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Gene Expression Data
Assay Details
Assay
Reference: J:29932 Fedtsova NG, et al., Brn-3.0 expression identifies early post-mitotic CNS neurons and sensory neural precursors. Mech Dev. 1995 Nov;53(3):291-304
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5468309
Gene symbol: Pcna
Gene name: proliferating cell nuclear antigen
Antibody: PC10
Detection system: fluorescein
Assay notes: Specimens were fixed in 60% ethanol and 4% formaldehyde.
Results
Specimen 4a/b/c: embryonic day 13.5 (more )
Note: Double labeled: red - Pcna; green - Pou4f1 (assay MGI:5468308).
Structure Level Pattern Image Note
TS21: midbrain Present Regionally restricted 4a , 4c , 4b Expression in virtually all cells in the neuroepithelium. Faint residual expression sometimes visible in post-mitotic cells adjacent to neuroepithelial layer.
Specimen 4d: embryonic day 9.5 (more )
Note: Double labeled: red - Pcna; green - Pou4f1 (assay MGI:5468308).
Structure Level Pattern Image Note
TS15: future midbrain Present Widespread 4d Expression in virtually all cells in the neuroepithelium.
Specimen 4f: embryonic day 9.5 (more )
Note: Double labeled: red - Pcna; green - Pou4f1 (assay MGI:5468308).
Structure Level Pattern Image Note
TS15: midbrain-hindbrain junction Present 4f
Specimen 4g: embryonic day 10.5 (more )
Note: Double labeled: red - Pcna; green - Pou4f1 (assay MGI:5468308).
Structure Level Pattern Image Note
TS17: hindbrain Present 4g
Specimen 4h: embryonic day 15.5 (more )
Note: Double labeled: red - Pcna; green - Pou4f1 (assay MGI:5468308).
Structure Level Pattern Image Note
TS23: retina Present 4h
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory