	Present		3A
TS21: olfactory lobe	Present		3A
TS21: diencephalon	Strong	Regionally restricted	3A	Expression is in longitudinal and transverse bands, with sharp boundaries. In the pretectum (p1 prosomere), and the epiphysis in prosomere p2. Expression stopped abruptly at the zona limitans intrathalamica.
TS21: thalamus	Strong	Regionally restricted	3A	Dorsal thalamus, except for a thin ventral band close to the alar/basal boundary.
TS21: midbrain	Present	Regionally restricted	3A	Expression is in the tectum.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present		3B
TS21: ganglionic eminence	Present		3B
TS21: forebrain	Present		3B	Expression is in the hippocampus (strongest) and the claustrum primordium.
TS21: diencephalon	Strong		3B	Expression is in longitudinal and transverse bands, with sharp boundaries.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present		3C
TS21: ganglionic eminence	Present		3C
TS21: forebrain	Present	Regionally restricted	3C	Expression is in the hippocampus (stongest) and the claustrum primordium.
TS21: diencephalon	Strong	Regionally restricted	3C	Expression is in longitudinal and transverse bands, with sharp boundaries.
TS21: epithalamus	Strong		3C
TS21: thalamus	Strong	Regionally restricted	3C	Dorsal thalamus, except for a thin ventral band close to the alar/basal boundary.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Strong	Regionally restricted	3D	Expression is in longitudinal and transverse bands, with sharp boundaries.
TS21: thalamus	Strong	Regionally restricted	3D	Dorsal thalamus, except for a thin ventral band close to the alar/basal boundary.
TS21: epithalamus	Strong		3D

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Strong	Regionally restricted	3E	Expression is in longitudinal and transverse bands, with sharp boundaries.
TS21: thalamus	Strong	Regionally restricted	3E	Dorsal thalamus, except for a thin ventral band close to the alar/basal boundary.
TS21: epithalamus	Strong		3E

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Strong	Regionally restricted	3F	Expression is in longitudinal and transverse bands, with sharp boundaries.
TS21: thalamus	Strong	Regionally restricted	3F	Dorsal thalamus, except for a thin ventral band close to the alar/basal boundary.
TS21: epithalamus	Strong		3F

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Absent		4F

Structure	Level	Pattern	Image	Note
TS22: optic stalk	Absent		4G

Structure	Level	Pattern	Image	Note
TS21: thalamus	Present	Regionally restricted	4H	Expression is in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	4I	Expression is in nuclei in the ventral midbrain and hindbrain
TS21: midbrain	Present	Regionally restricted	4I	Expression is in nuclei in the ventral midbrain and hindbrain
TS21: metencephalon	Present		4I
TS21: midbrain-hindbrain junction	Present	Regionally restricted	4I	Expression was thinned at the junction.

Structure	Level	Pattern	Image	Note
TS27: inferior colliculus	Very strong	Regionally restricted	4L
TS27: superior colliculus	Strong	Regionally restricted	4L

Structure	Level	Pattern	Image	Note
TS28: deep cerebellar nucleus	Present

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	5_E11.5 Lhx9	Expression is in the differentiating layers of the neuroepithelium, as opposed to the mitotic ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	5_E13.5 Lhx9
TS21: cerebral cortex ventricular layer	Absent		5_E13.5 Lhx9

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Absent		5_E16.5 Lhx9

Structure	Level	Pattern	Image	Note
TS27: neocortex layer	Absent		5_P1 Lhx9

Structure	Level	Pattern	Image	Note
TS27: hippocampus CA1	Absent
TS27: hippocampus CA2	Absent

Structure	Level	Pattern	Image	Note
TS19: urogenital ridge	Present	Regionally restricted	6A	Expression in caelomic cavity at level of urogenital ridge, including the gonads.
TS19: pancreas primordium epithelium	Present	Regionally restricted	6A	Expression in parts of the pancreas.
TS19: liver	Present	Regionally restricted	6A	Expression in liver epithelium.

Structure	Level	Pattern	Image	Note
TS19: future pituitary	Absent
TS19: nasal epithelium	Absent

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Graded	6B	Distal mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Graded	6C	Distal mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: hindlimb interdigital region	Present		6D
TS22: hindlimb digit mesenchyme	Present	Regionally restricted	6D	Expression is surrounding the cartilage.

Structure	Level	Pattern	Image	Note
TS24: forelimb interdigital region	Present		6E
TS24: forelimb digit mesenchyme	Present	Regionally restricted	6E	Expression is surrounding the cartilage.