Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43464
Matise MP, et al., Expression patterns of developmental control genes in normal and Engrailed-1 mutant mouse spinal cord reveal early diversity in developing interneurons. J Neurosci. 1997 Oct 15;17(20):7805-16
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5467551
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Gene symbol:
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Lhx5
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Gene name:
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LIM homeobox protein 5
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Antibody:
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4F2
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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The antibody used in this assay recognizes two genes.
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Results
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Specimen
5b & 5c:
embryonic day 9.5
(more  )
Note: Double labeled: green - Lhx1 and Lhx5; red - En1 (assay MGI:5467545)
Specimen
5b & 5c:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Lhx1 and Lhx5; red - En1 (assay MGI:5467545)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future spinal cord |
Present |
Regionally restricted |
5b
,
5c
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Expression was detected in the intermediate region. At the ventral limit of expression, a subset of expressing cells also express En1.
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Specimen
5d:
embryonic day 11.5
(more )
Note: Double labeled: green - Lhx1 and Lhx5; red - En1 (assay MGI:5467545)
Specimen
5d:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Lhx1 and Lhx5; red - En1 (assay MGI:5467545)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
5d
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Expression was detected in interneurons. Some co-expression was noted with En1, but the ventral-most cells did not co-express En1 at this stage.
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| TS19: sclerotome |
Absent |
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5d
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Specimen
7a & 7c:
embryonic day 9.5
(more )
Note: Double labeled: green - Lhx1 and Lhx5; red - Pax2 (assay MGI:5467552)
Specimen
7a & 7c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Lhx1 and Lhx5; red - Pax2 (assay MGI:5467552)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future spinal cord |
Present |
Regionally restricted |
7a
,
7c
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In midthoracic spinal cord, expression in the ventral intermediate region overlaps with Pax2.
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Specimen
7d & 7f:
embryonic day 9.5
(more )
Note: Double labeled: green - Lhx1 and Lhx5; red - Pax2 (assay MGI:5467552)
Specimen
7d & 7f:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Lhx1 and Lhx5; red - Pax2 (assay MGI:5467552)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future spinal cord |
Present |
Regionally restricted |
7f
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7d
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Expression was detected in dorsal and ventral domains and overlaps with Pax2. Staining in the ventral intermediate region is ventral to the sulcus limitans.
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Specimen
not shown:
embryonic day 11.5
(more )
Note: Also stained for Pax2 (assay MGI:5467552)
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Also stained for Pax2 (assay MGI:5467552)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
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Expression in the ventral horn showed less overlap with Pax2 at E11.5. However, many cells in the intermediate region continue co-expression. There is only partial overlap of expression with Pax2 in the dorsal horn.
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