Probe:	IMAGE clone 575916
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present	Regionally restricted	2G	Expression is in the stromal cells, in the periphery of the metanephros and surrounding the ureteric and nephrogenic tubules.
TS21: gland	Present	Regionally restricted	2G	Expression is in the adrenal cortex.

Structure	Level	Pattern	Image	Note
TS23: immature loop of Henle	Strong		2H

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present	Regionally restricted	4A	Expression is ventral.
TS12: midbrain-hindbrain junction	Present		4A

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present		4B
TS13: future prosencephalon	Present		4B
TS13: midbrain-hindbrain junction	Present		4B
TS13: rhombomere 4	Strong		4B

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		4C
TS15: future hindbrain	Present		4C
TS15: olfactory placode	Present		4C
TS15: cranial preganglion	Present		4C	Expression is in the epibranchial placodes.
TS15: head	Present	Regionally restricted	4C	Expression is surrounding the dorsal optic vesicle.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		4D
TS15: future hindbrain	Present		4D
TS15: cranial preganglion	Present		4D	Expression is in the epibranchial placodes.
TS15: head	Present	Regionally restricted	4D	Expression is surrounding the dorsal optic vesicle.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		4E
TS17: medial-nasal process	Present		4E
TS17: telencephalon	Present		4E
TS17: olfactory placode	Present		4E
TS17: trunk	Present	Regionally restricted	4E	Expression is in the ventral body wall overlying the peritoneal cavity.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		4F
TS17: telencephalon	Present		4F
TS17: olfactory placode	Present		4F
TS17: trunk	Present	Regionally restricted	4F	Expression is in the ventral body wall overlying the peritoneal cavity.
TS17: head	Present	Regionally restricted	4F	Expression is surrounding the dorsal optic vesicle.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	4G	Expression is in a striated pattern in the ventral hindbrain.
TS19: telencephalon	Present		4G

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	4H	Expression is in a striated pattern in the ventral hindbrain.
TS19: telencephalon	Present		4H

Structure	Level	Pattern	Image	Note
TS17: eye	Absent		5A

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present	Regionally restricted	5B	Lens fiber cells.
TS20: pigmented retinal epithelium	Present		5B

Structure	Level	Pattern	Image	Note
TS22: lens fiber	Present		5C
TS22: pigmented retinal epithelium	Present		5C

Structure	Level	Pattern	Image	Note
TS23: lens fiber	Present		5D
TS23: pigmented retinal epithelium	Present		5D
TS23: lens anterior epithelium	Strong		5D

Structure	Level	Pattern	Image	Note
TS23: brain	Strong		7A
TS23: central nervous system ganglion	Strong		7A
TS23: cochlear ganglion	Strong		7A
TS23: cranial ganglion	Strong		7A
TS23: molar mesenchyme	Strong		7A
TS23: olfactory epithelium	Strong		7A
TS23: salivary gland	Strong		7A
TS23: telencephalon	Strong		7A

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Strong		7D

Structure	Level	Pattern	Image	Note
TS23: cochlea epithelium	Present		7G
TS23: cochlear ganglion	Present		7G

Structure	Level	Pattern	Image	Note
TS21: midgut	Present		8A
TS21: midgut derived duodenum	Present		8A