About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:29932 Fedtsova NG, et al., Brn-3.0 expression identifies early post-mitotic CNS neurons and sensory neural precursors. Mech Dev. 1995 Nov;53(3):291-304
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5468308
Gene symbol: Pou4f1
Gene name: POU domain, class 4, transcription factor 1
Antibody: anti-Brn3.0
Detection system: fluorescein
Assay notes: Specimens were fixed in 60% ethanol and 4% formaldehyde.
Results
Specimen 2c: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 2c Expression in two groups of mesencephalic neurons, including the mesencephalic nucleus of the trigeminal (MesvV) and in migrating cells in the vestibulocochlear nerve.
Specimen 2d: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: dorsal root ganglion Present Regionally restricted 2d Expression in migrating cells in the dorsal root ganglia.
Specimen 2e: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: trigeminal preganglion Present 2e
TS15: facio-acoustic preganglion complex Present Regionally restricted 2e Expression in migrating cells in the vestibulocochlear nerve complex.
Specimen 2f: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 2f Expression in a group of post-mitotic neurons in ventral part of caudal pons/rostral medulla.
Specimen 4a: embryonic day 13.5 (more )
Note: Double labeled: green - Pou4f1; red - Pcna (assay MGI:5468309).
Structure Level Pattern Image Note
TS21: midbrain Present Regionally restricted 4a Expression in large number of neurons in the midbrain tectum.
Specimen 4c: embryonic day 13.5 (more )
Note: Double labeled: green - Pou4f1; red - Pcna (assay MGI:5468309).
Structure Level Pattern Image Note
TS21: midbrain Present Regionally restricted 4c Expression in the midbrain tectum.
Specimen 4d: embryonic day 9.5 (more )
Note: Double labeled: green - Pou4f1; red - Pcna (assay MGI:5468309).
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 4d Expression in the midbrain tectum.
Specimen 4e: embryonic day 9.5 (more )
Note: Double labeled: green - Pou4f1; red - BrdU.
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 4e Expression in the midbrain tectum.
Specimen 4f: embryonic day 9.5 (more )
Note: Double labeled: green - Pou4f1; red - Pcna (assay MGI:5468309).
Structure Level Pattern Image Note
TS15: midbrain-hindbrain junction Present Regionally restricted 4f Expression in cells of the mesencephalic nucleus of the trigeminal (MesV) located at the midbrain hindbrain junction.
Specimen 4g: embryonic day 10.5 (more )
Note: Double labeled: green - Pou4f1; red - Pcna (assay MGI:5468309).
Structure Level Pattern Image Note
TS17: hindbrain Present Regionally restricted 4g Expression in a group of post-mitotic neurons in ventral part of caudal pons/rostral medulla.
Specimen 4h: embryonic day 15.5 (more )
Note: Double labeled: green - Pou4f1; red - Pcna (assay MGI:5468309).
Structure Level Pattern Image Note
TS23: neural retina Present Regionally restricted 4h Expression in ganglion cells forming a thick layer of differentiating neurons within the proliferating neuroepithelium.
Specimen 5a: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: trigeminal ganglion Present 5a
Specimen 5b: embryonic day 10.5 (more )
Note: Double labeled: green - Pou4f1; red - BrdU.
Structure Level Pattern Image Note
TS17: trigeminal ganglion Present 5b
Specimen 5d: embryonic day 10.5 (more )
Note: Double labeled: green - Pou4f1; red - BrdU.
Structure Level Pattern Image Note
TS17: trigeminal ganglion Present 5d
Specimen 5e: embryonic day 9.5 (more )
Note: Double labeled: green - Pou4f1; red - BrdU.
Structure Level Pattern Image Note
TS15: dorsal root ganglion Present 5e
Specimen 6a/b: embryonic day 10.0 (more )
Note: 27 somites. Double labeled: green - Pou4f1; red - Nefm (assay MGI:5468310).
Structure Level Pattern Image Note
TS15: midbrain-hindbrain junction Present 6a , 6b
Specimen 6c: embryonic day 10.0 (more )
Note: 27 somites. Double labeled: green - Pou4f1; red - Nefm (assay MGI:5468310).
Structure Level Pattern Image Note
TS15: dorsal root ganglion Present 6c
Specimen 6d: embryonic day 10.5 (more )
Note: 40 somites. Double labeled: green - Pou4f1; red - Nefm (assay MGI:5468310).
Structure Level Pattern Image Note
TS18: dorsal root ganglion Present 6d
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory